As the importance of radioactive waste management has emerged, quality assurance management of radioactive waste has been legally mandated and the Korea Radioactive Waste Agency (KORAD) established the “Waste Acceptance Criteria for the 1st Phase Disposal Facility of the Wolsong Lowand Intermediate-Level Waste Disposal Center (WAC)”, the detailed guideline for radioactive waste acceptance. Accordingly, the Korea Atomic Energy Research Institute (KAERI) introduced a radioactive waste quality assurance management system and developed detailed procedures for performing the waste packaging and characterization methods suggested in the WAC. In this study, we reviewed the radioactive waste characterization method established by the KAERI to meet the WAC presented by the KORAD. In the WAC, the characterization items for the disposal of radioactive waste were divided into six major categories (general requirements, solidification and immobilization requirements, radiological, physical, chemical, and biological requirements), and each subcategories are shown in detail under the major classification. In order to satisfy the characterization criteria for each detailed item, KAERI divided the procedure into a characterization item performed during the packaging process of radioactive waste, a separate test item, and a characterization item performed after the packaging was completed. Based on the KAERI’s radioactive waste packaging procedure, the procedure for characterization of the above items is summarized as follows. First, during the radioactive waste packaging process, the characterization corresponding to the general requirements (waste type) is performed, such as checking the classification status of the contents and checking whether there are substances unsuitable for disposal, etc. Also, characterization corresponding to the physical requirements is performed by checking the void fraction in waste package and visual confirmation of particulate matter, substances containg free water, ect. In addition, chemical and biological requirements can be characterized by visually confirming that no hazardous chemicals (explosive, flammable, gaseous substances, perishables, infectious substances, etc.) are included during the packaging process, and by taking pictures at each packaging steps. Items for characterization using separate test samples include radiological, physical, and chemical requirements. The detailed items include identification of radionuclide and radioactivity concentration, particulate matter identification test, free water and chelate content measurement tests, etc. Characterization items performing after the packaging is completed include general requirements such as measuring the weight and height of packages and radiological requirements such as measurements of surface dose rate and contamination, etc. All of the above procedures are proceduralized and managed in the radioactive waste quality assurance procedure, and a report including the characterization results is prepared and submitted when requesting acceptance of radioactive waste. The characterization of KAERI’s radioactive waste has been systematically established and progressed under the quality assurance system. In the future, we plan to supplement various items that require further improvement, and through this, we can expect to improve the reliability of radioactive waste management and activate the final disposal of KAERI’s radioactive waste.

We investigated if Pseudococcus viburni and P. longispinus, the two notorious pest species of mealybugs are foundin Korea. Our survey involved a total of 602 sites all over the country for the last three years. Pseudococcus viburniwas only once referred in Gimpel’s 1983 dissertation on P. affinis group: only one record was noted from Korea amongmany specimens from various countries. However, the species has never been reported from Korea for the last severaldecades and none was found in our thorough survey. We believe there was a misidentification, as noted by the authorregarding the species complex, and conclude that P. viburni does not occur in Korea. On the other hand, P. longispinushas been found in 36 sites, all from imported tropical houseplants in a green house or nurseries. In addition, anothersimilar species has been found from 86 sites. This unknown species was surprisingly best matched with P. orchidicola.Both species could not survive Korea’s harsh winter and they could only survive in a temperature-controlled place suchas nurseries and restaurants.

This study was conducted to establish the optimal chemical post-activation conditions in porcine embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) using 4 different chemical compositions (cytochalasin B (CB), cyclohexamide (CHX), demecolcine (DC), 6-dimethylaminopurine (DMAP). Porcine embryos were produced by PA and SCNT and then, cultured for post-activation with CB (7.5 μg/mL), CB (7.5 μg/mL) + CHX (10 μg/mL), CB (7.5 μg/mL) +DC (0.4 μg/mL), and CB (7.5 μg/mL) + DMAP (2 mM). In PA embryonic development, cleavage rates have been significantly higher in CB group (94.7%) and CB+DMAP group (94.1%) than that of CB+CHX and CB+DC group (88.1 and 84.3%, respectively). There have been no significant differences in blastocyst formation rates among the four groups. In cell number of blastocyst was shown in CB group (42.3%) significantly higher than CB+CHX and CB+DC group (40.6 and 40.6%, respectively). In SCNT embryonic development, CB+DMAP group (89.7%) significant differences were found on embryo cleavage rates when compared with other three groups. Blastocyst formation rates in CB+DMAP group (26.9%) were significantly higher when compared with CB, CB+CHX, and CB+DC groups (25.5, 20.2, and 22.1%, respectively). In blastocyst cell number, CB+DMAP group (41.4%) was found higher significant difference compared with other three groups. Additionally, we have investigated survivin expression in early development stages of porcine SCNT embryos for more confirmation. Our results establish that CB group and CB+DMAP group for 4 h during post-activation improves pre-implantation improvement of PA and SCNT embryos.

Pseudococcus longispinus, a notorious cosmopolitan pest species of mealybugs, known to be distributed indoors in Korea since 2002, is found to be mixed with another species, Pseudococcus orchidicola. Finding P. orchidicola as a pest of tropical plants in Korea is rather unexpected because of their main distribution in Pacific area and South Asia. However, all the available information from morphology, molecule and advices on identification from mealybug specialists indicated this is best matching P. orchidicola. Morphological, molecular and some biological notes on P. orchidicola are provided with some adult and nymphal images, and compared with P. longispinus. A full discussion mainly on identity and distribution of P. orchidicola is given.