본 연구는 농업유전자원센터에 보존되어있는 불가리아 재래종 고추 유전자원 61 점을 대상으로 농업형질을 조사하고, 22개의 분자마커(SSR marker)를 이용하여 불가리아 재래종 고추 유전자원의 유전적 다양성 및 집단분석을 통하여, 자원보존 및 효율적인 작물 육종을 위한 기초정보를 제공하고자 본실험을 수행하였으며 결과는 다음과 같다. 1. 파종 후 발아까지 소요일수는 최소 11일에서 최대 26 일,평균 16.9 일이었고 개화 소요일수는 최소 48일, 최대 65일, 평균 56.9일이었으며 성숙까지의 소요일수는 최소 73일, 최대 98일, 평균 90.7일이었다. 2. 농업형질의 특성을 바탕으로 PCA 분석을 이용하여 불가리아 고추의 다양성을 분석한 결과, 파종 후 개화까지의 소요일수에 따라 조생종, 중생종, 만생종 3개의 그룹으로 나눌 수있었다. 3. 61점의 고추자원에 대하여 22개 SSR 마커에 의해 나타난 대립유전자 (allele)수는 총 82개였다. 마커당 평균 allele수는 3.7 개였고, allele 수의 범위는 2개에서 5개로 확인되었다. 유전적 다양성을 나타내는 PIC 값의 범위는 0.061-0.636이었으며 평균 PIC 값은 0.349로 확인되었다. 4. 분자마커(SSR)를 이용하여 UPGMA, PCoA, STUCTURE 분석을 통한 고추의 다양성 및 집단 구조를 분석한 결과, 3개의 그룹으로 나뉘어졌다. 결론적으로, 농업형질 특성을 바탕으로 한 불가리아 재래종고추의 다양성과 분자학적 특성을 이용한 다양성 결과와는 차이가 있었다.

Predation, development, and ovipostion experiments were conducted to evaluate Amblyseius swirskii (Athias-Henriot) (Acari: Phytoseiidae) as a potential biological control agent for tomato russet mite, Aculops lycopersici (Massee) (Acari: Eriophyidae) which is a periodic pest of greenhouse tomatoes. Results show that A. swirskii attacked all developmental stages of A. lycopersici, and had a type II functional response on the prey densities given. The predation rates of A. swirskii on A. lycopersici in the presense of alternative food sources such as pollen, thrips first instar, or whitefly eggs were recorded as 74%, 56%, and 76%, respectively of the predation rate on A. lycopersici alone. Amblyseius swirskii successfully completed their life-cycle on either A. lycopersici or cattail pollen. At 25oC, 70% RH, development time of female A. swirskii fed on A. lycopersici or on cattail pollen took 5.0 and 6.2 days, respectively. For the first 10 days after moulting to the adult stage, A. swirskii fed on A. lycopersici had higher daily oviposition rate (2.0 eggs per day) than on pollen (1.5 eggs per day). From this laboratory study, it can be concluded that A. swirskii has promising traits as a predator against A. lycopersici and that their populations can be stably maintained using alternative food such as cattail pollen. We suggest that the effectiveness of A. swirskii against A. lycopersici under field conditions deserves to be investigated.

The ferroelectric properties of barium titanate strongly depend on its microstructure, in particular, grain size and distribution. During sintering, usually exhibits abnormal grain growth, which deteriorates considerably the ferroelectric properties. A typical technique to suppress the abnormal grain growth is the addition of dopants. Dopant addition, however, affects the ferroelectric properties and thus limits the application of . Here, we report a simple but novel technique to prevent the abnormal grain growth of and to overcome the limitation of dopant use. The technique consists of stepwise sintering in a reducing atmosphere and in an oxidizing atmosphere. The materials prepared by the present technique exhibit uniform grain size and high dielectric properties. The technique should provide opportunities of having -based materials with superior ferroelectric properties.

Raman spectroscopy has been used to investigate the structure of coal tar pitch heat-treated up to 3000℃ by using 514.5 run Ar ion laser line. Four critical temperature ranges were found on pyrolyzing coal tar pitch, which correspond to four distinct processes from disordered carbons to the well-ordered graphite structure. The range of heat treat temperature (HTT) below 1000℃ corresponds to gas evolution during the pyrolysis of coal tar pitch. Above the HTT are correlated to rearrangements of enlarged molecules, growth of the molecules along the direction of plane, finally stacking in the normal direction of the plane, in the respective HTT ranges of 1000-2000, above 2000 and 2500-3000℃.

Open clusters are useful tools to investigate the structure and evolution of the Galactic disk. We have started a long-term project to obtain UBVI CCD photometry of open clusters which were little studied before, using the Doyak 1.8 m telescope of Bohyunsan Optical Astronomy Observatory in Korea. The primary goals of this project are (1) to make a catalog of UBVI photometry of open clusters, (2) to make an atlas of open clusters, and (3) to survey and monitor variable stars in open clusters. Here we describe this project and report the first results based on preliminary analysis of the data on four open clusters in the survey sample: Be 14, Cr 74, Biu 9, and NGC 2355. Isochrone fitting of the color-magnitude diagrams of the clusters shows that all of them are intermediate age to old (0.3-1.6 Gyrs) open clusters with moderate metallicity.

