Fucoidan has been extensively studied as medicinal materials due to its biological activities including osteoblastic differentiation effect. However, osteoblastic effect by fucoidan is unknown in alveolar bone marrow derived mesenchymal stem cells (ABM-MSCs). The present study was undertaken to evaluate the effect of fucoidan on Osteoblastic differentiation in ABM-MSCs and explore its mechanism. Cell proliferation was analyzed by crystal violet staining. Osteoblast differentiation was determined by alkaline phosphatase activity, calcium accumulation assay and gene expression of osteoblast markers. We found that fucoidan induced cell proliferation of ABM-MSCs. Furthermore, fucoidan increased the ALP activity, calcium accumulation, and osteoblast specific genes such as Runx2, type I collagen alpha 1. Moreover, fucoidan induces the expression of asporin and bone morphogenic protein (BMP)-2 and asporin. Based on these results, these finding indicate that fucoidan induces osteoblast differentiation in ABM-MSCs and partially enhanced the mRNA expression of BMP-2 and asporin.

우리 나라 논에서 분리된 Azospirillum 균주로부터 메가플라스미드를 확인한 결과 Azospirillum lipoferum AS192로부터 5개의 메가플라스미드를 확인했다. Azospirillum lipoferum AS192와 Azospirillum brasilense AS112의 메가플라스미드가 Rhizobium meliloti의 nod ABC와 유사성을 갖고 있다는 사실을 알았으며, 이 결과로부터 토양 미생물과 식물체 간의 관계의 초기 과정에 공통적인 기작이 존재한다고 추정할 수 있었다.