The demand for knowledge transfer and experience sharing in terms of digital government and smart cities among partner countries has significantly increased. Digital government-related projects have been mainly proposed for the convenience of the system providers for the short-term or one-time instance without considering the mid-term to the long-term direction of digital government in partner countries. Due to this limitation, after the Knowledge Transfer projects are completed, the government partner countries that participated in the projects have examined the Knowledge Transfer projects and need to build improvement plans to promote the related project in efficient, effective, and sustainable ways with mid-term to long-term perspectives. This study focuses on the knowledge transfer projects in the digital government and smart cities that the Korean government and major public institutions carried out and analyses the status of digital knowledge transfer or official development assistance (ODA) projects from 2011 to 2020. In doing so, the study looked into establishing public sector systems such as digital government and smart cities and analysed the current status and research results of domestic and foreign studies related to assessment and diagnosis. Through this, major factors that determine the performance of digital government ODA were identified, and an analysis framework was derived for the analysis of existing cases of digital government knowledge transfer implementation.

본 연구는 강원도 영월군에 위치하는 한반도습지에 분포하는 관속식물상을 파악하여 내륙습지 생태계의 식물 자원 관리보전을 위한 기초자료로 활용하고자 하였다. 조사는 2019년 4월부터 2020년 5월까지 총 9회에 걸쳐 수행하였다. 조사결과 분포가 확인된 관속식물은 93과 309 속 456종 10아종 37변종 5품종 등 총 508분류군이었다. 이 중 멸종위기 야생식물은 II급에 해당하는 2분류군이 확인 되었으며, 희귀식물은 8분류군이었다. 특산식물은 8분류 군이었으며, 식물구계학적 특정식물은 V등급에 3분류군, IV등급에 10분류군, III등급에 15분류군, II등급에 17분류군 그리고 I등급에 26분류군으로 총 71분류군이었다. 귀 화식물은 57분류군이 확인되었으며, 이에 의한 귀화율은 11.2%, 도시화지수는 17.8%로 산출되었고, 생태계교란 야생식물은 5분류군이 분포하는 것으로 조사되었다. 본 연구결과는 람사르습지에 등재되어 있어 보전가치가 매우 높은 한반도 습지의 효율적인 관리를 위한 유용한 기초자료로 활용될 것으로 판단된다.

The incidence of stomach cancer has been found to be gradually decreasing; however, it remains one of the most frequently occurring malignant cancers in Korea. According to statistics of 2017, stomach cancer is the top cancer in men and the fourth most important cancer in women, necessitating methods for its early detection and treatment. Considerable research in the field of bioinformatics has been conducted in cancer studies, and bioinformatics approaches might help develop methods and models for its early prediction. We aimed to develop a classification method based on deep learning and demonstrate its application to gene expression data obtained from patients with stomach cancer. Data of 60,483 genes from 334 patients with stomach cancer in The Cancer Genome Atlas were evaluated by principal component analysis, heatmaps, and the convolutional neural network (CNN) algorithm. We combined the RNA-seq gene expression data with clinical data, searched candidate genes, and analyzed them using the CNN deep learning algorithm. We performed learning using the sample type and vital status of patients with stomach cancer and verified the results. We obtained an accuracy of 95.96% for sample type and 50.51% for vital status. Despite overfitting owing to the limited number of patients, relatively accurate results for sample type were obtained. This approach can be used to predict the prognosis of stomach cancer, which has many types and underlying causes.

내시경 역행 췌담관조영술 중 경췌중격절개술은 선택적 담관 삽관이 어려운 환자에서 성공률을 높일 수 있는 방법으로 알려져 있다. 최근 후향적 연구에서는 경췌중격절개술이 침형절개도를 이용한 조임근절개술보다 효과적이며 안전한 것으로 보고되었다. 그러나 이번 증례에서 담석췌장염 환자에서 경췌중격절개술 후 벽내 십이지장 혈종 및 십이지장 폐쇄가 발생하였고 이로 인해 저혈량쇼크 등 치명적인 합병증이 발생하였다. 따라서 급성 췌장염이 동반된 환자에서 내시 경역행췌담관조영술 중 경췌 중격절개술을 시행한 후에는 벽내 십이지장 혈종과 같은 합병증 발생 여부를 주의 깊게 관찰할 필요가 있다고 생각된다.

