In this study, we examined the antagonistic effects of sprout-borne lactic acid bacteria (LAB) on Salmonella enterica serovar Enteritidis. This antagonism is promoted as a means of controlling contamination during sprout production and provides additional LAB for consumers. We isolated a total of 24 LAB isolates in nine species and five genera from seven popular vegetable sprouts: alfalfa (Medicago sativa), clover (Trifolium pratense), broccoli (Brassica oleracea ssp. italica), vitamin (B. rapa ssp. narinosa), red radish (Raphanus sativus), red kohlrabi (B. oleracea var. gongylodes), and Kimchi cabbage (B. campestris var. pekinensis). Based on 16S rRNA gene sequences, the LAB species were identified as Enterococcus casseliflavus, E. faecium, E. gallinarum, E. mundtii, Lactococcus taiwanensis, Leuconostoc mesenteroides, Pediococcus pentosaceus, and Weissella cibaria, and W. confusa. A total of 16 LAB isolates in seven species including E. faecium, E. gallinarum, E. mundtii, L. taiwanensis, L. mesenteroides, P. pentosaceus, and W. cibaria showed antagonistic activity toward S. enterica. The growth inhibition of sprout LAB on S. enterica was confirmed by co-culture. Unexpectedly, sprout LAB failed to suppress the growth of S. enterica in alfalfa sprouts, whereas all LAB strains stimulate S. enterica growth even if it is not significant in some strains. The findings of this study indicate that S. enterica-antagonistic LAB are detrimental to food hygiene and will contribute to further LAB research and improved vegetable sprout production.

Sweet pepper(paprika) belongs to the genus Capsicum, and is one of the most important export product from Korea to Japan and Southeast Asia. So it is important to eradicate plant quarantine pests before export sweet pepper. Aphids, whiteflies and mites are major pests that can damage to sweet peppers. Fumigation is normally used to eradicate pests in plant quarantine, but phytotoxicity may can be appeared that affect the quality of the product. Low-temperature treatment, one of the most popular physical treatment, can reduce crop damage to preserve product quality, but it takes long time to kill pests, which can cause quality degradation. In this study, phytotoxicity of fumigants, phosphine(PH3), ethyl formate(EF) and PH3+EF on sweet peppers was investigated to use as basic data for physicochemical treatment. When treated with more than 35 mg/L of EF, phytotoxicity was occurred, and was not occurred with PH3. When low-temperature of 1.7 degrees treated for 15 days after fumigation, it seems to be no direct damage from low-temperature treatment. But quality of top of sweet pepper was decreased from 7 days after fumigation.

The acrosome cap allows sperm to penetrate the egg membrane and produce male pronuclei within female chicken eggs, facilitating successful fertilization. Given this, it is important to establish practical methods for evaluating the integrity of the acrosome cap and thus the quality of the rooster’s sperm. There are several established methods for evaluating the acrosomes of mammalian sperm, but none of these methods are suitable for evaluating the acrosome status of rooster spermatozoa. Therefore, a simplified method for evaluating the rooster acrosome is needed. Here we evaluated the usefulness of CBB (coomassie brilliant blue) staining of the acrosome at concentrations of 0.04%, 0.08%, and 0.3% CBB solutions. Our data revealed a clear staining pattern for intact acrosome caps at 0.04% and 0.08% CBB but not at 0.3% CBB. This protocol revealed differences in acrosome integrity between fresh and frozen rooster sperm smears suggesting that CBB staining may facilitate easier semen evaluation in roosters. This protocol allows for the accurate differential staining of acrosome cap in rooster spermatozoa.

Exosomes are Nano-sized lipid vesicles secreted from mammalian cells containing diverse cellular materials such as proteins, lipids, and nucleotides. Multiple lines of evidence indicate that in saliva, exosomes and their contents such as microRNAs (miRNAs) mediate numerous cellular responses upon delivery to recipient cells. The objective of this study was to characterize the different expression profile of exosomal miRNAs in saliva samples, periodically isolated from a single periodontitis patient. Unstimulated saliva was collected from a single patient over time periods for managing periodontitis. MicroRNAs extracted from each phase were investigated for the expression of exosomal miRNAs. Salivary exosomal miRNAs were analyzed using Affymetrix miRNA arrays and prediction of target genes and pathways for its different expression performed using DIANA-mirPath, a web-based, computational tool. Following the delivery of miRNA mimics (hsa-miR-4487, -4532, and -7108-5p) into human gingival fibroblasts, the expression of pro-inflammatory cytokines and activation of the MAPK pathway were evaluated through RT-PCR and western blotting. In each phase, 13 and 43 miRNAs were found to be differently expressed (|FC| ≥ 2). Among these, hsa-miR-4487 (|FC|=9.292005) and hasmiR- 4532 (|FC|=18.322697) were highly up-regulated in the clinically severe phase, whereas hsa-miR-7108-5p (|FC|= 12.20601) was strongly up-regulated in the clinically mild phase. In addition, the overexpression of miRNA mimics in human gingival fibroblasts resulted in a significant induction of IL-6 mRNA expression and p38 phosphorylation. The findings of this study established alterations in salivary exosomal miRNAs which are dependent on the severity of periodontitis and may act as potential candidates for the treatment of oral inflammatory diseases.

