기후변화는 연안지역에 심각한 영향을 미치고 있으며 그 영향이 점점 증가할 것이라고 예상되는 바, 최근 기후변화 적응 및 리스크 평가에 있어 많은 연구들이 취약성과 함께 회복탄력성 개념을 이용하고 있다. 본 연구의 목적은 기후변화 적응을 위한 연안재해 회복탄력성 측정 모형을 개발하는 것이다. 측정 모형 개발에 앞서 연안재해 회복탄력성에 대한 광범위한 문헌검토를 통해 취약성과 회복 탄력성에 대한 조작적 정의와 함께 여러 피드백 메커니즘이 포함된 개념적 프레임워크를 작성하였다. 연안재해 회복탄력성 측정 모형은 네 가지 측정값(MRV, LRV, RTSPV, ND)과 연안재해 회복탄력성 복합 지수(CRI)를 포함하고 있으며, 개발된 지수는 국내 연안침식 사례에 적용되었다. 또한 지수 등급에 따른 지역적 분석이 수행되었다. 연구 결과, 네 가지 회복탄력성 측정값을 통해 각 지점이 가지는 연안침식 회복탄력성의 다양한 특성을 파악할 수 있음을 확인하였다. 연안 회복탄력성 복합 지수의 매핑 결과 서해안 및 남해안 지역에 비해 동해 안 지역들은 연안침식 회복탄력성이 상대적으로 떨어지는 것으로 나타났다. 본 연구의 회복탄력성 측정 모형은 적응 이후의 이행전략에 대한 논의를 제공하는 도구로 활용될 수 있으며, 서로 다른 취약 지역 그룹 간 정책지원에 대한 우선순위를 결정하는 데 이용 가능하다.
Chrysanthemum boreal, C. indicum, and C. indicum var. albescens are well-known wild Chrysanthemum species used for traditional medicine in Korea. In this study, volatile compounds from three wild Chrysanthemums were identified according to four different flowering stages and analyzed using HS-SPME-GC-MS to determine the temporal variation of the volatiles. As a result, 132, 151, and 142 peaks were identified from C. boreale, C. indicum, and C. indicum var. albescens, respectively. Furthermore, 70 out of 132 peaks were identified in C. boreale with a matching ratio of >90% from library search. In addition, 85/151 and 76/142 peaks were identified from C. indicum and C. indicum var. albescens. Forty-nine volatile compounds were found commonly in all three wild Chrysanthemums through all four different flowering stages. However, six, seven, and five unique compounds were detected only in C. boreale, C. indicum, and C. indicum var. albescens, respectively. One hundred volatile compounds were selected for multivariate analysis considering volatile compounds overlapped with each other. The one-way ANOVA (p < 0.05) detected significant differences from 77 out of 100 volatile compounds. In addition, PLS-DA showed the different profiles of volatile compounds according to four different flowering stages in each wild Chrysanthemum. PC1 of each Chrysanthemum accounted for 45.8 56.9, and 11.9% in C. boreale, C. indicum, and C. indicum var. albescens, respectively. PC1 of C. boreale and C. indicum clearly separated the BF stage and the other three stages. Conversely, PC1 of C . indicum var. albescens showed a difference in the composition of volatile compounds between the BF/BO and HO/FO stages. In addition, the different profiles of volatile compounds could be visualized using a heatmap from three wild Chrysanthemums according to four different flowering stages. This study will help improve particular volatile compounds in three wild Chrysanthemums both in quality and quantity.
Volatile organic compounds (VOCs) in plants are various organic compounds with small molecular weight and high vapor pressure. The metabolomics approach was recently introduced to analyze VOCs involved in biological processes, such as abiotic and biotic stresses, spatial and temporal distribution, and genotypic differences. In addition, this approach is widely used in combination with identification of VOCs analysis and statistical analysis using multivariate analysis, such as principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), hierarchical cluster analysis (HCA), etc. First, in this review, the current condition of the metabolomics approach to analyze VOCs synthesized in plants using head space-solid phase microextraction coupled with gas chromatography-mass spectrometry (HS-SPME-GC-MS) is discussed. In addition, metabolomics approach, such as extraction and analysis of VOCs using HS-SPME-GC-MS, conversion, and processing of mass spectral (MS) data, a database for VOCs identification, useful statistical methods, and statistical tools and applications, are explained. Finally, multi-omics in combination with other omics techniques, such as genomics, transcriptomics, etc. are suggested as prospects of a metabolomics approach for VOC analysis in floricultural plants using HS-SPMEGC- MS. Therefore, the metabolomics approach of HS-SPMEGC- MS will facilitate our understanding of VOCs synthesized in plants. Furthermore, the multi-omics approach will help understand gene functions involved in the biosynthesis of VOCs and help develop new development cultivars with nicer floral scents by contributing to the development of the floricultural industry.