This study was carried out to investigate the flora of Mt. Baegam (1,099 m) from March, 2014 to July, 2015. The vascular plants were summarized as 506 taxa, including 90 families, 293 genera, 435 species, 5 subspecies, 58 varieties and 8 forms. Among the investigated 506 taxa, 14 Korean endemic, 16 rare plants and 80 specially designated plants (Ⅴ degree: 3 taxa, Ⅳ degree: 7 taxa, Ⅲ degree: 22 taxa, Ⅱdegree: 21 taxa, Ⅰ degree: 27 taxa) by the Ministry of Environment were also included. The naturalized plants were 36 taxa including 3 ecosystem disturbance wild plant. The urbanization index and percent of naturalized plants species were estimated as 11.2% and 7.1%, respectively. Useful plants of 506 taxa listed consists of 207 taxa (40.9%) of edible plants, 200 taxa (39.5%) of pasture plants, 146 taxa (28.9%) of medicinal plants, 58 taxa (11.5%) of ornamental plants and 15 taxa (3.0%) of timber plants, respectively.

개병풍(Astilboides tabularis (Hemsl.) Engl.)의 보전 및 복원을 위한 기초자료를 확보하기 위해 자생지 환경특성을 조사하였다. 자생지 고도는 해발 432-1,413 m까지 다양하게 위치하 였으며, 경사도는 16-49.5°로 비교적 급한 경사지에 자라는 것 으로 확인되었고, 주로 북사면을 선호하는 것으로 나타났다. 개 병풍 자생지의 식생분석 결과 12개 방형구에서 조사된 관속식 물은 246분류군이었으며, 개병풍이 주로 분포한 초본층의 중요 치는 개병풍이 20.40%로 가장 높았고, 다음으로는 관중(4.56%), 도깨비부채(3.72%), 노루오줌(2.81%), 대사초(2.67%), 단풍취 (2.65%), 벌깨덩굴(2.46%)의 순으로 나타나 이러한 종류들이 개병풍과 유사한 환경을 선호하는 것으로 판단된다. 종다양도는 평균 1.23, 우점도와 균등도는 각각 0.11과 0.86으로 산출되었다. 토양분석 결과 낙엽층의 두께는 2-10 ㎝이었으며, 토심 은 20.8-67.5 ㎝로 나타났다. 토성은 사질토, 미사질토, 양토 등이었으며, pH는 5.63-7.74, 유기물함량은 8.88-42.55%, 그 리고 질소함량은 1.40-6.58 ㎎/g으로 나타났다.

Recently, we reported growth arrest-specific gene 6 (Gas6) as a new maternal effect gene (MEG), that expressed in the oocytes but functioned principally during embryogenesis. Gas6 RNAi-treated oocytes developed to metaphase II (MII) stage but they have affected M-phase promoting factor (MPF) activity and incurred abnormal pronuclear (PN) formation during fertilization. Gas6 is a ligand of TAM family members (Tyro3, Axl and Mertk) of receptor tyrosine kinase (RTK). Purpose of the present study was to evaluate the expression of Tyro3, Axl and Mertk transcripts in oocytes and early embryos. Expression of Gas6 and Mertk mRNA was detectable in oocytes and follicular cells, while Tyro3 and Axl mRNA was expressed only in follicular cells. Expression of Mertk mRNA was relatively constant during oocytes maturation and embryogenesis, but the other receptors, Tyro3 and Axl, were not expressed in oocytes and PN stage of embryos at all. Knockdown of Mertk mRNA and protein by using sequence-specific Mertk double strand RNA (dsRNA) did not affect oocytes maturation. In this case, however, contrary to the ligand Gas6 RNA interference (RNAi), MPF activity had not been changed by Mertk RNAi. Therefore, we concluded that the Gas6-Mertk signaling is not directly related to the oocyte maturation. It is still required to study further regarding the function of Mertk as the receptor of Gas6 during preimplantational early embryogenesis.

