We performed taxonomic reviews and habitat circumstances survey for 41 un-introduced environmentally harmful plants (as designated by the Ministry of Environment, Korea in 2016). For this purpose, we investigated plant specimens from several herbaria and performed a field survey in the southeastern region of the United States. Base on the result, we presented the most comprehensive results of weed risk evaluation and taxonomic description up to now as well as classification keys for 11 species to apply the regulation management of putative invasive alien species – Centaurea maculosa Lam., Centaurea diffusa Lam., Mikania micrantha Kunth. (Asteraceae), Cenchrus echinatus L., Neyraudia reynaudiana (Kunth) Keng ex Hitchcock, Brachiaria mutica (Forsskål) Stapf, Vulpia bromoides (L.) Gray, Lolium persicum Boissier & Hohennacker, Setaria palmifolia (J. Konig) Stapf (Poaceae), Prosopis glandulosa Torr. (Fabaceae), Fallopia baldschuanica (Regel) Hobub. (Polygonaceae).

2016년 환경부가 한반도 미유입 환경위해우려종으로 고시한 41분류군을 대상으로, 분류학적 특징을 이용하여 종을 동정하 는 방법을 제공하고 또한 자생지의 분포 및 환경 특성에 대한 정보를 제공하기 위하여 본 연구를 수행하였다. 이를 위하여 국외의 여러 표본관에 소장된 건조표본을 확인하였으며, 미국 서부 지역인 캘리포니아주, 오레곤주 및 워싱턴주의 현장조사를 수행하였다. 우선, 조사 결과의 일부로 미국 서부 농업지역을 중심으로 분포하는 Carduus acanthoides L. (국화과), Carduus tenuiflorus W. Curtis (국화과), Onopordum acanthium L. (국화과), Chromolaena odorata (L.) R.M. King & H. Rob. (국화과), Hydrocotyle ranunculoides L.f. (산형과), Oenanthe pimpinelloides L. (산형과), Ehrharta erecta Lam. (벼과), Paspalum conjugatum P.J. Bergius (벼과)의 8종에 대하여 위해성 검토, 잡초위험평가, 분류학적 특징 및 국내외 유사종과의 비교 검색표를 제시하였다.

본 연구는 경기도 파주시에 위치한 파평산의 관속식물상 조사를 위하여 수행하였으며, 이를 토대로 고유종, 희귀 및 멸종식물, 적색식물, 기후변화적응대상 식물, 귀화식물을 조사하였다. 2016년 4월부터 2019년 6월까지 총 13회에 걸쳐 현지조사를 실시한 결과 91과 257속 361종 9아종 36변종 4품종으로 총 410분류군이 확인되었다. 과별로 보면 국화과(46분류군), 벼과(31분류군), 장미과(27분류군), 콩과(18분류군) 순으로 높게 나타난다. 이 중 Chung 등이 지정한 특산식물은 9분류군, 환경부가 지정한 고유종은 6분류군이다. 희귀 및 멸종위기 식물은 IUCN 평가기준을 근거로 국립수목원이 지정한 멸종위기식물 13분류군이, 환경부가 지정한 적색자료집을 근거로 2분류군이 확인된다. 식물구계학적 특정식물은 Ⅳ등급 2분류군, Ⅲ등급 13분류군, Ⅱ등급 11분류군, Ⅰ등급 20분류군으로 총 46분류군이다. Lee (1976)에 따른 자원식물은 식용 182분류군(44.39%), 목초용 166분류군(40.49%), 약용 138분류군(33.65%), 관상용 53분류군(12.93%), 목재용 22분류군(5.37%), 다용도 11분류군(2.68%), 섬유용 6분류군(1.46%), 산업용 1분류군(0.24%) 순이며, 산림청 지정 자원식물은 식용 293분류군(71.46%), 산업용 186분류군(45.37%), 다용도 173분류군(42.20%), 의약용 125분류군(30.48%), 유해성 식물 84분류군(20.49%), 향료 16분류군(3.9%)순이다. 기후변화 적응대상식물은 7분류군이 확인되었으며, 귀화식물은 27분류군으로 귀화율 6.58%, 도시화지수 8.43%를 나타내었다. 본 파평산 일대의 조사로 본 결과는 파주 북부의 DMZ 접경지역의 자연환경 및 자연자원의 조사 및 보존에 필요한 기초자료로 활용될 수 있을 것으로 판단된다. 한편, 증거표본을 기준으로 하여 관속식물 목록을 작성하였기 때문에 조사과정에서 누락되었을 가능성을 고려하면 파평산의 관속식물의 다양성은 더 높아질 수 있을 것이다. 이러한 조사 연구를 통해 최근 높은 빈도로 발생하는 산불의 예방, 인위적 간섭에 의한 외래식물의 침입 및 산림훼손의 방지 등을 더 정확히 파악하고, 그를 활용할 더욱 적극적이고 다양한 노력이 필요할 것이다.

This study aims to investigate the flora of Mt. Cheonma (Namyangju-si, Gyeonggi-do, Korea), including Korean endemic, rare and endangered plants. To support the biodiversity and usefulness of this investigation, we checked the red list plants, floristic regional indicator plants, climate change adaptation target plants and naturalized plants, and compared composition of plant species in Mt. Cheonma with nearby mountains. Totally, we performed 22 field investigations from April 2012 to October 2014. The number of vascular plants in this region is 433 taxa and comprised of 94 families, 266 genera, 378 species, 2 subspecies, 46 varieties, and 7 forms. Among them, there are 13 endemic plants designated by Korean Forest Service, whereas only 10 taxa are endemic species by Korean Ministry of Environment (KME). According to the IUCN criteria, there are 12 rare and endangered plants composed of 9 Least Concerned (LC), 2 Vulnerable species (VU), and one Endangered species (EN) found by Korea National Arboretum (KNA). Meanwhile, 8 taxa including 7 Least Concerned (LC) were found in Red List of KME. The floristic regional indicator plants designated by KME were 49 taxa. In addition, there are 14 taxa in The Adaptable to Climate Change Plant List designated by KNA, while only 19 taxa are naturalized plants. Generally, there are 190 common taxa in Gwangju Mountains area, and only 51 taxa are distributed in Mt. Cheonma.

Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.

We performed the floristic study of Carpathian mountains in Ukraine from April 2008 till October 2009. We designated eight important areas to investigate in Ukraine Carpathians including Svydovets, Chornohora, Kuziy-Trybushany, Maramorosh, Uholka-Shyrokyi Luh, Narcissi Valley, Chorna Hora, and Yulivska Hora. The total numbers of vascular plants in investigated area were 1,349 speices from 502 genera and 112 families. The endemic species to Carpathians numbered to 65 from 49 genera belonging to 24 families, and it was corresponded to 4.8% of the 1,349 vascular species. We prepared the list of vascular plants with compiling database on useful plants. Ukraine Carpathians endemic species could be applied as high value products in research and practice. Moreover, the flora of Carpathian mountains which is distributed in five nations in eastern Europe and famous for Primeval Beech Forests (Fagus sylvatica), is useful to compare the floristic study with Korean flora.

Randomly Amplified Polymorphic DNA (RAPD) was performed to define the genetic variation and relationships of Artemisia capillaris. Fifteen populations by the distributions and habitat were collected to conduct RAPD analysis. RAPD markers were observed mainly between 300bp and 1600bp. Total 72 scorable markers from 7 primers were applied to generate the genetic matrix, and 69 bands were polymorphic and only 3 bands were monomorphic. The genetic dissimilarity matrix by Nei's genetic distance (1972) and UPGMA phenogram were produced from the data matrix. Populations of Artemisia capillaris were clustered with high genetic affinities and cluster patterns were correlated with distributional patterns. Two big groups were clustered as southern area group and middle area group. The closest OTUs were GW2 and GG1 in middle area group, and GB1 from southern area group was clustered with OTUs in middle area group. RAPD data was useful to define the genetic variations and relationships of A. capillaris.

RAPD analysis was performed to discuss the taxonomic status and phylogenetic relationships among the tribe Forsythieae and related groups. Two hundred and eighteen scorable polymorphic bands were detected from fourteen oligonucleotide primers. From the results of RAPD analysis by Nei and Li's genetic distance, each individuals of Abeliophyllum distichum showed high genetic relationships with ranging from 0.085 to 0.301, also the genus Forsythia showed from 0.042 to 0.655 among the species and populations. But, Abeliophyllum and Forsythia showed distinct dissimilarity, ranging from 0.610 to 1.258. And genetic differences among the population of Forsythia were 0.042 in F. koreana, 0.275 in F. saxatilis, 0.275 in F. ovata, 0.279 in F. nakaii, and 0.249 in F. viridissima. The UPGMA phenogram of tribe Forsythieae based on the results of RAPD analysis were presented that Abeliophyllum is distinct genus different from Forsythia. NJ tree which applied as the outgroups Fontanesia and Jasminum was derived, and it showed that tribe Forsythieae might be a monophyletic group. The genus Fontanesia was showed as sister group of tribe Forsythieae. Among the populations of taxa in Forsythia, F. koreana and F. saxatilis were more closely related, and F. ovata and F. nakaii were very closely related to F.japonica. And Fontanesia was the sister group of tribe Forsythieae.

Fruit-discharge type of forty species of Asian Geranium were examined and their evolutionary trends and taxonomic system were discussed. Two main types of seed discharge in Geranium are made the basis of its divison into subgenera: Geranium subgenus Geranium, with a ballistic expulsion of the seed from mericarp, Geranium subgenus Robertium, with forcible discharge of the mericarp with the seed in it, seperately from the awn. Variants of the seed ejecting type permit the division of Geranium subgenus Geranium into three sections. Section Geranium, these has bristles at the base of the mericarp for prevent the seed from dropping from the divergent mericarp. Section Dissect, these has prongs at the base of the mericarp for prevent the seed from dropping from the divergent mericarp. Section Tuberosa, these has no bristles and prongs at the base of the mericarp, instead, retained of seed in pre-explosive interval by twist. Geranium subgenus Robertium was divided into two sections, Section Ruberta, with deeply wrinkled on the surface of mericarp at the time of discharge. Section Batrachioides, and section Trilopha, without wrinkle on the surface of mericarp at the time of discharge. According to the seed discharge, subgenus Geranium is thought to be more advanced than subgenus Robertium.

This study characterized the genetic variations of 13 populations of Asplenium antiquum and its relative species using randomly amplified polymorphic DNA (RAPD) markers. A total 88 scorable RAPD bands were generated by the 12 random oligo primers and were analyzed by Nei and Li's genetic distance. High genetic variability was detected between A. antiquum and A. nidus, with the range from 0.568 to 0.682. And slightly low genetic variations showed within the populations of same species. Seven populations of A. antiquum showed slight differences (0.000-0.216), and five populations of A. nidus showed similar low genetic variations (0.114 to 0.171). Two individuals from Sup-seom Island which are growing in might be the regenerated one from abroad. A. antiquum were clustered as two groups (Group I, Group II) by UPGMA phenogram. And five populations of A. nidus were clustered as two groups correlated with geographical distribution. The RAPD data was very useful to define the genetic variations and to discuss the phylogenetic relationships among A. antiquum and the related species..