Background: Somatic cell nuclear transfer (SCNT) is a prominent technology that can preserve superior genetic traits of animals and expand the population in a short time. Hematological characters and endocrine profiles are important elements that demonstrate the stability of the physiological state of cloned animals. To date, several studies regarding cloned camels with superior genes have been conducted. However, detailed hemato-physiological assessments to prove that cloned camels are physiologically normal are limited. In this study, We evaluated the hemato-physiological characteristics of cloned male and female dromedary camels (Camelus dromedaries). Methods: Therefore, we analyzed variations in hematological characteristics and endocrine profiles between cloned and non-cloned age-matched male and female dromedary camels (Camelus dromedaries ). Two groups each of male and female cloned and non-cloned camels were monitored to investigate the differences in hemato-physiological characteristics. Results: All the animals were evaluated by performing complete blood count (CBC), serum chemistry, and endocrinological tests. We found no significant difference between the cloned and non-cloned camels. Furthermore, the blood chemistry and endocrine profiles in male and female camels before maturity were similar. Conclusions: These results suggest that cloned and non-cloned camels have similar hematological characteristics and endocrine parameters.

Background: Canine induced pluripotent stem cells (iPSCs) are an attractive source for veterinary regenerative medicine, disease modeling, and drug development. Here we used vitamin C (Vc) to improve the reprogramming efficiency of canine iPSCs, and its functions in the reprogramming process were elucidated. Methods: Retroviral transduction of Oct4, Sox2, Klf4, c-Myc (OSKM), and GFP was employed to induce reprogramming in canine fetal fibroblasts. Following transduction, the culture medium was subsequently replaced with ESC medium containing Vc to determine the effect on reprogramming activity. Results: The number of AP-positive iPSC colonies dramatically increased in culture conditions supplemented with Vc. Vc enhanced the efficacy of retrovirus transduction, which appears to be correlated with enhanced cell proliferation capacity. To confirm the characteristics of the Vc-treated iPSCs, the cells were cultured to passage 5, and pluripotency markers including Oct4, Sox2, Nanog, and Tra-1-60 were observed by immunocytochemistry. The expression of endogenous pluripotent genes (Oct4, Nanog, Rex1, and telomerase) were also verified by PCR. The complete silencing of exogenously transduced human OSKM factors was observed exclusively in canine iPSCs treated with Vc. Canine iPSCs treated with Vc are capable of forming embryoid bodies in vitro and have spontaneously differentiated into three germ layers. Conclusions: Our findings emphasize a straightforward method for enhancing the efficiency of canine iPSC generation and provide insight into the Vc effect on the reprogramming process.

Efficiently detecting the nearest navigational dangers in Electronic Chart Display and Information Systems (ECDIS) remains pivotal for maritime safety. However, the software implementation of ADMAR(Automatic Distance measurement and Ranging) functionality faced challenges, necessitating extensive computations across ENC cells and impacting real-time performance. To address this, we present a novel method employing dynamic programming. Our proposed algorithm strategically organizes nodes into a tree structure, optimizing the search process towards nodes likely to contain navigational hazards. Implementation of this method resulted in a notable sevenfold reduction in computation time compared to the conventional Brute Force approach. Our study established a direct correlation between the ADMAR functionality and node count, achieving error margins deemed acceptable for practical navigation scenarios. Despite this theoretical progress, minor errors in results prompt further refinement. Consequently, future iterations will explore varying values for N, considering hierarchy and cell sizes to enhance algorithmic precision. This research signifies a potential advancement in optimizing navigational danger detection within ECDIS, offering a promising avenue for improved efficiency. By introducing a dynamic programming-based approach, we have streamlined the detection process while acknowledging the scope for algorithmic refinement in subsequent studies. Our findings underline the feasibility of employing dynamic programming to enhance navigational danger detection, emphasizing its potential in ensuring maritime safety. This work lays a foundation for future research endeavors, aiming to fine-tune algorithms and advance navigational safety measures in ECDIS.

This study analyzed the relationship between efficient pitchers and teams advancing to the postseason in Korean professional baseball through DEA. A total of 1,133 pitchers who threw more than one inning from the 2014 season to the 2018 season were selected for this study. For DEA analysis, input variables were selected as annual salary and inning output variables as Wins, Saves, and Holds and the number of efficient pitchers for each season was classified using the input-oriented BCC model. After that, it was divided into two groups based on joining the postseason or not, and the number of efficient pitchers was compared through a prop test. As a result of the analysis, the groups that advanced to the postseason in the rest of the season except for the 2014 and 2017 seasons had more efficient pitchers. Considering that the 2014 season recorded the highest WAR (Wins Above Replacement) at 183.56 compared to other seasons, most pitchers threw well, and in the 2017 season, they made more mistakes in pitching than in other seasons, but they performed well in batters. The results of this study have expanded the research field using efficiency analysis in professional baseball and can be used as useful data for practical research.

