Radioisotope ADME (RI-ADME) studies are enabling visualization of the biodistribution in molecular imaging. We applied RI-ADME to investigate the tumor targeting capacity and biodistribution of trastuzumab-monomethyl auristatin F (LCB14-0110) in JIMT-1 xenograft mice and healthy marmoset. The LCB14-0110 was labelled with 125I. 125I-LCB14-0110 was intravenously administered to the animals. The gamma-count and single-photon emission computed tomography/computed tomography (SPECT/CT) was conducted for biodistributioon and bioimaging of the biopharmaceutics. Tumor uptake in xenograft mice was highest at three-day after 125I-LCB14-0110 administration in both the biodistribution and SPECT/CT bioimaging. Alternatively, blood and organ tissues showed gradual decrease in radioactivity over time. In marmosets, radioactivity in all organ tissues rapidly reduced and no specific targeting of organs was observed in the biodistribution study and SPECT/CT imaging. Hence, 125ILCB14- 0110 demonstrated effective tumor targeting capacity and accumulated in JIMT-1 cell-bearing mice. However, accumulation did not occur in the organs of xenograft mice. Additionally, marmosets showed rapidly decrease in radioactivity throughout the entire body without accumulation in the normal organs. We also confirmed that the drug distribution was similar in normal organs between the two experimental animal species except spleen. Therefore, 125I is expected to be a useful tool in the study of RI-ADME in biopharmaceuticals through minimal antibody modification.

The Semi-Rigid Pavement (SRP) mixture is composed of Gap Graded Asphalt (GGA) mixture (air void = 20~28%) and cement paste. By inserting cement paste into voids in GGA mixture, SRP can provide not only flexibility but also rigidity characteristics on pavement performance. SRP can mitigate pavement surface temperature increase during summer session, provide better smoothness and mitigate rutting distress due to heavy weight vehicles, successfully. In Japan, SRP is widely applied in cross section area, heavy vehicle parking lot and highway ticketing booth in highway network system. In South Korea, SRP was introduced and applied since 2005. However, still more researches and studies are needed to understand material characteristics and improve performance of SRP. Moreover, the current SRP system in South Korea merely follows and adapts the aggregate gradation information from Japan which needs to be amended and customized into original material (i.e. aggregate, binder and cement) situation of South Korea. In this paper, SRP system based on Stone Mastic Asphalt (SMA) mixture design originated from Korea Expressway Corporation (KEC) and enhanced cement paste with addition of fly-ash and slags was developed. In addition, an optimized proportion between asphalt mixture air voids and cement paste amount with consideration of economic benefit was introduced. Based on field evaluation process it can be said that the newly developed SRP system can successfully adapted not only in static site on highway: parking lots or ticketing booth, but also in dynamic site on highway: driving and wheel path.

True hermaphrodites are animals of equivocal sex in which both male and female gonads develop simultaneously. The frequency of true hermaphroditism is higher in pigs than in other domestic animals. Two Korean pigs were diagnosed with true hermaphroditism showing ovotestes, epididymes, penes, and uteri. Histomorphologically, the testicular tissues consisted of Sertoli cells that were devoid of spermatogenic germ cells and showed proliferation of interstitial cells. However, the uteri were of normal architecture and had well-developed uterine endometrial glands. The samples were 38, XX female karyotype without the sex-determining region Y (SRY) gene. The findings of this study could contribute to the understanding of true hermaphroditism in the Korean pig industry. * This work was supported by a grant (Code# PJ008148) from BioGreen21 Program, Rural Development Administration, Republic of Korea.

Recently, farmers of mushroom farms in Korea express the difficulty of shiitake cultivation due to the damage of oak log beds by unknown reason. To elucidate the cause of the damage, we investigated and isolated fungi from inside wood chip of the damaged shiitake log bed. Four of green fungi were obtained and their morphology and molecular properties examined. Trichoderma atroviride, Trichoderma citrinoviride, Hypocrea lixii (Trichoderma harzianum), and Hypocrea lutea (Gliocladiumviride) were identified. Hypocarea lutea is newly described in Korea. These species were able to produce extracellular enzymes such as cellulase, pectinase, and xylanse that might degrade wood components of oak logs. This study provides strong evidence that Trichoderma fungi are involved in the recent damages of shiitake log beds In Korea.