충북의 한지형 마늘 재배지에서 파총채벌레와 파좀나방의 발생양상과 약제검정을 수행하였다. 파총채벌레 발생양상은 3월 중순에 발생하기 시작하여 5월 중하순경 부터 밀도가 급격히 증가하였다. 파좀나방은 3월 중하순에 발생이 시작되어 수확기인 6월 중순까지 2화기 발생하였다. 9종의 살충제를 이용하여 파총채벌레와 파좀나방의 약제감수성을 조사하였다. 실내 검정 결과 파총채벌레에 대하여 clothianidin SC, chlorfenapyr EC, dimethoate EC, imidacloprid WP, cyantraniliprole + thiamethoxam WG가 100%의 살충효과를 보였다. 파좀나방에 대해서는 cyantraniliprole EC, pyridalyl EW, spinetoram SG, abamectin + chlorantraniliprole SC가 100%의 살충효과를 보였다. 2종 해충에 대하여 실내검정에서 살충효과가 높았던 약제 각각 5, 4종을 대상으로 포장검정을 수행한 결과 모든 약제에서 처리 3일차에서 90% 이상의 방제효과를 나타내었다.

For the setting of control timing, the number of overwintering eggs on host plants and population density of Metcalfa pruinosa nymphs and adults was surveyed in a sesame fields. According to a survey of the overwintering eggs density of M. pruinosa on host plants, they were many in the order of Mugunghwa trees, Oak trees, and Acacia trees. Examined sesame branches within the 50cm was not found population densities of M. pruinosa nymphs and adults, and it was also very low in the sticky trap. The peak density of the adult was highest at 0.13 per trap on 11 after being first investigated on July 3 in a string wrap survey, and then decreased. In a sesame fields, it is judged that the control of the M. pruinosa is due to be 35 days after the end of the insect hatching, and that distribution of host plants is more important in the outside region than inside region.

Whitefly (Hemiptera: Aleyrodidae) pests, including the sweetpotato whitefly, Bemisia tabaci, and the greenhouse whitefly, Trialeurodes vaporariorum, are economically important in agriculture. With the annual growth of the domestic fresh fruit export market, various quarantine treatment methods are being used to export strawberries of better quality. The purpose of the present study was to investigate the effect of ionizing radiations (electron beam, X-ray, gamma-ray) on the development and reproduction of two species of whitefly and to determine the optimal dose of three ionizing radiations for the construction of quarantine technology applicable to strawberry export.

The cotton bollworm, Helicoverpa armigera, is an economically important pest, which harms various kinds of important agricultural crops, such as tomato. The purpose of the present study was to investigate the effect of ionizing radiations (electron beam, X-ray, gamma-ray) on the development and reproduction of H. armigera and to determine the optimal dose of three ionizing radiations for the construction of quarantine technology applicable to tomato export.

The fumigation toxicity of carbonyl sulfide to T. castaneum as a storage grain pest was evaluated. Carbonyl sulfide (COS) is registered in Australia for microorganism present in soil, root and fertilizer. the fumigation activity of carbonyl sulfide was investigated in 12 L desiccator for 24 h exposure to eggs, larvae, pupae, adults of T. castaneum. Eggs and pupae were showed 87.3% and 95.6% mortality for 25 mg/L of COS, respectively. Larvae and adults were investigated with 80.0% and 100.0% mortality at 15 mg/L treatment, respectively. Therefore, the eggs of T. castaneum showed the highest tolerance to COS.

The fumigation activity of phosphine (PH3) to T. castaneum as a storage grain pest was evaluated. The lethal concentration time (LCT) value of each developmental stage (egg, early larva, late larva, pupa and adult) of T. castaneum was analyzed in 12 L desiccator. At the T. castaneum larva stages, exposure for 4 h showed low LCT value, especially in early stage larvae (LCT99 = 0.32 mg·h/L) which is very high susceptibility to PH3. However, T. castaneum eggs were observed very high tolerance to PH3 at LCT99 77.47 mg·h/L. Therefore, the fumigant activity of PH3 against T. castaneum can be found to be significantly different depending on developmental stage.

