Twenty two common millet (Panicum miliaceum L.) varieties collected from Korea, China and Russia were investigated for their phylogenetic relationship using 5S ribosomal DNA sequences with a hope to provide the basic information on their exact origin. Sequences of 5S rDNA were isolated by PCR. The primers, 5s-rRNA1 and 5s-rRNA2, were designed to isolate the complete NTS. Genomic DNA amplification produced two fragments with different length, 900 bp and 400 bp fragments, confirming the presence of two types of 5S rDNA repeats that differed from each other in the length of the NTS region. Amplified DNAs of 400 bp fragment were subcloned and used for further investigation. The obtained NTS sequences ranged from 200 to 300 bp and homology of sequences among plant materials was much higher than long repeat. CLUSTALW multiple aligment of 5S rDNA sequences from 22 different common millets revealed the clear difference by their origin. And critically different areas with insert or deletion were also confirmed. Those sequence difference seems to be used for discrimination of cultivars from different origin and use as molecular markers for origin identification. In phylogenic tree construction, the clear classification was shown where the genotypes from China and Russia is positioned together and stay away from domestic genotypes.

Korean soybean variety Kwangan was transformed with coat protein (CP), helper component-proteinase (HC-Pro), and ABRE binding factor 3 (ABF3) genes using highly efficient soybean transformation system. Among these genes, CP and HC-Pro were transformed using RNAi technology. Transgenic plants with CP were confirmed for gene introduction and their expression using PCR, real-time PCR, RT-PCR, Southern blot, and Northern blot. To investigate the response of viral infection with CP, T1 plants were inoculated with SMV-infected leaves and confirmed the existence of mosaic symptom in both leaves and seeds. Two transgenic lines with CP were highly resistant to SMV with clear leaves and seeds while SMV-susceptible lines showed mosaic symptom with seed mottling. The transcript levels of T1 plants with CP were also determined by northern blot, suggesting that SMV-resistant T1 plants did not show viral RNA expression whereas SMV-susceptible T1 plants showed viral RNA expression. Currently, the response of viral infection with HC-Pro is investigating to produce SMV-resistant soybean transgenic plants, and the physiological experiment with ABF3 is also carrying out to produce drought-tolerant soybean transgenic plants.

The aim of this study was to evaluate 30 phenolic compounds in adzuki bean germplasm. Adzuki 21653 had the highest content of total phenolics compounds (6597~;~mug~;g-1 ) while 104372 had the lowest concentration. The average total phenolic content of Japanese (2432~;~mug~;g-1 ) adzuki beans was higher than that of Korean (2256~;~mug~;g-1 ) adzuki beans. The average total phenolic contents were 2507~;~mug~;g-1 in small sized adzuki beans from Japan and 2459~;~mug~;g-1 in those from Korea. In large sized adzuki beans, the average total phenolic contents were 1315~;~mug~;g-1 in Japanese seeds and 1232~;~mug~;g-1 in Korean seeds. The average total phenolic contents in medium seeds were 2369~;~mug~;g-1 in Japanese adzuki beans and 1397~;~mug~;g-1 in Korean ones. In small seeds, the total phenolic contents of adzuki beans varied from 524~;~mug~;g-1 to 6597~;~mug~;g-1 in Japanese ones and from 375~;~mug~;g-1 to 6569~;~mug~;g-1 in Korean ones. Japanese and Korean adzuki beans were divided into landraces and wild adzuki beans. In this study, the wild adzuki beans showed higher contents of total phenolics than the native varieties. Specifically, the wild adzuki beans from Korea had the highest concentration of phenolics (3403~;~mug~;g-1 ). All adzuki bean germplasms were measured for their color and were classified into four groups accordingly: A; L < 30, +a, +b; B; L < 30, +a, -b, C; L > 50, +a, +b, D; L > 50, +a, -b. Especially, group B had the highest concentration of total phenolic compounds (2827~;~mug~;g-1 ), whereas group C had the lowest concentration (1882~;~mug~;g-1 ).

본 연구는 나리의 종간잡종 육성체계를 확립하는데 필요한 기초자료를 얻기 위하여 OA hybrid 중 자발적으로 생식세포 염색체가 배수화된 계통을 이용하여 후대 획득과 후대검정에 필요한 실험을 수행하여 얻은 결과를 요약하면 다음과 같다. 2n 화분을 생산하는 OA hybrid의 경우 화분의 채취시기에 따른 생존율과 발아율은 10월에 비해 6월이, 생존율은 n성 화분이, 발아율은 2n성 화분이 높았다. 2n 화분을 생산하는 2배체 OA hybrid와 2배체