Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for detecting antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 5-h treatment of samples by placing them in water dosed with sulfadimethoxine (SDM; 200 g/ton water), the residue depletion of SDM was investigated in 25 cultured olive flounders (Paralichthys olivaceus). Muscles from fish were sampled before treatment and on the 1st, 2nd, 3rd, 4th and 5th days after treatment. The concentration of SDM in the muscle was then determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the residue in olive flounder muscle. To investigate the recovery rate, standard solutions were added to muscle samples to obtain final concentrations of 25 and 50 ng/mL in the muscle. The recovery rates of all spiked samples were >96.6% of the spiked value. SDM was detectable in the muscle of fish treated with the drug until the 1st day of the withdrawal period. The present study shows that the LFIA can be easily adopted to detect SDM residues in the tissue of farmed fish.

Antibiotic Detection Kit (Combination I), a lateral flow immunoassay (LFIA) developed for the detection of antibiotic residues in milk, was utilized for the analysis of antibiotic residues in the muscle tissue of olive flounder. After 60-min treatment by dipping in water dosed with ampicillin (200-g/ton water), the residue depletion of ampicillin was investigated in 25 cultured olive flounder (Paralichthys olivaceus). Muscles of fish were sampled on the 1st, 2nd, 3rd, 4th and 5th day after drug treatment. The concentration of ampicillin in the muscle was determined by LFIA. The absorbance ratio of the sample to the control blank (Bs/Bo) was employed as an index to determine the muscle residues in olive flounder. To investigate the recovery rate, standard solutions were added to muscle samples to give final concentrations in the muscle of 4 and 8 ng/ml. The recovery rates of all spiked samples were > 96% of the spiked value. Ampicillin was detected in the muscle of fish treated with the drug until the 2nd day of the withdrawal period. The present study showed that the LFIA can be easily adopted to predict ampicillin residues in tissue of farmed fishes.

“Shinmichal1”, a waxy red winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “Alchan”/“Chal2”during 1997. “Alchan” is a soft red winter wheat with high yield and good noodle quality. “Chal2” is a waxy wheat line crossed by “Kanto107” (Wx-A1b and Wx-B1b) and “BaiHuo” (Wx-D1b). “Shinmichal1” was evaluated as “Suwon306” in Advanced Yield Trial Test in 2002. It was tested in the regional yield trial test between 2003 and 2006. “Shinmichal1” is an awned, semi dwarf and soft red winter wheat, similar to “Uri” (check cultivar). The Heading and maturing date of “Shinmichal1” were similar to “Uri”. Culm and spike length of “Shinmichal1“ were 79 cm and 7.8 cm. “Shinmichal1“ had greater test weight (802 g) and lower 1,000-grain weight (31.8 g) than “Uri” (782 g and 35.2 g, respectively). It has resistance to winter hardiness, wet-soil tolerance, lodging tolerance and moderate post-harvest sprouting damage. “Shinmichal1” had higher flour yield (66.2%), ash (0.73%), protein (10.9%) and SDS sedimentation volume (40.7 ml) than “Uri” (64.7%, 0.53%, 9.3%, and 32.5 ml, respectively) and similar flour color to “Uri”. “Shinmichal1” had higher dough extensibility and lower dough strength than “Uri”. It is 5.2% of amylose content and had typical waxy starch pasting properties. Average yield of “Shnmichal1” in the regional adaptation yield trial was 5.24 MT ha-1 in upland and 5.58 MT ha-1 in paddy field, which was 3% and 8% lower than those of “Uri” (5.39 MT ha-1 and 6.09 MT ha-1, respectively). “Shinmichal1” would be suitable for the area above the daily minimum temperature of -10℃ in January in Korean peninsula.