This study investigated the fumigant activity of phosphine (PH3) on 2 kinds of mealybug (Pseudococcus longispinus and P. orchidicola (Hemiptera: Pseudococcidae)) adults and nymphs. All of the two mealybugs adults showed higher LCT99 values than nymphs, and P. longispinus showed higher tolerance than P. orchidicola in a 12 L desiccator. The absorption of phosphine on 13 nursery plants showed 12.2~41.5% difference depending on the plant. All of the mealybugs treated with phosphine 2 mg/L in 0.5 m3 fumigation chamber for 4 h showed 100% fumigant activity, except P. longispinus adult (approximately 90% at bottom part). However, when the exposure time was increased to 24 h, all of them showed 100% mortality. In the treatment of 10 m3 container, the 24 h treatment of phosphine showed 100% mortality to P. longispinus and P. orchidicola adults and nymphs. In all the experiments, no phytotoxicity of phosphine observed on 13 plants until 1 month after treatment.

A combination using phosphine (PH3) and ethyl formate (EF) was performed to compensate for the disadventages (long exposure time and phytotoxicity) of a single substance. P. longisipinus was more susceptible to mixed phosphine and ethyl formate than P. orchidicola in 12 L dessicator. Mortality of mixed treatment was higher than aggregated mortalities that treated individually, so it indicated to have a synergic effect on each other. Ethyl formate was showed higher adsorption rate than phosphine on imported nursery plants and showed differences depending on the plant. P. longispinus and P. orchidicola was showed 100% mortality when phosphine and ethyl formate was treated as 1 g/m3 and 30 g/m3 for 4 hour in 0.5 m3 fumigation chamber and in 10 m3 container. Phytotoxicity was evaluated to mixture of phosphine and ethyl formate at 1 month after treatment.

Background : Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz and Peucedanum japonicum Thumberg　are a major medicinal plant in north Geungbuk province. Using medicinal plants are impotant it`s ingredient. Dry condition and stroage method are not standard manual. The ingredient variation of dry condition and stroage method were not researched. Methods and Results : Using plant material were cutivated on Gyongsangbukdo Bonghwa area. It were studied ingredient variation after dry and storage condition by HPLC methods. Major ingredient of Angelica gigas Nakai are decurusin, decurusinangelate. Heated air bulk dry get more decursin than natuarl dry and decurusinangelate of natural bulk dry was higher than heated air bulk dry. Major ingredient of Ostericum koreanum Maximowicz are imperatorin and isoimperatorin.. Imperatorin of Ostericum koreanum was highest peak on 50℃ heated-air dry after plastic bag sorage and isoimperatorin was highest peak on 40℃ heated-air dry after mountain cultivation. Imperatorin is a major ingredient Angelica dahurica Bentham et Hooker. Heated air bulk dry get more decursin and decursinangelate than natuarl dry and small heated-air dry. Peucedanol-7o_glucoside is a major ingredient Peucedanum japonicum Thumberg. Natural bulk dry get more peucedanol-7o_glucoside than heated-air bulk dry. Conclusion : Ingredient of Angelica dahurica Bentham et Hooker, Angelica gigas Nakai, Ostericum koreanum Maximowicz are different under various cutivation, drying method, storage. Diffent Ingedients of Angelica gigas Nakai, Ostericum koreanum Maximowicz were not accord it’s optical conditon.

Background : Cylindrocarpon root rot, caused by Cylindrocarpon destructans and Fusarium solani, is a major disease which lead to replanting failure in ginseng garden. Chlamydospores of C. destructans, generated by poor environmental condition, can survived more than ten years in soil without host plant, ginseng. Density of soil pathogens gradually decreased as the progress of time since ginseng was harvested. Methods and Results : Soil chemical properties was analyzed from soil samples of 43 regions in farmer’s ginseng garden to study the variation of content and the correlation among inorganic contents. Soil samples of 24 regions was also analyzed to study correlation between progressed-year after ginseng harvest and soil chemical properties. Variation of soil chemical properties in descending order was Fe, Zn, P2O4, NO3, Mn. The content of Fe and Zn were great variation among inorganic chemicals of soil of farmer’s field. Electrical conductivity to induce physiological demage in excessive concentration showed highly significant positive correlation with the content of NO3 and K. As the progress of year after ginseng harvest, the content of organic matter and zinc was increased, while pH and Na were decreased in farmer’s field to cultivate ginseng. There were highly significant positive correlation between the progress of year after ginseng harvest and zinc content in farmer’s field to cultivate ginseng. Ratio of root rot of 2-year-old ginseng showed significant positive correlation with K content, and negative correlation in experimental field cultured by six rotation crops for one year. Conclusion : Root rot by soil pathogens was closely related with the content of potassium and zinc in soil chemical properties.

