In Sri Lanka, the shoulder in asphalt pavements has been constructed using the materials transported from borrow pit in the iRoad Project due to the low quality of in-situ soils. After excavating 150~200mm thick and 500mm wide shoulder area, the borrow pit materials are placed and compacted according to specifications. The excavated in-situ soils are dumped in designated location. It is estimated that this process of shoulder construction is not economical due to high material transportation cost and can also induce the environmental issues by disposal of in-situ soils. It can also cause distresses such as surface rutting and edge drop-off in soft shoulder section due to bearing capacity failure and off-tracking of vehicle. The heavy rainfall in Sri Lanka can induce severe erosion problem when using the soft shoulder. To improve the strength and durability of pavement shoulders in the iRoad Project, the soil stabilization will be a good alternative to solve the above mentioned problems. The use of in-situ soils with addition of soil stabilizer enables to reduce the construction cost of shoulder section and mitigate the environment issues. The objective of this task is to review the application of soil stabilization method for soft shoulder construction in the iRoad Project. Firstly, the quantitative analysis of soil strength improvement due to soil stabilization was done for soil samples collected from iRoad construction sites. Two types of soils were selected from iRoad Project sites and prepared for soil stabilization testing by the Road Development Authority. Secondly, the appropriate stabilizer was selected at given soil type based on test results. Three different stabilizers, ST-1, ST-2, and ST-3, produced in Korea were used for estimating soil strength improvements. Finally, the optimum stabilizer content was determined for improving shoulder performance. The uniaxial compressive strength (UCS) test was conducted to evaluate the strength of stabilized soil samples in accordance with ASTM D 1633. The use of bottom ash as a stabilizer produced from power plant in Sri Lanka was also reviewed in this task.

Nitrogen (N) fertilization is essential for alleviating nutrient deficiencies of the world’s population by increasing rice production, one of the most important food crops of our time. Here we established an in vivo hydroponics rice seedling culture system to investigate the physio-biochemical and molecular responses of various rice genotypes to low nitrogen application. Yoshida’s nutrient solution (YS) was used to grow rice seedlings, and at three-week-old the seedlings manifested highly stable and reproducible symptoms, such as reduced shoot growth and length. Out of 12 genetically selected or tested genotypes, almost all (11 genotypes) showed varied degrees of growth reduction response to applied nitrogen (4 and 40 ppm N for treatment and control, respectively), but SR19663-B-B-34-3-3-3-1 showed similar growth as the control though its leaf width was smaller than the control. The leaves of a 11 representative low nitrogen-responsive genotype as BG90-2 were sampled for revealing the protein profiles between low and normal (control) nitrogen application by using two-dimensional gel electrophoresis (2-DGE) followed by staining of separated proteins with silver. Fifty differentially expressed silver stained protein spots were excised from 2-D gels and 41 proteins identified using high-throughput mass spectrometry (MS) using matrix-assisted laser desorption/ionization-time of flight-MS and nano electrospray ionization liquid chromatography tandem MS. These proteins could be assigned as major (energy metabolism, photosynthesis and oxidative stress) and minor functional categories, revealing many novel low N-responsive proteins, including those having energy/photosynthesis, and defense/stress, and iron homeostasis-related functions.