Offspring have been produced from somatic cells in a number of species. This biotechnology introduced a new phenomenon in reprogramming and differentiation of somatic cell, namely totipotency. However, birth of oversized calves and perinatal abnormalities such as increased gestation length, lack of spontaneous parturition, higher incidence of dystocia, and reduced perinatal viability of offspring are frequently observed in pregnancies of cloned bovine fetuses. Disturbance of feto-placenta has been proposed as likely causes for abnomal growth. However. Little is known the mechanism responsible for the perinatal problems. Therefore, we focused on gestation length in somatic cell nuclear recipient cows. To solve this issues, placental tissues of control and cloned bovine were obtained by a cesarean section (C-section) before 5 days of paturition. Total RNA from control and cloned bovine placenta was extractd by TRIzol reagent. GeneFishing DEG kits (Seegene) were used to identify differentially expression genes. Total RNA (3 ug) were synthesized by M-MLV reverse transcriptase (200 u/ul) with 10 uM dT-annealing control primer (ACP1) at 42C for 90 min. Then, first-strand cDNA (50 ng) was amplified using the 5 uM arbitary ACP (1-20) and 10 uM dT-ACP2 primers. Some specific expression genes were amplified, Now, we are cloning and sequencing. These finding strongly can be support to solve the problems for parturition delay in nuclear transfer cows, suggest that placenta specific proteins are key indicators for the aberration of gestation and placental function in cows.

간척지 제염정도에서 유실되는 유효한 비료성분을 흡착하고 완급하여 비효를 증진시키기 위하여 토양개량제인 Zeolite를 시용하여 수도 생육과 그 수량에 미치는 영향을 시험한 결과를 요약하면 다음과 같다. 1) Zeolite를 시용한 처리구에서의 토양염도 변화는 최고분얼기 이후에 무시용구에 비해 0.07∼0.12%차로 감소되었다. 2) 수도생육에 있어서 Zeolite시용 효과는 각 구 공히 Zeolite 600kg시용구가 양호하였으며 그 효과는 최고분얼기에 현저히 나타나 수량까지 영향을 미쳤다. 3) Zeolite시용구에 있어서 수수 및 일수영화수는 증수요인으로 작용하였고, 유효경율과 등숙비율은 수량과 부의 경향이었다. 4) 현미수량에 있어서 Zeolite시용 효과는 인도형인 '유신'이 일본형인 '진흥'보다 현저하게 나타났다. 5) Zeolite시용에 있어서 양 시비(표준비구와 1.5신 비구 및 품종(유신과 진흥) 처리구 공히 600kg/10a가 적정량으로 인정되었다.