목적: 연구에서는 HMD VR 사용 시 눈에 발생하는 건조증 변화를 파악하기 위해 눈 내부의 온도와 습도의 변화를 관찰하고자 하였다. 방법 : 연구 대상자는 신체 건강한 체육계열 대학생으로 선정하였고 안구건조증의 영향이 있거나 안질환 병력, 수술자와 양안시기능 이상자는 제외하여 최종 연구 대상자는 49명으로 선정되었다. 체육계열 대학생의 HMD 내부 온도, 습도, 체감온도의 변화를 50분간 관찰하였고 VR 사용 전후로 맥모니 테스트(McMonnies test)를 실시하 였다. 결과 : HMD 내부의 온도는 지속적으로 증가하였고, 40분 이후에는 유의미한 차이가 없었다. 습도는 사용 직후 급격히 증가한 후 감소하였으며, 25분 이후에는 초기 베이스라인과 비교하여 유의미한 차이가 없었다. 지속적인 체 감온도 상승은 안구건조증 증상뿐만 아니라 시각적 불편감을 줄 수 있다. 결론 : HMD 방식의 VR 기기의 설계는 안구내 습도환경과 열적 불쾌감을 고려하여 사용자의 편안함과 장기적 인 눈 건강을 보장하기 위한 지속적인 연구와 실험적 검증이 제시되어야 한다.

This study aims to systematically investigate the insect diversity within the distinct coastal grassland and mountain habitats of East Busan, Busan Metropolitan City. The objective is to gather foundational data that will inform natural environment conservation plans and contribute to policy formulation. Employing a comprehensive collection approach, we utilized trapping nets, pitfall traps, and flying insect traps to capture a broad spectrum of insect species. The investigation identified a rich diversity of 132 species across 9 orders and 52 families. Coleoptera emerged as the most prevalent order with 13 families and 41 species, followed by Diptera with 8 families and 24 species, and Lepidoptera with 9 families and 21 species. To assess the biodiversity and structure of these communities by habitat type, we applied several biodiversity indices: the Shannon-Wiener index (H'), Margalef index (R), and Pielou evenness index. Coastal grasslands exhibited an H' of 3.153, R of 11.08, and a Pielou evenness of 0.371; coastal mountains showed an H' of 2.922, R of 13.02, and a Pielou evenness of 0.202. These metrics reveal no significant differences in diversity and evenness between the two habitats. The endangered Copris tripartitus was notably observed in coastal grasslands, alongside 20 species classified as Least Concern (LC) in the National Red List across both habitats. The comparative analysis of biodiversity and evenness levels across coastal grassland and mountain areas highlights the ecological significance of both habitats in supporting a diverse insect fauna. The detection of endangered and LC species within these areas emphasizes the critical need for ongoing monitoring and the development of effective conservation strategies tailored to protect Busan's coastal ecosystems. The findings underscore the necessity of conducting periodic surveys to monitor faunal changes and inform conservation efforts actively. By ensuring the continued protection of insect diversity, these efforts will play a pivotal role in maintaining the ecological integrity of coastal areas, thereby supporting broader environmental conservation objectives.

This study aimed to collect foundational data essential for devising a natural environment conservation plan and implementing relevant policies. It underscores the significance of understanding the current state of the natural environment. Our research concentrated on surveying the biodiversity and distribution of key natural habitats within Busan Metropolitan City, with the goal of gathering crucial data to guide the development of conservation and management strategies. The focus of our investigation was on the insect populations in Ilgwang, covering two primary habitats: mountains and wetlands. The survey, conducted from July to September 2023, utilized various collection methods, including pitfall traps, fly traps, sweeping nets, and aspirators. Our findings documented the presence of 109 species across 88 genera, 50 families, and 9 orders. Specifically, the study identified two species from the order Odonata, two from Blattodea, one from Mantodea, six from Orthoptera, eleven from Hemiptera, twenty-nine from Coleoptera, twelve from Diptera, ten from Lepidoptera, and thirty-five from Hymenoptera. Through community analysis, biodiversity indices were calculated, revealing a Shannon-Wiener index (H') of 4.07 for mountain habitats and 3.92 for wetlands. The Margalef richness index (R) displayed values of 13.92 for mountains and 14.78 for wetlands, while the Pielou evenness index indicated values of 0.96 and 0.91, respectively. Among the identified species, one was classified as endangered, and thirteen were designated as of Least Concern, according to legal protection statuses.