Background : Root diseases caused by Cylindrocarpon destructans and Fusarium solani decrease the yield and quality of ginseng. Cylindrocarpon root rot is a major disease caused by replanting failure in ginseng garden. Methods and Results : Solarization was done in the infested soil of the greenhouse for summer season (from July 24 to Autumn 31, 2014) after putting green manure (Sudan grass) and calcium cyanamide (CC) into the soil. Mycelium and conidia of C. destructans died at 4 0℃ after 15 hours, and 45℃ after 5 h, but it did not die at 35℃ after 15 h. Those of C. destructans died after keeping it for 2 hours daily at 40℃ for 9 days, and 45℃ for 8 days, but did not die at 38℃ for 9 days. Maximum soil temperature was 55.4℃ in 5 cm depth, 48.7℃ in 10 cm, 44.7℃ in 15 cm, 42.5℃ in 20 cm, and 31.9℃ in 30 cm by putting green manure into the soil and solarization. Reduction of sudan grass increased electrical conductivity (EC), organic matter, P2O5, K, and Mg, while decreased pH, NO3-N, and Na. Addition of calcium cyanamide and urea gave a negative effect on the growth of ginseng because EC and NO3-N were increased excessively than the optimal range. Solarization using green manure mixed with CC was the most effective in decreasing soil-borne pathogens of 2-year-old ginseng. But the root disease that occurred between single treatment of sudan grass and the treatment mixed with calcium cyanamide showed not a significant different. Addition of calcium cyanamide showed the decrease of root weight because leaves were dead early by a excessive increase of EC and NO3-N. Conclusion: Soil disinfection using green manure and solarization in greenhouse was effective in inhibiting root rot, however, it did not completely kill the soil-borne pathogens.

The aim of this study was to select the abiotic tolerant sorghum mutants using chlorophyll a transient OJIP analysis of PSⅠ and PSⅡ so called Kautsky’s effect within 1 second. It was clearly identified that wwt-and drought tolerant sorghum mutants could be classified by wet factor index(WFI). On the basis of WFI, wet tolerant sorghum matants were classified as follows; Ⅰ group, MUT534 bmr/new, MUT525 bmr; Ⅱ group, M2P1207 bmr, 25M2-0404 bmr, MUT371 bmr24, unknown bmr22, 10M2-0775 bmr, MUT135 bmr23; Ⅲ group, M2P0411 bmr, MUT641 bmr, M2P1064 bmr36, MUT855 bmr, 25M2-0137 bmr/new, MUT436 bmr, M2P0929 bmr, 25M2-0026 bmr, 10M2-0387 bmr, 25M2-0173 bmr/new; Ⅳ group, 25M2-0698 bmr. In conclusion, for the selection of wet tolerance, four photochemical parameters such as Electron transport flux until PSI acceptors per PSII(RE1o/RC), Performance index for energy conservation from photons absorbed by PSII antenna, until the reduction of PSI acceptors(PI_total ABS), Driving force on absorption basis(DF_total ABS) and Electron transport flux from QA to QB per PSII(ETo/RC) were important photochemical parameters deduced from maximum quantum yield and electron transport efficiency.

This study was carried out to determine the optimum harvest date of unshelled immature peanuts and dried kernels from 60 to 120 days after flowering. Fresh pod yield of spanish-type variety, 'Shaedl-tangkong(SD)' reached a peak of 9,140kg/ ha at 70 (days after flowering(Aug. 13) while that of virginia-type variety, 'Daepoongtangkong(DP)' reached a peak of 8,820kg/ha at 90 days(Sept. 11) after flowering. SD and DP showed maximum fresh kernel yield of 6,090 and 6,470kg/ha at 90 days after flowering (Sept. 11), respectively, while dry kernel yield reached a peak of 3,300 and 3,720kg/ha at 110 days(Oct. 1), respectively. Oil content of SD and DP were the highest at 90 days and 100 days after flowering, respectively and the oil content of two varieties increased rapidly from 60 to 90 days. Tannin content of the seed hull of SD increased continuously until 110 days after flowering while that of DP maximized at 100 days. The tannin content of the two varieties increased rapidly from 60 to 100 days. Total sugar of SD and DP showed highest content at 60 days and 70 days after flowering, respectively and suger content decreased very rapidly until 80 days and after that sugar content kept nearly constant. Oil, tannin and total sugar content of spanish-type SD were higher than those of virginia-type DP.