The aim of this study was to determine the therapeutic effects of beauty care on negative mental health, including stress and depression. Nail care, massage care, and makeup were used as programs for beauty therapy. Qualitative research was conducted with six female participants over five-month period. After interviewing the subjects in advance, beauty care treatments were performed every week 4 weeks in the following order: nail care, massage, and makeup. The results are as follows, First, the participants perceived beauty care in the form of 4 concepts: “courtesy in social life,” “investment in oneself,” “self-satisfaction,” and “self-care.” Second, the effects of beauty therapy were categorized as “psychological effects,” “confidence,” “behavioral changes,” “evaluation of others,” and “positive social effects.” Third, each subject showed different psychological effects during the process when the function of the therapy took effect through the beauty care treatment. It was confirmed that confidence levels increased as a result of treatment through the process of becoming re-aware of oneself. Positive statements from the participants included: “I want to go out,” “I have become kind and positive to others,” “I have become more active in a given task,” and “It seems that my work ability is improving.” Finally, t-test results for selfesteem, depression, and stress showed there were significant differences in self-esteem and depression. This confirmed that self- esteem increased, and depression decreased after the beauty care treatment.

Control mating is important aspect in bee breeding programs. The technique of artificial insemination is the possible one that can surely control mating of the selected drones with the virgin queen. This is the first time applied artificial insemination technique to control mating of A. cerana in Korea. Altogether 18 queens were artificially inseminated, and 2,000 drones of Korean A. cerana were used to evaluate amount of semen collection. Semen of A. cerana is much difficult to separate from mucus in comparing with A. mellifera. The average amount of semen can be collected from one A. cerana drone was 0.09 μl, whereas the A. mellifera was more than 6 times (0.58 μl semen per A mellifera drone). Obtaining 1 μl of semen have to collect from 11.94 drones that successful semen ejection and have to kill 17 A. cerana drones. Queens artificially inseminated with 4 μl of semen (once insemination) or 8 μl of semen (twice insemination, each with 4 μl of semen) started laying egg later than naturally mated queens 5.3 and 2.5 days, respectively. The onsets of oviposition of artificially inseminated queens were 12.5 to 15.3 days. Queens received twice inseminations started laying eggs 2.8 days earlier than those received only once insemination. Artificially inseminated queens produced exclusively brood and were similar as the naturally mated ones. The brood production of the queens received once insemination with 4 μl of semen was insignificantly different than those received twice inseminations or naturally mated ones, suggesting that one artificial insemination with 4 μl of semen is favorable.

Due to their environmental and economical consequences, invasive species have become a major concern worldwide. Among them, the yellow-legged hornet, Vespa velutina, is a keen hunter of domestic honeybees. They use olfactory cues from the prey to assess food information. In this study, we investigated the attraction behavior of V. velutina to honeybee pheromone under outdoor conditions. Nine honeybee pheromones were tested and compared in order to find the best attractant. When testing specific compounds, the honeybee queen pheromone, homovanillyl alcohol, proved highly attractive.

Anthricin (Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer properties in several cancer cells, such as prostate cancer, cervical carcinoma and pancreatic cancer. However, the effects of Anthricin are currently unknown in oral cancer. We examined the anticancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner (IC50 50 nM) in the MTT assay and Live & Dead assay. In addition, Anthricin treated FaDu cells showed marked apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factors such as caspase-3, -9 and poly (ADP-ribose) polymerase (PARP), suggesting that caspase-mediated pathways are involved in Anthricin- induced apoptosis. Anthricin treatment also leads to accumulation of the pro-apoptotic factor Bax, followed by inhibition of cell growth. Taken together, these results indicate that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells is mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as a novel chemotherapeutic drug for human oral cancer.

Zika virus, mosquito-borne disease, caused by mosquitoes has been increased the importance. From March to September, twice a month from 7 different points (3 residential areas, 3 migratory bird sanctuary and 1 cattle shed) were collected using BG trap and BL trap. After identifying the mosquitoes collected, we confirmed the virus infection. Total 26,531 mosquitoes (6 genus 9 species) were collected, virus has been detected from the 3 species (Aedes vexans, Cuilex tritaeniorhynchus, and Amigeres subalbatus) of mosquitoes of them. It showed the highest peak in August, and then gradually decreased. The most common mosquito species was collected Aedes vexan (16,637) in the cattle shed.

