Background: Largemouth bass (Micropterus salmoides) is introduced species that has caused aquatic ecology activity both in vitro and in vivo were investigated for the possibility of application of the bass extract as an alternative feed ingredient. Methods: The bass oil was extracted using a 1-L supercritical extractor, while the protein was extracted from 250 g of bass dry matter, which was dissolved in 1 mL of H2O at 50℃. Both oil and protein extracts were evaluated antioxidant activities and the level of DPPH radical scavenging assay and nitric oxide (NO) production assay with lipopolysaccharide response. Oral administration of 6.6 μL/g bass protein and 5.38 μL/g bass oil conducted for investigating serological and physiological effect. Results: DPPH radical scavenging showed similar radical scavenging ability of 50 μM of ascorbic acid at 200 μg of protein and 10% of oil treatment. NO concentration was diminished by the treatment of bass oil. Oral administration of both bass oil and proteins to mice showed that the body weight increase rate of the bass oil treated group was significantly reduced by 1.55 g compared to the other groups. The number of white blood cells (WBC) was increased by 4.52 k/μL in the bass protein-treated group and 4.44 k/μL in the bass oil-treated group compared to the control group. However, the serum IgG level did not show a significant difference between the bass extract-treated groups and the control group. Conclusions: These studies demonstrate that both bass oil and proteins extracted from the bass not only provide excellent effects of antioxidant and immune activity but can also be used as functional food supplements.

식물플랑크톤에 의한 요소의 분해속도와 요소형태질소의 흡수속도를 한강수계에 위치한 6개의 인공호의 표수층(0~3m)에서 측정하였다. 명조건하에서 측정한 분해속도와 흡수속도는 각각 2~70 μg ureaC m-3 h-1과 7~170 μg ureaN m-3 h-1였고, 암조건하에서는 각각 1~16 μg ureaC m-3 h-1과 3~120 μg ureaN m-3 h-1였다. 이들 속도는 클로로필 a 현존량 및 광합성과 상관관계가 있었다. 1일 당 분해량과 흡수속도는 각각 0.1~1.0 mgC m-3 day-1과 0.1~3.8 mgN m-3 day-1이였고, 이들 속도로부터 표수층에서 요소의 체류시간은 1~100일로 추정되었다. 요소의 분해속도와 요소형태질소의 흡수속도가 보여준 양의 상관관계와 양자의 비는 요소가 분해된 양만큼의 요소형태질소가 식물플랑크톤의 질소원으로 이용되었다는 것을 나타낸다.

Harmonized actions of ovarian estrogen (E2) and progesterone (P4) regulate cell proliferation and differentiation in the uterus with a spatiotemporal manner. Imbalance between the actions and levels of two major regulators often lead to infertility and gynecological diseases, such as endometriosis and endometrial cancer. While numerous works have shown that reduced expression and/or deletion of uterine factors associated with P4 signaling could disturb uterine physiology, local factor(s) to mediate E2 actions has not been extensively studied yet. Here we demonstrate that early growth response 1 (Egr1), a transcription factor which is rapidly induced in the uterus by E2, is required to maintain coordinated actions of E2 and P4 for uterine receptivity for embryo implantation. Given exogenous gonadotrophins to overcome LHβ deficiency in the pituitary of Egr1(－/－) mice, ovulation, fertilization and embryo development normally occurred in these mice. However, they showed complete failure of embryo implantation with reduced uterine responses to artificial decidualization stimuli. While serum levels of E2 and P4 in Egr1(－/－) mice were comparable, genes regulated by E2 and/or P4 in uterine epithelial cells (ECs) were aberrantly expressed on day 4 of pregnancy. Impaired P4 signaling along with absence of PR in ECs caused hypersensitive E2 responses shown as enhanced expression of E2-responsive genes such Muc1 and Ltf as well as reduced levels of P4-dependent genes, such as Ihh and Areg, in ECs of Egr1(－/－) mice. This is consistent with persistent proliferation in ECs and severely impaired proliferation in stromal cells (SCs) in Egr1(－/－) mice treated with E2+P4. Furthermore, primary co-culture of Egr1(－/－) ECs with Egr1(+/+) SCs and vice versa supported a notion that Egr1 itself is required for proper responses to two major regulators, E2 and P4, in both uterine cell compartments. Collectively, our results show that E2-induced Egr1 participates in P4-dependent modulation on E2 activities in the uterus by regulating a spectrum of genes essential for uterine receptivity and embryo implantation.