This study was conducted at the National Institute of Animal Science (NIAS) from 2010 to 2017 to develop a late-maturing variety with high productivity for cultivation in the southern region of South Korea. The new variety of Italian ryegrass, ‘IR901’, was a late-maturing variety, and its heading date was 22 May, 17 days later than that of the control variety ‘Kowinearly’. ‘IR901’ had a flag leaf width of 11.2 mm, flag leaf length of 31.8 cm, and plant length of 103 cm on its heading date. The combined average dry matter yield of ‘IR901’ in all three adaptability evaluation regions (Cheonan, Pyeongchang and Haenam) was 7,747 kg/ha, which was similar to that of the ‘Kowinearly’ variety (7,734 kg/ ha). However, the average dry matter yield over three years in Cheonan and Pyeongchang was 82% and 96%, respectively, compared to that of the control, which was most likely because of the poor cold tolerance of ‘IR901’. By contrast, in Haenam, in the southern region, the average dry matter yield of ‘IR901’ was 19% higher than that of the ‘Kowinearly’ variety. The proportions of crude protein (CP), total digestible nutrients (TDN), acid detergent fiber (ADF), and neutral detergent fiber (NDF) in ‘IR901’ were 8.6%, 59.7%, 36.9%, and 54.8%, respectively; the proportions were 0.2% lower, the same, the same, and 2.5% lower than those in the ‘Kowinearly’ variety. The determined in vitro dry matter digestibility (IVDMD) of ‘IR901’ was 72.2% higher than that of ‘Kowinearly’ (67.2). In general, of the two varieties, the forage quality of ‘IR901’ was marginally superior to that of ‘Kowinearly’.

This study conducted a survey and evaluation on ‘The 2021 Jeju World Natural Heritage Festival’ with a framework of various stakeholders related to festivals. While aiming for the essential context of the celebration, we tried to find suggestions to ensure the continuity of the celebration and the designation of the World Natural Heritage. As a methodology, surveys and interview surveys were conducted in parallel with the framework of multifaceted evaluation. To summarize the important contents presented as policy suggestions, first, it is necessary to expand the concept of ' encounter with hidden nature' from the positivity of the concept of 'encounter with hidden nature' to have fellowship with mankind about natural heritage. For reference to this, efforts should be made to secure the continuity of the designation of World Natural Heritage through a contextual approach using the frame of 'encounter'. Second, since the participation of experts can help in many ways, it is necessary to encourage many experts to participate as a staff or activist. Third, it is necessary to sublimate Jeju's natural heritage into a public celebration without damaging it. Finally, it should be a festival centered on local communities and local residents. It is necessary to actively seek a system in which the World Natural Heritage Village Preservation Association, which will be created (tentative name), will be centered and promoted by the villagers.

As the low birth rate and aging phenomenon intensify, many local governments are facing a difficult situation of not only reducing population but also for securing financial resources. The national average fiscal independence is not increasing, and the fiscal gap between local governments has not been resolved. Accordingly, the Hometown Love Donation System, which provides tax incentives for donations to local governments, was included in the current government's 100 major national tasks and roadmap for autonomous decentralization. The hometown tax system was introduced in Japan in 2008 and has been contributing to securing financial resources of local governments, and has been contributing to regional development in recent years by using local specialties as return products, but concerns about overheating of competition are also growing. In Korea, the National Assembly and local governments have continuously reviewed the introduction of the hometown tax system. In addition, it is proposed to organize and operate a research conference about the suitabity of the environment of Jeju in order to minimize trial and error in the introduction and implementation of the system based on the case of Japan's hometown tax, taking into account domestic and Jeju special selfgoverning conditions. In the future, further research is needed in policy making and related systems for the donation deduction system.

Ostrea denselamellosa and Eriocheir japonica samples were collected from the Seomjin River in 2019 as part of the “Research of Host-Associated Bacteria” research program. Almost 200 bacterial strains were isolated from the O. denselamellosa and E. japonica samples and subsequently identified by 16S rRNA gene sequencing. Among the bacterial isolates, ten strains possessed greater than 98.7% sequence similarity with published bacterial species that had not previously been recorded in Korea. These species were phylogenetically diverse, belonging to three phyla, four classes, seven orders, and eight genera. At the genus and class level, the previously unrecorded species belonged to Pseudoalteromonas, Aliivibrio, Rheinheimera, Leucothrix, and Shewanella of the class Gamma-proteobacteria, Olleya of the class Flavobacteriia, Algoriphagus of the class Cytophagia, and Lactococcus of the class Bacilli. The previously unrecorded species were further characterized by examining their Gram staining, colony and cell morphology, biochemical properties, and phylogenetic positions.

Background: Forward head posture (FHP) is a postural alignment of the cervical vertebrae that leads to increased gravitational load on cervical segmental motions. The overhead arm lift test assesses the ability to actively dissociate and control low cervical flexion and move the shoulders through overhead flexion. Objects: The purpose of this study was to explore muscle activities in the upper trapezius (UT), serratus anterior (SA), sternocleidomastoid (SCM), and lower trapezius (LT) alongside changes in head position during the overhead arm lift test in individuals with FHP. Methods: Fifteen subjects with forward head posture and fifteen subjects with normal subjcects were enrolled in this study. The patients performed the overhead arm lift test, and muscle activities of the UT, SCM, SA, and LT were measured using surface electromyography and by evaluating changes in head position. Independent t-tests were used to detect significant differences between the two groups and Cohen’s d was calculated to measure the size of the mean difference between the groups. Results: The FHP group demonstrated significantly increased muscle activity of the UT (32.46±7.64), SCM (12.79±4.01), and LT (45.65±10.52) and significantly decreased activity in the SA (26.65±6.15) than the normal group. The change in head position was significantly higher in the FHP group (6.66±2.08) than the normal group. Effect sizes for all parameters assessed were large between the two groups. Conclusion: The subjects with excessive FHP displayed were unable to fix their heads in position during the overhead arm lift test. The overhead arm lift test can thus be used in clinical settings to confirm control of the neck in these subjects.