The two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae), is one of the most important pest species, because it devastates many horticultural and ornamental crops and fruit trees. The resistance ratios calculated for the LC50 value in acequinocyl- and pyridaben-resistant strain was 4,237- and 5,555-fold higher than that of the susceptible strain, respectively. The objective of this study was to evaluate the cross-resistance to several acaricides and to identify the mechanisms associated with acequinocyl- and pyridaben-resistant strain of T. urticae.

Mosquitoes are the most important medical species by transmitting some of deadly infectious diseases to human. Established vector control methods involving the elimination of larval habitats, the use of physical barriers, or those involving insecticides are not sufficient to maintain the populations of these species below the epidemic risk threshold. The sterile insect technique (SIT) is a species-specific, non-polluting and environmentally benevolent method of insect control. This technique involves the release of massive numbers of artificially-reared sterile male insects that, due to their abundance, outcompete fertile wild males for mating opportunities with wild female insects. In this study we discuss optimization of the sterilization process and present a simple model and procedure for determining the optimum dose.

본 연구에서는 세 가지 온도(20, 25, 30℃)에서 꼬마남생이무당벌레의 생물적 특성을 조사하였다. 복숭아혹진딧물 약충을 먹이로 사육한 꼬마남생이무당벌레 암컷의 성충기간은 20, 25, 30℃에서 각각 134.0, 101.0, 55.2일이었고, 총 산란수는 각각 508.6, 875.6, 383.4개였고, 수 컷의 성충기간은 각각 128.8, 97.8 및 46.5일이었다. 25℃에서 꼬마남생이무당벌레의 1령, 2령, 3령, 4령, 수컷 및 암컷의 복숭아혹진딧물 성충 의 일일 평균포식량은 각각 2.2, 7.3, 14.5, 29.1, 35.0 및 42.9마리이었고, 목화진딧물 성충의 일일 평균포식량은 각각 2.2, 7.5, 13.9, 29.5, 37.0 및 40.8마리이었다.

많은 산림해충들은 목재검역에서 문제가 되고 있어 포스핀(PH3)을 이용하여 광릉긴나무좀, 왕녹나무좀, 노랑애나무좀의 성충에 대한 살충 활성을 조사하였다. 3종의 나무좀류에 대하여 99% 살충활성을 보이는 LCT값은 각각 3.192 (광릉긴나무좀), 0.994 (왕녹나무좀), 0.501 mg ‧ h/L (노랑애나무좀) 순으로 나타났다. 포스핀을 처리한 3종의 나무좀류는 시간이 지남에 따라 살충활성도 증가하였다. 특히 광릉긴나무좀에서 는 0.4 mg/L 이상의 농도에서 약제처리 7일 후에 100%의 사충률을 보였다. 이러한 결과로 볼 때 포스핀이 3종의 목재해충 성충에 대해 메칠브 로마이드의 대체훈증제로 이용될 수 있을 것으로 판단된다.

3년간(2016-2018년) 청주지역에서 채집된 모기의 발생분포를 채집지 3곳(주거지와 철새도래지, 축사)에서 2종의 트랩(BL트랩과 BG트 랩)을 이용하여 비교 조사하였다. 주거지에서 가장 많이 채집된 모기 종은 빨간집모기였으며, 철새도래지와 축사에서는 금빛숲모기가 가장 많이 채집되었다. 철새도래지와 주거지에서는 BG트랩이 BL트랩보다 모기가 더 많이 채집되었으나 축사에서는 BL트랩에서 더 많은 모기가 채집되 었다. 빨간집모기와 흰줄숲모기, 큰검정들모기는 BG트랩에서 많이 채집되었고 금빛숲모기, 얼룩날개모기류, 동양집모기는 비교적 BL트랩을 선호하는 것으로 조사되었다. 채집된 모기를 분류 ․ 동정한 결과 2016년에는 10종 22,679개체가 채집되었고, 2017년에는 8종 6,502개체가 채집 되어 2016년보다 3.49배 감소하였다. 2018년에는 6,803개체가 채집되어 2016년보다 3.33배 감소하였다. 이러한 결과가 강수량과 관련이 있을 것으로 보이며 기상조건과 여러 변인들(채집지역과 모기종류, 트랩종류)에 의한 모기밀도와의 관련성을 비교한 결과 특정 관련성을 확인할 수는 없었다. 채집된 모기의 병원체 감염을 확인한 결과, 2016년도 축사에서 채집한 금빛숲모기 2개 pool, 2018년에 축사에서 채집한 빨간집모기 1개 의 pool에서 차오양바이러스(chaoyang virus)가 검출되어 각각 0.088와 0.147의 최소감염율(MIR)을 보였다. 2017년에는 바이러스가 검출되 지 않았다.

