Background : Haskap berries commonly refer to fruits of Lonicera caerulea L., recognized by the Japanese aborigines as the “The elixir of life.”. Due to their recent arrival on the North American market, haskap berries have not yet been positioned among other berries and compared in terms of their phytochemical content. And haskap berries have higher ascorbic acid and anthocyanin content than other berries known for their health-promoting benefits, such as blueberries. However, no study has reported on the antioxidant and anti-cancer activity of Lonicera caerulea stem. The purpose of this study is to present the current research on the chemical content, antioxidant and anti-cancer activities of Lonicera caerulea stem. Methods and Results : The stem of Lonicera caerulea L. ware dried in the shade at room temperature and extracted with 100% methanol. The extract was suspended in deionized water and partitioned sequentially with n-hexane, chloroform, ethyl-acetate and butanol (water saturated BuOH) fractions. Antioxidant activities were measured by determination of antioxidants, DPPH (2,2-diphenyl-1-picrylhydrazyl). Cell viability was determined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. All cell lines were purchased from the Korean Cell Line Bank (Seoul, Korea). All results were performed with three replications were processed statistically. By DPPH assay, the Lonicera caerulea L. the highest activity was obtained from the ethyl-acetate fraction (IC50=15.46 ㎍/㎖). By MTT assay, the chloroform fraction showed a significant growth inhibiting effect on MCF-7 (Human breast cancer, IC50=225.91 ㎍/㎖), COLO 205 (Human colon cancer, IC50=179.55 ㎍/㎖), but on AGS (Human stomach cancer) and other fractions it did not show effect. Conclusion : We demonstrated that Lonicera caerulea L. stem extract and fractions has antioxidant and antiproliferation activity in vitro. Further studies should identify the active constituents in Lonicera caerulea L stem to evaluate the potential in vitro antioxidant and antiproliferation activities of the extract.

본 연구는 창작무용학습이 유아의 지적 발달에 미치는 영향을 검증하기 위하여 수행되었다. 피험자는 D지역의 유치원 두 곳의 유아 만 4,5,6세로 총 69명이었고 실험집단과 통제집단으로 임의 배정하였다. 실험집단의 무용학습은 주 3회 40분씩 12주간 실시하였으며 실험집단과 통제집단의 지능발달 차이를 알아보기 위하여 학습전과 후 지능검사를 하였다. 검사지는 김재은의 유아지능검사 B형으로 실시하였다. 통계분석은 지능발달의 차이를 규명하기위하여 각 지능점수의 평균과 표준편차를 제시하였고 집단간, 연령별, 성별 사전․사후지능점수의 차이를 알아보기 위하여 독립과 반복 t-test를 각각 실시하였다. 모든 통계처리의 유의수준은 .05로 하였다. 결과는 첫째, 창작무용을 학습한 집단이 통제집단보다 지능발달에 유의한 차이가 나타났다. 둘째, 두 집단간의 연령별(4,5,6세) 지능발달차이는 4세, 5세, 6세 모두에서 차이가 있었다. 셋째, 두 집단간에 성별 지능발달은 남여 유아 모두에 차이가 있었다.