Tomatoes are considered as one of the main components of daily meals in most households. Thus, it is important to invest in studies enhancing their yield and nutritional value. The study evaluated the effect of wood vinegar (WV) on tomato under greenhouse conditions. Data on fruit number, fruit weight, and plant height were recorded. Total phenolic content (TPC) and antioxidant activity of tomato were determined using the Folin-Ciocalteu reagent and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, respectively. FRR-CF+500x-WV significantly increased the fruit number (86.11%) and fruit weight (81.78%) over the control. Results were comparable to HRR-CF+250x-WV, indicating that conventional fertilizer and WV may have synergistic effect on each other. TPC and scavenging effect on DPPH radical was improved by 250x-WV application. The increase in the phenolic compounds can be attributed to WV as a direct source or as a factor triggering the plants to produce more secondary metabolites with the purpose of increasing natural defenses. The significant effect obtained by applying the full recommended rate of conventional fertilizer on the carotenoid content was due to the availability of the major nutrients needed by the plant. Correlation analysis showed that carotenoids have negative correlation with TPC and DPPH. However, TPC and DPPH showed that these are positively correlated with each other. Tomatoes are excellent source of antioxidants associated with the reduction of some human diseases and improved health. The results provided evidence that WV alone and/or its combination with conventional fertilizers has favorable effects on the quality of tomato.

This study characterized PM and VOC emissions from cow dung combustion in a controlled experiment. Dung from grass-fed cows was dried and combusted using a dual cone calorimeter. Heat fluxes of 10, 25, and 50 kW/m² were applied. The concentrations of PM and VOCs were determined using a dust spectrometer and gas chromatography/mass spectrometry, respectively. PM and VOC emission factors were much higher for the lower heat flux, implying a fire ignition stage. When the heat flux was 50 kW/m², the CO₂ emission factor was highest and the PM and VOC emission factors were lowest. Particle concentrations were highest in the 0.23-0.3-μm size range at heat fluxes of 25 kW/m² and 50 kW/m². Various toxic VOCs including acetone, methyl ethyl ketone, benzene, and toluene were detected at high concentrations. Although PM and VOC emission factors at 50 kW/m² were lower, they were high enough to cause extremely high indoor air pollution. The characteristics of PM and VOC emissions from cow dung combustion indicated potential health effects of indoor air pollution in developing countries.

This study was done to understand the G x E interaction of rice blast reaction for Japonica high quality rice varieties and to observe blast pattern for high quality varieties. Twenty one percent of the total sum of squares (SST) in blast reaction data of high quality Japonica varieties is attributed to genotype (G) by environment (E) interaction variation. This portion of blast response is higher than 8～12% of G x E effect in blast severity data obtained from various ecotypes of rice varieties. Blast response scores obtained from high quality Japonica varieties group were more severely affected by environment condition than mixed groups with Japonica and Indica varieties. Interaction Principal Component Analysis (IPCA) scores obtained from AMMI analysis for the leaf blast response implied variation of G x E interaction. Correlation analysis suggested that IPCA1 was associated with latitude, maximum mean temperature, precipitation and mean cloud amount. IPCA2 was associated with mean relative humidity, and IPCA3 was associated with precipitation and minimum relative humidity. Pattern analysis generated nine genotype clusters according to blast reaction over 11 regions. Collectively, the A, B, C, and D groups were susceptible to rice blast, where as the E, F, G, H, and I groups were relatively resistant to rice blast through multi-location blast nursery test. Relationship between the identified genes of high quality varieties and blast scores at each test site in the level of group could be analyzed based on the results from G x E Interaction analysis.

Mainly due to deficiencies in nuclear reprogramming, gene expression and DNA fragmentation, which result in early and late embryonic losses, the overall success rate achieved by cloning techniques to date is low. This present study compared the incidences of DNA fragmentation during development of IVF, parthenotes (PT), nuclear transfer (NT) and transgenic (TG) embryos. Terminal deoxynucleotidyl transferase (TdT) nick-end labelling (TUNEL) with propidium iodide counter staining was used for determination of DNA fragmentation and total number, respectively. TG and NT donor cells were fetal fibroblasts with or without transfection with EGFP, and cultured in DMEM+15% FCS until confluent, for 5 days. At 19 h post-maturation (hpm), enucleated oocytes were reconstructed with donor cells and activated at 24 hpm with the combinations of ionomycin (5 M, 5 min) and cyclo-heximide (10 g/ml, 5 h) after electric fusion by a single DC pulse (1.6 KV/cm, 60 sec). Parthenotes were produced by the same activation protocol at 24 hpm. (중략)