Background : This study was conducted to investigate the antioxidant activities and anticancer effect in 80% ethanol extract from foxtail millet (Setaria italica) and proso millet (Panicum miliaceum) and sorghum (Sorghum bicolor) varieties. Methods and Results : These extracts were evaluated for contents of total polyphenol and antioxidant activities using DPPH and ABTS radical scavenging activities. In concentration of 1000 ㎍/㎖, total polyphenol contents of foxtail millet, proso millet and sorghum were 7.68 - 12.74 ㎍․GAE/㎖, 13.01 - 14.79 ㎍․GAE/㎖ and 48.24 - 62.33 ㎍․GAE/㎖, respectively. Sorghum extracts showed strong radical scavenging activity comparable to well-known antioxidant, trolox. Anticancer effect was evaluated for MTT assay using AGS human gastric cancer cells. All extracts decreased growth of AGS cancer cells. Foxtail millet extracts were decreased about 40% of cell viability in all concentrations. Sorghum extracts were decreased cell viability, concentration dependent manner. Especially, Donganme extract (one of the sorghum variety) was the highest anti cancer effect under AGS cancer cells. Conclusion : Based on these results, millet and sorghum has potential health promoting bioactive compounds as functional food.