The change in volatile compound composition of three wild chrysanthemum species (Chrysanthemum boreale, C. indicum, and C. indicum var. albescens) was identified and analyzed according to four flowering stages using HS-SPME-GC-MS (headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry). The top five compounds of each flowering stage were selected because those main compounds accounted for 43.25%, 44.14%, and 54.20% of the total relative content of volatile compounds from C. boreale, C. indicum, and C. indicum var. albescens, respectively. Nine compounds (1S-α-pinene, α-thujone, chrysanthenone, umbellulone, thymol, caryophyllene, germacrene D, α-zingiberene, and α-patchoulene) in C. boreale were ranked in the top five compounds through the whole flowering stages. In C. indicum, camphene, eucalyptol, camphor, umbellulone, bornyl acetate, caryophyllene, β-farnesene, germacrene D, and α-zingiberene were ranked in the top five compounds. However, only five compounds (camphor, bornyl acetate, β-farnesene, germacrene D, and α-zingiberene) were ranked in C. indicum var. albescens showing a more stable composition rather than C. boreale and C. indicum. Flowerheads of three wild Chrysanthemums showed a different profile of volatile compounds according to different flowering stages, varying compositions, and relative content in the top five volatile compounds. This study illustrates how main volatile compounds in wild chrysanthemums change dynamically during the flowering regarding compositions and their relative contents, suggesting that it should provide a useful index for harvesting or blending certain target compounds from wild chrysanthemums.
Ornamental chrysanthemum (Dendranthema × grandiflorum Ramat.) belongs to genus Chrysanthemum in the Asteraceae (Compositae) family and is a top leading ornamental plant worldwide. During the last two decades, advances in biotechnology (genetic engineering) and nucleotide sequencing techniques have enabled a deep understanding of biological processes of many ornamental plants at a molecular level. The blue-colored chrysanthemum has been generated and commercialized, and various DNA markers were developed using advanced techniques. Ornamental chrysanthemums are hexaploidy (2n = 2x = 54) and self-incompatible, making it challenging to generate a pure line as breeding resources and a model platform for chrysanthemum research. However, recently, self-compatible pure lines of chrysanthemum (C. seticuspe) were developed, and whole-genome sequencing datasets have been open to the public. Other datasets of whole-genome sequences and transcriptome have been elucidated from wild chrysanthemums, C. nankingense and C. morifolium. In this review, recent progress in the study of ornamental chrysanthemums and the potentiality and importance of wild chrysanthemums as bioresources for ornamental chrysanthemum studies and breeding resources is discussed briefly. Many important resources for studying chrysanthemums are now available and easily accessible. Comprehensive studies with important resources will shed light on chrysanthemum research and breeding of ornamental chrysanthemums at a molecular level. Additionally, this review provides comprehensive information and should pave the way for successful research on ornamental chrysanthemums.
저면관수 방식인 C-형강 매트재배 시스템을 이용한 소형 분화 국화 재배 시 daminozide 농도에 따른 관 주처리, 저면공급 및 정식시기에 따른 생장억제 효과의 지속력 및 저면공급의 처리효과를 구명하고자 본 실험 을 수행하였다. 1차 정식처리구에서 4,000 mg·L-1 이상 의 관주 처리구에서 초장신장 억제효과가 있는 것으로 나타났다. 그러나 2차 정식처리구의 경우 5,000mg·L-1 에서 억제효과가 인정되었다. 분산분석 결과 정식시기도 초장 조절에 영향을 미치는 것을 알 수 있었다. 양액과 저면 공급한 처리구에서 daminozide가 화뢰의 형성, 생 체중 및 건물중에 영향을 미치는 것으로 나타났다. 본 실험을 통하여 정식시기를 조절하여도 초장을 조절 할 수 있음을 알 수 있었으며, 따라서, 초장신장 억제를 위한 생장조절제 처리 시 정식시기와 공급방법의 다양 화를 통해 분화 국화의 효율적인 초장 조절이 가능 할 것이다.