The study of blowfly (Diptera, Calliphoridae) biodiversity and distribution is crucial for forensic investigations involving cadavers. Abiotic and biotic factors, such as seasonality and habitat type, have significant impacts on blowfly populations. However, few forensic entomology studies have been conducted in South Korea, particularly the Gyeongsangnam-do region. To address this, in this study, an extensive year-long survey was conducted to analyze the compositions, habitat preferences, distribution, and seasonal abundance of forensically relevant blowflies in urban and forested habitats in Gyeongsangnam-do, utilizing mouse carcass-baited traps set for 48 h each month. A total of 3,478 adult blowflies were recorded, encompassing six genera and 14 species, with a noted absence of specimens during the winter months. The predominant species was Lucilia porphyrina, accounting for 37.1% of the total sample, followed by Chrysomya pinguis (27.5%), Lucilia sericata (7.6%), and Lucilia illustris (7.1%). The species composition was consistent across all surveyed regions; however, seasonal variation in species diversity was evident, with a peak in spring and decline in summer. Notably, certain species exhibited clear preferences for either urban (Calliphora calliphoroides and L. sericata) or forested habitats (L. porphyrina and Ch. pinguis). This pioneering study elucidates the diverse blowfly communities in Gyeongsangnam-do, highlighting significant seasonal and habitat-dependent variations.

Blockchain is an immutable ledger that records transactions and tracks assets using a common communication protocol. It stores a copy of the blockchain and implements a consensus function to verify transactions. Blockchain is applied to industries beyond finance, such as retail, to maintain security and transparency. Consumers with knowledge of blockchain technology are likely to be affected when evaluating products with blockchain embedded, impacting their product evaluation. The study investigates the impact of blockchain technology on consumers' product evaluation and how knowledge of blockchain and product quality moderate its effects.

The entomopathogenic fungus Metarhizium anisopliae is one of potent biological control agents against a variety ofinsect pests. In this study, we investigated enzyme production of M. anisopliae strains A and B. They produced extracellularenzymes for degrading the epidermis of Monochamus alternatus, a crucial mediator of the pinewood nematode Bursaphelenchusxylophilus. With Q-TOF MS/MS analysis, 29 kDa protein, a major band on a SDS-PAGE gel, was identified as subtilisin-likeserine protease PR1A. M. anisopliae A produced an extracellular enzyme more efficiently than M. anisopliae B: however,enzyme activities targeted for the cuticle were comparable. Our results suggest that the two strains of M. anisopliae havethe biological potential against M. alternatus with insecticidal protease production.

The entomopathogenic fungus Metarhizium anisopliae B is a powerful biological control agent against Monochamusalternatus, a crucial mediator of the pinewood nematode Bursaphelenchus xylophilus. In this study, production of destruxins(dtxs), insecticidal cyclic hexadepsipeptides, was monitored in the submerged culture of M. anisopliae B. Three typesof dtxs, i.e., destruxin A, B, and E, were produced during the culture. Among the three dtxs, the production yield ofdestruxin A was best, followed by destruxin B and E. Destruxin A production was increased when pH was controlledat 6.0, whereas production of destruxin E was not affected by the pH control. The highest yield of dtxs A, B, and Ewere 16.4, 7.3, and 6.1 mg L-1, respectively. Considering that process for dtxs production has not been optimized, M.anisopliae B has more powerful implication as a biocontrol agent.

Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the low-calorie food with a high nutritional value. It is also functional food since it contains a material well-known for its medicinal benefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of the study were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culture media for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was 25℃. Malt extract(2%) and yeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium. Among different five log types, highest mycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).

Ski protein is implicated in proliferation/differentiation in a variety of cells. We had previously reported that Ski protein is present in granulosa cells of atretic follicles, but not in preovulatory follicles, suggesting that Ski has a role in apoptosis of granulosa cells. The alternative fate of granulosa cells other than apoptosis is to differentiate to luteal cells; however, it is unknown whether Ski is expressed and has a role in granulosa cells undergoing luteinization. Thus, the aim of the present study was to locate Ski protein in the rat ovary during luteinizationto predict the possible role of Ski. In order to examine the expression pattern of Ski protein along with the progress of luteinization, follicular growth was induced by administration of equine chorionic gonadtropin to immature female rats, and luteinization was induced by human chorionic gonadtropin treatment to mimic luteinizing hormone (LH) surge. While no Ski-positive granulosa cells were present in preovulatory follicle, Ski protein expression was induced in response to LH surge, and was maintained after the formation of the corpus luteum (CL). Though Ski protein is absent in granulosa cells of preovulatory follicle, its mRNA (c-Ski)was expressed and the level was unchanged even after LH surge. Taken together, these results demonstrated that Ski protein expression is induced in granulosa cells upon luteinization, and suggests that its expression is regulated post-transcriptionally.

