The purpose of this study was to investigate antioxidant characteristics and anti-adipogenic effects of colored rice and brown rice extracts in 3T3-L1 adipocyte depending on cultivar (Josaengheugchal, Heugjinmi, Hongjinju, Geongganghongmi, Seolgaeng, Milyang 320, Sindongjin, Baegjinju). Colored rice and brown rice was extracted with 100% ethanol, followed by the analysis of polyphenol, flavonoid, anthocyanin, antioxidant, and anti-adipogenic activity. Total polyphenol and flavonoid content ranged from 6.86~314.08 mg GAE/g and 1.47~56.88 mg CE/g the highest total polyphenol and flavonoid content was observed in Heugjinmi cultivar. Anthocyanin composition was analyzed by HPLC, cyanidin-3-gluoside and peonidin-3-glucoside was found in black rice including Josaengheugchal and Heugjinmi. Also, the ABTS and DPPH radical scavenging activity of colored rice cultivars was higher than that of brown rice cultivars, the highest ABTS radical scavenging activity also was observed in Heugjinmi (128.20 mg TE/g). The anti-adipogenic effects of colored rice and brown rice extracts on differentiation of 3T3-L1 preadipocytes evaluated that extracts of Heugjinmi cultivar significantly reduced intracellular lipid accumulation. These results provide valuable information for the use of Korean colored rice cultivar as a functional food materials relative to anti-obesity.

“aegjoong” a white winter wheat (Triticum aestivum L.) cultivar was developed by the National Institute of Crop Science, RDA. It was derived from the cross “eumkang”“lgeuru”during 1996. “aegjoong”was evaluated as “ksan307”in Advanced Yield Trial Test in 2004. It was tested in the regional yield trial test between 2005 and 2007. “aegjoong”is an awned, semi-dwarf and soft white winter wheat, similar to “eumkang”(check cultivar). The heading and maturing date of “aegjoong” were similar to “eumkang” Culm and spike length of “aegjoong”were 77 cm and 7.5 cm, similar to “eumkang” “aegjoong”had lower test weight (802 g) and lower 1,000-grain weight (39.8 g) than “eumkang”(811 g and 44.0 g, respectively). It had resistance to winter hardiness, wet-soil tolerance and lodging tolerance. “aegjoong”showed moderate to pre-harvest sprouting (23.9%) although “eumkang”is susceptible to pre-harvest sprouting (38.9%). “aegjoong”had similar flour yield (72.4%) and ash content (0.41%) to “eumkang”(72.0% and 0.41%, respectively) and similar flour color to “eumkang” It showed lower protein content (8.8%) and SDS-sedimentation volume (35.3 ml) and shorter mixograph mixing time (3.8 min) than “eumkang”(11.0%, 59.7 ml and 4.5 min, respectively). Amylose content and pasting properties of “aegjoong”were similar to “eumkang” “aegjoong”had softer and more elastic texture of cooked noodles than “eumkang” Average yield of “aegjoong”in the regional adaptation yield trial was 5.88 MT ha-1 in upland and 5.35 MT ha-1 in paddy field, which was 13% and 17% higher than those of “eumkang”(5.21 MT ha-1 and 4.58 MT ha-1, respectively). “aegjoong” would be suitable for the area above the daily minimum temperature of -10℃ in January in Korean peninsula.

The effects of sonication and vacuum infiltration on transformation efficiency was investigated by using immature embryos of Korean wheat as explants. Two Agrobacterium tumefaciens strains, KYRT1 and EHA105, carrying pCAMBIA 1305.1 were used. Transformation efficiency was demonstrated by the detection of β-glucu-ronidase (GUS) activity. GUS expression showed clear difference among Korean wheat cultivars. Geurumil showed higher GUS expression efficiency 79.1~% compared with other cultivars. The effects of the duration of vacuum infiltration and sonication treatment showed a tendency high GUS expression efficiency by their combination. In comparison with other Agrobacterium strains, KYRT1 showed high efficiency in most Korean cultivars.