Muscle strength and endurance activities of Korean ginseng (Panax ginseng C. A. Meyer; KG) were compared with those of wild simulated cultivation ginseng (WCG) in mice. Fifty male ICR mice were divided into five groups: A (vehicle); B (WCG 100 mg/kg); C (WCG 500 mg/kg); D (KG 100 mg/kg); E (KG 500 mg/kg). Subsequently, the mice were subjected to the forced swimming test (FST) and treadmill test at the 4th and 7th weeks. The glycogen content in the muscle and blood analysis (levels of glucose, triglyceride (TG), IGF-1) were also performed immediately after the last FST and treadmill test at the 7th week. Immobility times in FST were shorter in WCG- than KG-treated groups, and the results of the treadmill tests were also significant except for KG-treated at 100 mg/kg. The glycogen content was increased in both groups with a peak at 500 mg/kg of WCG groups. Serum concentrations of TG and glucose were decreased by administration of KG and WCG and all treated groups showed increase in the level of IGF-1 in serum. These results suggest that KG and WCG supplementations are effective in escalating the muscle strength and endurance.
본 연구는 실내식물의 시각적 이미지가 주는 심리적 효과와 식물에 대한 관심도와의 관계, 식불에 대한 관심도와 중요도가 충성도에 미치는 영향을 분석하기 위해 실시히였다. 9종의 실내 식물에 대한 14쌍의 이미지 감성형용사 어휘는 평가적인, 활동적인 그리고 정서적인 요인의 세 가지 주요인이 추출되었다. 추출된 세 가지 이미지 감성요인 중 활동적인 요인보디는 평가적인 요인이, 평가적인 요인보다는 정서적인 요인이 실내 식물의 관심도에 미치는 영향이 상대적으로 더 높은 것으로 나타났다(p<.05). 또한 대상자들의 실내식물을 키우는 것에 대한 관심도는 충성도에 매우 긍정적인 영향을 주는 것으로 나타났으나(p<.05) , 연간 구입 개수,구입 비용,실내 식물을 키우는 형태와 같은 행동적 요소는 경우에 따라 유의한 차이가 없는 것으로 나타났다. 실내 식물에 대한 중요성의 인식에 있어서 실내식물에 대한 22항목의 중요도는 6가지 주요인이 추출되었다. 추출된 요인 종 삶의 질적 향상을 위한 "정서이완성”,“심미성”의 개념에 해당하는 항목들의 경우 다른 요인에 비해 충성도에 더 긍정적인 영향을 주는 것으로 나타났다(p<.05). 그러나 5가지 충성도의 항목 중 “실내 식물 구입에 더 많은 비용을 투자할 용의가 있다"는 항목은 다른 항목에 비해 충성도가 상대적으로 낮은 것으로 나타났다. 본 연구를 통해 각 식물의 시각적 이미지가 주는 심리적 효과, 실내식물에 대한 관심도,그리고 중요성 인식은 거주자의 정서적 건강 증진과 실내 식물의 소비를 높이는 데 긍정적인 영향을 줄 수 있는 실내식물에 대한 충성도를 향상시킬 수 있을 것으로 판단된다.
This study was carried out to investigate the difference of growth characteristics, yield and extract content between upland and paddy ginseng cultured with 4year-old ginseng in 2003. Although upland ginseng showed larger variation in yield than that of paddy ginseng, the average of it was greater than that of paddy ginseng because it showed better growth of aerial part and higher survival rate than that of paddy ginseng. Moisture content of fresh root was 71.8~%~;(68.5~~73.1~%),~;and~;72.7~%~;(70.2 ~~74.9~%) on average in upland and paddy ginseng, respectively. Paddy ginseng showed higher hardness in taproot, and higher rate of rusty colored root than that of upland ginseng. The ratio of taproot dry weight in upland ginseng was smaller than that of paddy ginseng, while that of lateral root was larger in upland ginseng. Ratio of marketable root (>60g) to total harvested roots was 13.7~%~;(0.82~~8.0~%)~;and~;7.7~%~;(1.6~~12.6~%) in upland and paddy ginseng, respectively. Extract content did not show distinct difference between upland and paddy ginseng, but it showed large variation from 16.1~;to~;25.1~% in taproot, and from 24.2~;to~;32.5~% in lateral root depanding on the ginseng field examined.