본 연구에서는 4대강 살리기 사업 후 퇴적현상이 지배적으로 발생하는 남한강과 섬강 합류부 구간을 대상으로 2차원 수치모형인 CCHE2D 모형을 이용하여 하천의 흐름 및 하상변동에 대한 해석을 수행하였다. 대상지점 합류부는 남한강 본류의 만곡부에 지류 섬강이 유입되는 특성을 갖는다. CCHE2D 모형은 비평형 유사이송을 해석하며 소류사와 부유사 조정거리가 중요한 입력변수로 대상지점에서는 소류사 조정거리가 하상변동에 가장 큰 영향을 주는 것으로 나타났다. 수치모의 결과 유량비(Qr) 변화가 남한강과 섬강 합류부 지점에서 흐름 및 하상변동에 영향을 미쳤으며, Qr≤ 2.5인 경우에는 합류전 본류의 유속이 증가하여 흐름박리구역을 감소시켰으며 이로 인해 합류부 내측의 퇴적이 감소하였다. Qr>2.5이면 합류부 구간에 퇴적이 증가하여 사주가 형성될 가능성이 높은 것으로 나타났다. 수치모의를 통해 2013년에 발생한 유량비 변화에 의해 합류부에 고정사 주가 형성된 것을 알 수 있었다.

본 연구에서는 순환신경망을 이용한 댐 유입량 예측모형의 적용성 검토를 목적으로 하고 있으며, 이를 위해 소양강댐 유역 및 충주댐 유역을 대상 으로 그간 댐 운영을 통해 축적된 기상 및 수문 빅데이터를 활용하여 인공신경망 모형과 엘만 순환신경망 모형을 구축하였다. 모형의 학습과 예측 을 위하여 유역별 유입량, 강우량, 기온, 일조시간, 풍속자료가 입력자료로 사용되었고 10일간 일별 댐유입량 자료가 모델의 출력자료로 구조화 하여 학습을 진행한 후 검증을 목적으로 2016년 7월 ~ 2018년 6월까지 2개년에 대한 댐 유입량 예측을 수행하였다. 학습된 모형의 유입량 예측 결과를 비교분석한 결과, 소양강댐 유역에서는 인공신경망 모형과 순환신경망 모형 간 예측성능은 큰 차이를 보이지 않았으며, 충주댐 유역에서는 순환신경망 모형의 예측 결과가 인공신경망 모형에 비해 비교적 우수한 성능을 보임에 따라 엘만 순환신경망을 이용하여 댐 유입량 예측모형을 구축 할 경우 예측성능은 기존의 인공신경망 모형과 비슷하거나 다소 우수할 것으로 판단된다. 또한 엘만 순환신경망은 갈수기 댐 유입량 예측에 있어서 인공신경망에 비해 예측결과의 재현성이 우수한 것으로 나타났으며, 엘만 순환신경망 학습에 있어 다중 은닉층 구조가 단일 은닉층 구조보다 예측 성능 향상에 효과적인 것으로 분석되었다.

Safflower (Carthamus tinctorius L.) seeds have long been clinically used in Korea to promote bone formation and prevent osteoporosis. In addition, the safflower buds (SB) were found to have more useful functional ingredients than safflower seed. Thus, we investigated the preventive effects of SB diet in ovariectomized (OVX) rats. The rats were divided into five groups; sham operated group, OVX alone group, OVX plus 17β-estradiol (E2 10 ㎍/㎏, i.p.) and OVX plus SB diet feeding group (0.3% or 1%). Feeding of SB diet (0.3% or 3%) to OVX rats markedly increased trabecular formation in femur compared to OVX rats. Feeding of SB diet (0.3% or 3%) to OVX rats also decreased TRAP activity compared to OVX rats. These results suggest that SB diets have bone sparing effects by the decrease of osteoclast activity. We also observed that OVX rats fed with SB diet (0.3% or 3%) exhibited the decrease of calcium and phosphorus in serum compared to OVX-induced rats. Therefore, SB may be beneficial for the patients of osteoporosis, especially in postmenopausal women.

The pollen grain is a unique tricellular structure suitable for the delivery of the sperm cells to the ovule. All nutrients required for microspore and pollen cell growth are derived by passage through the anther locule and secretion by the tapetum lining. During later stages the tapetum degenerates but contributes to produce pigments, waxes, lipids and proteins which form the pollen coat and function in signaling between male (pollen) and female (pistil) tissues. The development of both normal pollen and tapetum is necessary for the fertilization processes in rice and would be exploited for the induction of male-sterility which is very useful to improve economic value of crops. We aredeveloping new approaches using a conditional male-sterility for the F1 hybrid seed production in rice. The conventional three parental systems for F1 hybrid seed production requirethe following three lines: male-sterile line, maintainer line, and restorer line. In this system, a critical requirement is to maintain the male-sterile inbred lines. Here we suggest molecular approaches, in which the engineered male-sterile plants are generated by regulating endogenous hormonal balance through the loss-of-function of genes. We can expect the male-sterility can be restored by exogenous applications of hormones such as gibberellin or jasmonic acid. Based on two parental systems, we will address the answer onfollowing question: how can we maintain a male-sterile line producing 100% male-sterile progenies without a maintainer? This work was supported by grants from Crop Functional Genomics Center of the 21C Frontier Program (CG1517), RepublicKorea.