Previously, we found that (Zeta-chain-associated protein kinase) expressed in the mouse oocytes and played significant role in completion of meiosis specifically at MI-MII (metaphase I-II) transition. Microinjection of dsRNA into the cytoplasm of germinal vesicle oocyte resulted in MI arrest, and exhibited abnormalities in their spindles and chromosome configurations. The purpose of this study was to determine the mechanisms of action of in oocyte maturation by evaluating downstream signal networking after RNAi (RNA interference). The probe hybridization and data analysis were used by Affymetrix Gene Chip Mouse Genome 430 2.0 array and GenPlex 3.0 (ISTECH, Korea) software, respectively. Total 1,152 genes were up (n=366) and down (n=786) regulated after RNAi. Among those genes changed, we confirmed the expressional changes of the genes involved in the regulation of actin cytoskeleton and MAPK (mitogen-activated protein kinase) signaling pathway, since the phenotypes of RNAi in oocytes were found in the changes in the chromosome separation and spindle structures. We confirmed the changes in gene expression in the actin skeletal system as well as in the MAPK signaling pathway, and concluded that these changes are main cause of the aberrant chromosome arrangement and abnormal spindles after RNAi.

Previously, we have shown that Bcl2l10 as a member of Bcl-2 family, key regulators of the apoptotic process, is dominantly expressed in oocytes of ovary but several member of the Bcl-2 family are not expressed in oocytes. Recent our studies had been processed about roles and regulatory mechanisms of Bcl2l10 in oocytes. Microinjection of Bcl2l10 RNAi into the cytoplasm of germinal vesicle oocytes resulted in metaphase I (MI) arrest and exhibited abnormalities in their spindles and chromosome configurations (Yoon et al., 2009). The present study was conducted to elucidate the downstream genes regulated by Bcl2l10 and signaling networks in Bcl2l10 RNAi microinjected oocytes by using microarray analysis. Surprisingly, we found that a large proportion of genes regulated by Bcl2l10 RNAi were involved in the cell cycle and actin skeletal system regulation as important upstream genes of Bcl2l10. Among the transcripts with highly significant fold changes more than 2-fold, Tpx2 and Cep192 are 16.1- and 8.2-fold down regulated respectively by Bcl2l10 RNAi. Tpx2 and Cep192 are known as cofactors that control Aurora A kinase activity and localization. Therefore, we concluded that Bcl2l10 may have important roles during oocyte meiosis as functional upstream regulator of Tpx2 and Cep192.

This study intended to investigate environmental factors including soil and vegetation in order to understand the environmental and ecological characteristics of 12 different habitats of Iris odaesanensis. These habitats, according to investigations, are mostly located at elevation of 280 m to 1,555 m with angles of inclination ranging from 2 degree to 30 degrees. A total of 273 vascular plants are identified in 23 quadrates of 12 habitats. Dominant species of woody plants in 12 habitats are represented as Quercus mongolica in the tree layer (T1) and the subtree (T2) layer, and Lespedeza maximowiczii, Lindera obtusiloba, Rhododendron schlippenbachii in the shrub (S) layer. The importance value of Iris odaesanensis is 9.65%, as regards the herbaceous layer, and 6 highly ranked species such as Carex siderosticta (3.92%), Meehania urticifolia (2.67%), Spodiopogon cotulifer (2.58%), Aconitum pseudolaeve (2.51%), Carex bostrychostigma (2.28%) and Disporum smilacinum (2.09%) are considered to be an affinity with Iris odaesanensis in their habitats. The degree of their average species diversity is 1.32, and that of dominance and evenness are 0.08 and 0.89, respectively. The type of soil is sandy loam and loam, and the average field capacity of soil is 28.31%. Their average organic matter is 16.71%, soil pH 5.29, and available phosphorus is 9.29%. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of Iris odaesanensis is correlated with pH and dominance, and species richness is positive related with species diversity.

Previously, we obtained the list of genes differentially expressed between GV and MII oocytes. Out of the list, we focused on functional analysis of Zap70 in the present study, because it has been known to be expressed only in immune cells. This is the first report about the expression and its function of Zap70 in the oocytes. Synthetic 475 bp Zap70 dsRNA was microinjected into the GV oocytes, and the oocytes were cultured in vitro. In addition to maturation rates, meiotic spindle and chromosome rearrangements, and changes in expression levels of transcripts of three kinases, Erk1/2, JNK, and p38, were determined. Zap70 is highly expressed in immature GV oocytes, and gradually decreased as oocyte matured. When dsRNA of Zap70 was injected into the GV oocytes, Zap70 mRNA specifically and completely decreased by 2 hr and its protein expression also decreased significantly. Absence of Zap70 resulted in maturation inhibition at meiosis I (57%) with abnormalities in meiotic spindle formation and chromosome rearrangement. Concurrently, mRNA expression of Erk2, JNK, and p38, were affected by Zap70 RNAi. Therefore, we concluded that Zap70 is involved in MI-MII transition by affecting expression of MAP kinases.