The two spotted mite, Tetranychus urticae Koch, is a global pest, and has developed severe resistance to several types of acaricides. This study compared the development in susceptible (S) and acequinocyl, bifenazate, pyridaben, abamectin and etoxazole-resistant (AcR, BR, PR, AbR and ER) strains of T. urticae by X-ray irradiation. When eggs were irradiated with 100 Gy, the egg hatching was completely inhibited in all strains. When nymphs were irradiated with 200 Gy, their hatching was completely inhibited in all strains. When adults were irradiated, the fecundity and egg hatching was decreased at 150 Gy and above, and hatchability of F1 generation was completely inhibited at 300 Gy in all strains. Adult longevity was slightly increase at 150 Gy and above. In addition, we performed quantitative real-time PCR on several genes. To examine the difference of all strains on radiation stress-induced gene expression, we performed quantitative RT-PCR (qPCR) of several known stress-induced genes.

Opioid receptors have been pharmacologically classified as µ, δ, κ and ε. We have recently reported that the antinociceptive effect of morphine (a µ-opioid receptor agonist), but not that of β-endorphin (a novel µ/ε-opioid receptor agonist), is attenuated by whole body irradiation (WBI). It is unclear at present whether WBI has differential effects on the antinociceptive effects of µ-, δ-, κ- and ε-opioid receptor agonists. In our current experiments, male ICR mice were exposed to WBI (5Gy) from a 60 Co gamma-source and the antinociceptive effects of opioid receptor agonists were assessed two hours later using the hot water (52℃) tail-immersion test. Morphine and D-Ala2,N-Me-Phe4,Gly-ol-enkephalin (DAMGO), [D-Pen2-D-Pen5]enkephalin (DPDPE), trans-3,4-Dichloro-N-methyl-N-[2-(1-pyrrolidinyl)- cyclohexyl]¬benzeneacetamide (U50,488H), and β-endorphin were tested as agonists for µ, δ, κ, and ε-opioid receptors, respectively. WBI significantly attenuated the antinociceptive effects of morphine and DAMGO, but increased those of β-endorphin. The antinociceptive effects of DPDPE and U50,488H were not affected by WBI. In addition, to more preciously understand the differential effects of WBI on µ- and ε¬opioid receptor agonists, we assessed pretreatment effects of β-funaltrexamine (β-FNA, a µ-opioid receptor antagonist) or β-endorphin1-27 (β-EP1-27, an ε-opioid receptor antagonist), and found that pretreatment with β-FNA significantly attenuated the antinociceptive effects of morphine and β endorphin by WBI. significantly reversed the β-EP1-27 attenuation of morphine by WBI and significantly attenuated the increased effects of β-endorphin by WBI. The results demonstrate differential sensitivities of opioid receptors to WBI, especially for µ- and ε-opioid receptors.

본고에서는 중국시장에 상장된 A주 시장과 홍콩시장에 교차상장된 H주 시장 간의 가격정보 전달과정을 분석하고 동일한 주식의 두 시장 간의 가격 차이에 대한 기존 가설을 분석하였다. 또한 시장의 개방 전후 시점을 비교하여 본국시장주도가설과 정보비대칭가설도 검정하였다. 주가지수를 이용한 GJR-GARCH모형을 통한 분석에서 본국주도가설은 설득력이 떨어짐을 알 수 있었다. 변동성이전효과를 나타내는 계수 값은 외부 시장인 홍콩 H주 지수시장에서 중국 국내시장인 상해 및 심천으로 한쪽 방향으로만 통계적으로 유의함을 보여주었다. 즉, 외부 선진시장에서 본국시장으로 변동성 이전효과가 존재함을 보여주고 있다. 그러나 중국 시장 개방 이후 수익률의 정보 이전효과를 의미하는 계수 값이 큰 폭으로 증가하고 다른 계수는 서로 일방적인 영향이 없는 것으로 나타났다. 시장이 개방됨에 따라 홍콩과 중국의 두 시장은 거의 동시에 서로 영향을 미치는 통합된 시장으로 감을 알 수 있다. 양 시장에 교차 상장된 개별지수를 이용한 인과관계검정을 통한 분석에서는 두 시장주식들 간에 피드백 또는 상호인과관계가 존재한다고 보기는 어려웠다. 개방 후에는 외국인도 정보에 더 용이하게 접근할 수 있음에도 불구하고 개방후가 개방전보다 국내주인 A주로부터 해외 주인 홍콩 H주로의 상호인과관계가 크게 개선되지 못했다. 이는 정보비대칭가설은 설득력이 없음을 시사한다. 종합적으로 요약하면 양 시장 간의 정보 전이에서 지수 간에는 상호 영향을 미치고 개방 후 상호 영향력은 증대 하였다. 그러나 중국시장과 홍콩시장 간에 지수 및 교차상장 된 개별 종목의 가격차이분석에서 본국주도가설이나 정보비대칭가설은 설득력이 없고 시장의 다른 특성에서 찾아야한다고 사료된다.