To preserve the superior genetic resources and restore the endangered species, Somatic cell nuclear transfer (SCNT) has been used widely. In Korea, the research of dog cloning has made outstanding achievements including the production of the world`s first cloned dog. Sapsaree (Sapsalgae), the representative dog of Gyeongsan-si was designated as a Korea natural monument (No. 368). This male dog used in this study has azoospermia due to unknown cause. In this study, the aim was to confirm the cause of infertility in the cell donor dog and to evaluate the reproduction potential of dog cloning using infertile male dog by SCNT. First, to confirm the infertility of the cell donor dog, the reproductive history and the testis were evaluated. The breeding histology was not recorded in individual document. In histopathology, the Sertoli cell tumor was confirmed in biopsy of the cell donor dog after death. But, these tumors are predominantly in older dogs. Second, we produced the cloned dogs with the somatic cells of the infertile dog and the appearance was similar with the cell donor dog. Also, microsatellite analysis confirmed the genetic relationship between the cell donor and clone dogs. Third, the potential breeding capacity of the cloned dog was confirmed. In T4 assay, the normal dog (same age with cloned dogs), cell donor dog, and cloned dogs was investigated. The cell donor dog with azoospermia had very low T4 level, and cloned dogs showed higher level of T4 than normal dogs. In CASA, There was no significant difference in sperm motor ability between normal dogs and cloned dogs. As a result, cloned dogs produced by SCNT had no problem regarding the reproductive function of the testis. In AI experiment, the semen of clone dogs was used to fertilize a natural female bitch and was diagnosed pregnancy by ultrasonography. In total, 7 puppies were born by normal delivery (male: 3, female: 4). In conclusion, this study confirmed that the reproduction problem of non-genetic infertility can generate a normal descendant by SCNT. Also, the first successful research to restore infertile dogs was completed. Furthermore, SCNT would be useful for the restoration of endangered species and application of superior traits.

The performance of grass filter strips (GFS) in abating P04-P concentrations from the forage cropland was tested in an experiment on the 10% slope in Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration (RDA) from October 2007 to September 2009. Forage croplands with rye-corn double cropping system applied with chemical fertilizer and livestock manure (LM) were compared in a natural condition. The plots were hydrologically isolated Main plots consisted of the length of GFS, such as Om, 5m, 10m and 15m. Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). Concentrations of P04-P in surface runoff water were reduced as the length of GFS increased. Especially, GFS with 10m and 15m reduced PO4-P concentrations significantly compared to that with Om and 5m (p<0.05). The results from this study suggest that GFS improved the removal and trapping PO4-P from forage croplands.

답잡초 돌피 외 19종을 대상으로 병원균군, 접종방법 및 잡율의 생육시기를 달리하여 벼흰빛잎마름병의 기주범국 구명시험에서 얻어진 결과를 요약하면 다음과 같다. 1. 나도겨풀, 강아지풀, 독새풀 등은 밀양 2003에 준한 이병성을 나타냈으며, 그 밖에 바랭이, 왕바램이 알방동산이, 너도방동산이, 바람하늘지기 등에서 이병성을 보였으나 광엽잡초에는 전혀 이병되지 않았다. 2. 생육초기에 이병성을 크게 보인 강아지풀, 나도겨풀, 독새풀은 생육전기간을 통해서 이병성이 컸으며, 균군간에는 제일군이 병원성이 다소 큰 것 같았다. 3. 접종방법별로는 유상접종인 핀 및 가위 접종이 무상접종의 분무접종보다 이병성이 켰다.