We assessed the susceptibility of three fumigants on Phthorimaea operculella, which is an important pest of stored potato worldwide. 5 to 6 initial dosage of each fumigants were treated on every growth stages of P. operculella. Methyl bromide showed 100% mortality at CT 33.40mg h/ L on egg, CT 14.41mg h/L on late larvae, CT 31.89mg h/L on pupae and CT 16.01mg h/L on adult, respectively. The LCT50 of methyl bromide was 19.115mg h/L on egg, 3.934mg h/L on late larvae, 13.810mg h/L on pupae and 6.260mg h/L on adult, respectively. In case of phosphine, 98% mortality was achieved at CT 16.77mg h/L on egg, and 100% mortality was achieved at CT 16.58mg h/L on late larvae, CT 18.54mg h/L on pupae and CT 12.28mg h/L on adult, respectively. The LCT50 of phosphine was 1.457mg h/L on egg, 2.236mg h/L on late larvae, 1.282 mg h/L on pupae and 0.253mg h/L on adult, respectively. In case of ethyl formate, 100% mortality was achieved at CT 96.21mg h/L on egg, CT 101.30mg h/L on late larvae, CT 120.66mg h/L on pupae and CT 148.30mg h/L on adult, respectively. The LCT50 of ethyl formate was 23.730mg h/L on egg, 13.706 mg h/L on late larvae, 29.578mg h/L on pupae and 19.235mg h/L on adult, respectively.

Carbonyl sulfide(COS) is a naturally generated gas from fermentation process of microbes and from plant root and stem. COS was firstly registered as a fumigant at 1993 to control stored product pests. To supplement environmental problems and toxicity of commercial fumigants and develop new fumigant, we have processed the susceptibility assessment of carbonyl sulfide on important agricultural pests, Myzus persicae and Tetranychus urticae. Every growth stages of two insect species were tested, and five dosages of carbonyl sulfide were treated for 4 hours, and the mortality was investigated after 24 hours of treatment. Nymphal stage of M. persicae was completely controlled at more than 20 mg/L dosage, and adult stage showed 95.8% mortality at 80 mg/L dosage. The LC50 of M. persicae was 7.314mg/L for nymph and 26.117mg/L for adult stage. Egg stage of T. urticae showed 91.2% mortality when treated with 100mg/L carbonyl sulfide, and nymph and adult stage showed 100% and 94.1% mortality at 8mg/L and 80mg/L, respectively. The LC50 of T. urticae was 73.110mg/L for egg, 2.818mg/L for nymph and 12.054mg/L for adult stage.

This study investigated the fumigant activity of phosphine (PH3) on 2 kinds of mealybug (Pseudococcus longispinus and P. orchidicola (Hemiptera: Pseudococcidae)) adults and nymphs. All of the two mealybugs adults showed higher LCT99 values than nymphs, and P. longispinus showed higher tolerance than P. orchidicola in a 12 L desiccator. The absorption of phosphine on 13 nursery plants showed 12.2~41.5% difference depending on the plant. All of the mealybugs treated with phosphine 2 mg/L in 0.5 m3 fumigation chamber for 4 h showed 100% fumigant activity, except P. longispinus adult (approximately 90% at bottom part). However, when the exposure time was increased to 24 h, all of them showed 100% mortality. In the treatment of 10 m3 container, the 24 h treatment of phosphine showed 100% mortality to P. longispinus and P. orchidicola adults and nymphs. In all the experiments, no phytotoxicity of phosphine observed on 13 plants until 1 month after treatment.