DGCR8 is a RNA-binding protein working with DROSHA to produce pre-microRNA in the nucleus, while DICER does not only mature microRNA but also endogenous siRNAs in the cytoplasm. Here, we have produced Dgcr8 conditional knock-out mice using progesterone receptor (PR)-Cre (Dgcr8flox/flox; PRcre/+ mice, Dgcr8d/d) and demonstrated that canonical microRNAs dependent of DROSHA-DGCR8 complex are required for uterine development as well as female fertility in mice. Adult Dgcr8d/d females did not undergo regular reproductive cycle and produce any pups when housed with fertile males, whereas administration of exogenous gonadotropins induced normal ovulation with corpus luteal formation in these mice. Ovulated oocytes from Dgcr8d/d mice had comparable fertilization potentials and were normally developed to the blastocyst after fertilization as compared to those in control Dgcr8f/f mice. Interestingly, PR-Cre-dependent Dgcr8 deletion showed aberrant infiltration of acute inflammatory immune cells to female reproductive organs only when Dgcr8d/d mice were mated with male mice. With respect to uterine development, gross morphology, histology, and weight of Dgcr8d/d uterus were similar to those of control at 3-week-old age. However, multiple uterine abnormalities were noticeable at 4-week-old age when PR expression is significantly increased, and these deformities became severe onwards. Gland formation and myometrial layers were significantly reduced, and stromal cell compartment did not expand and became atrophic during uterine development in these mice. These results were consistent with aberrantly reduced cell proliferation in stromal cell compartments of Dgcr8d/d mice. Collectively, our results suggest that DGCR8 dependent-canonical microRNAs are essential for development and physiology of the uterus with respect to morphogenesis, proper immune modulation, reproductive cycle, and steroid hormone responsiveness in mice.

This study was performed in 30 selected apartments in Seoul, Asan and Daegu area which were constructed within 4 years and over 4 years, to measure the concentration of VOCs(benzene, toluene, xylene) from July, 2004 to September. Mean ratios of indoor to outdoor VOCs concentrations in the construction under 4 years were higher in 1 than average, I/O ratio of over 4 years were lower in 1. This was considered that the VOCs density influences indoor pollutant. For the indoor air quality estimation, the deposition constant and the source strength factor of toluene were 1.49±2.05 hr-1 and 36.95±52.26 ppb/h, respectively.

A new Cymbidium hybrid “Bright Evening” was released by National Horticultural Research Institute (NHRI) in 2003. A cross was made in 1995 between Cymbidium “Tropical Yellow”, a brownish yellow colored petal and medium plant sized variety, and “Lucky Rainbow Rapin Hot”, a light purple-pink petal with red lip and medium sized variety. A cross was made in 1995 between Cymbidium “Tropical Yellow”, a brownish yellow colored petal and medium plant sized variety, and “Lucky Rainbow Lapin Hot”, a light purple-pink petal with red lip and medium sized variety. The eighty seven progenies were obtained after planting and acclimatization in green house. In 1999, a line (9529125) was selected and multiplicated for its flower color, leaf shape, flower stalk, and vigorous growing habit. After evaluation trial for two growing seasons, the selected line was named as “Bright Evening”. The “Bright Evening” has yellow petals and is a medium sized (average leaf length is about 62 cm and width of flower is about 6 cm) Cymbidium variety with vigorous growth and erect flower stalks. Blooming is started from November to February in optimal culture condition. High temperature (more than 30℃) and low light intensity should be avoided for the appropriate growth. In addition, flower stalks develop easily even from underdeveloped pseudobulbs.

The work of hairdressers includes washing, coloring, bleaching, permanent waving, conditioning, and cutting hair. Hairdressers are subjected to a number of physical and toxicological hazards. The toxicological hazards are those resulting from exposure to a wide range of chemicals that are usually classified active processes. In this study, twenty beauty shops were selected to assess the exposure to indoor air pollutants such as VOCs and particulate matter (PM10) during one month from September 1 to September 30, 2003. Indoor air quality of beauty shops might be worse by vehicle emissions because the beauty shops were generally located near roadways. Personal exposures to VOCs and PM10 were related to indoor concentrations of beauty shops. According to the questionnaire, hairdressers complained of sore throat, eye irritation, and nervousness as physical symptoms. The measured mean concentrations of respiratory particulates were 30.5ng/㎥ in indoor, 30.5ng/㎥in outdoor and 44.0ng/㎥ on personal levels. The personal concentration was found higher than indoor and outdoor concentrations. The heavy metals mean concentrations were shown as indoor (Na>Zn>Cr), outdoor (Cr>Zn>Pb), and personal (Na>Cr>Zn) levels. Conclusively, customers as well as workers in the beauty shops might be highly exposed to air pollutants from indoor and outdoor sources. Therefore, proper management should be taken to improve the indoor air quality in beauty shops.