The blackish cicada, Cryptotympana atrata Fabricius, 1775 (Hemiptera: Cicadidae) was originally distributed mainly in the southernmost remote island, Jeju and rarely throughout low lands in South Korea, but has been explosively increased at the urban areas, where annual temperature is higher. In this study, we sequenced a partial mitochondrial COI from a total of 171 individuals collected throughout 12 localities in South Korea. The haplotype found with the highest frequency in Jeju island shares only with two inland localities in southern region with a low frequency, whereas the haplotype found with the highest frequency throughout inland localities was not found in Jeju island. These results showed that Jeju population, southern region, and other inland populations form somewhat different genetic groups.

The large copper butterfly Lycaena dispar (Haworth, 1803; Lepidoptera: Lycaenidae) has been categorized as a near-threatened species (NT) in South Korea from 2012 mainly due to limited distribution. In this study, we visited 36 sites spread across all South Korean provinces to verify the distributional range of the species and sequenced mitochondrial COI for 53 individuals from nine sites. We observed L. dispar at 15 sites in six provinces, including the two previously known provinces, indicating a southward range expansion. The in-field monitoring and genetic data collectively suggested that L. dispar does not have a limited distribution nor is it isolated, indicating that it should be reclassified as less vulnerable. Our study demonstrates that the combination of field and genetic data can provide a more reliable assessment of the stability of a species.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of the domesticated silkworm B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We sequenced 100 mitochondrial genomes of B. mandarina collected from South Korea and Japan and these were combined with public data. Phylogenetic and population genetic analyses showed that all individuals collected in South Korea, except one individual from Inje, formed a strong group together with northern China and some individuals of southern China. This group was placed as the sister group to B. mori strains, suggesting that this group could have been served as an immediate progenitor for B. mori. For further robust inference more analysis is underway.

Ceriagrion nipponicum (Odonata: Coenagrionidae) is listed as climate-sensitive indicator species in Korea and now expands its range northward. In this study, we sequenced complete mitochondrial genome (mitogenome) of the species collected from South Korea for comparatively analysis in damselflies and to detect the genes suitable for subsequent population genetic study. Comparison of the mitogenomes from two geographic samples of C. nipponicum showed the highest variation in ND4 and ND1, whereas no variation was detected in COI, warranting usefulness of the two genes for subsequent population-level study. Phylogenetic analysis using 13 PCGs and 2 rRNAs in Zygoptera showed non-monophyletic Coenagrionidae, forming two groups.

Bombyx mandarina (Lepidoptera: Bombycidae), the presumed ancestor of B. mori, has long been a subject of study to illustrate the geographic relationships in connection with origin of B. mori. We report 97 mitochondrial genome (mitogenome) sequences of B. mandarina collected from Korea and Japan. Phylogenetic and population genetic analyses showed that all individuals of B. mandarina collected in Korean localities formed a strong group together with all individuals originated from northern China (mainly north of the Qinling-Huaihe line) and some of southern China. This group was placed as the sister group to B. mori strians suggesting that this group had been served as an immediate progenitor for B. mori.

In Korea, both Sympetrum depressiusculum Sélys, 1841 (Odonata: Libellulidae) and Sympetrum frequens Sélys, 1883 are recorded. However, the identity of Korean populations and the validity of listing the two species have not yet been settled. In this study, we collected 74 individuals from Kroea, Russia, the Netherlands, and Japan. These were sequenced for COI, 16S rRNA, and ITS region. Major morphological characters and phylogenetic, network, and structure analyses all consistently suggest that Korean populations form a single species. Consequently, it could be valid to treat Korean populations as one species, S. depressiusculum, by applying the senior name.

Glutamine has been used to treat canine patients with parvoviral enteritis. However, little is known about the effect of L-alanyl-L-glutamine (Ala-Gln) supplementation in dogs with parvoviral enteritis. The objective of this study was to determine whether Ala-Gln supplementation can improve dog survival and ameliorate clinical signs without adverse effects. We conducted a randomized, double-blind, placebo-controlled clinical trial involving 39 client-owned dogs. The dogs were randomly assigned to two groups and administered either an Ala-Gln solution (Dipeptiven, 0.4 g/kg, n = 20) or an equivalent volume of placebo (n = 19) orally twice daily. Of the 39 dogs, 17 were vaccinated (n = 9 in the Ala-Gln-treated group and n – 9 in the placebo group). All dogs received standard treatment while hospitalized. The dogs were monitored according to a clinical scoring system and evaluated diagnostically daily for 11 days. Survival rates in both groups were quantified using Kaplan‒Meier survival curves and statistically compared using the log-rank test. The total score for clinical signs did not differ between the groups, except on day 2. The survival rates differed significantly (p=0.038). Three Ala-Gln-treated dogs (15.0%) died during the study, whereas eight dogs in the placebo group died (42.1%). No adverse effects were found to be associated with Ala-Gln treatment. Oral administration of Ala-Gln improves survival in dogs with parvoviral enteritis without causing adverse effects.

본 연구에서는 한국산 맵시벌과 3종 (운문점박이납작맵시벌, 잎말이나방살이뭉툭맵시벌, 안경꼬마자루맵시벌)의 새로운 숙주를 보고하고 자 한다. 새로운 숙주는 각각 자나방과, 잎말이나방과, 풀명나방과에 속하며, 이 중 자나방과와 풀명나방과는 각각 쌍점박이납작맵시벌속, 안경꼬 마자루맵시벌속의 숙주로써 처음으로 기록된다. 여기에 맵시벌과 3종의 간략한 특징과 우화된 표본의 사진, 숙주의 성충 사진을 제공하였다.

This study was conducted to identify changes of insect compositions and diversity after construction of an onshore wind farm. We investigated insect fauna and compositions between a grassland deforested by the construction and a forest located at Yeongdeok and Yeongyang, Gyeongsangbuk-do, Korea. Insects were collected using a sweeping net and light trap. A total of 11 orders, 50 families, and 246 species, and 1,076 individuals were collected at study sites. By taxonomic group, Lepidoptera species were the most frequently found with 141 species, 417 individuals (38.8%), followed by Hymenoptera (20.6%), Hemiptera (16.2%), and Orthoptera (12.3%). There were also significant differences in insect species and community compositions between sites. Creating open-field deforested forests are beneficial for some insect groups such as Hymenoptera and Orthoptera. Our results suggest that deforesting by the construction of an onshore wind farm might affect the composition and diversity of insects. Results of this study provide basic data for research on onshore wind farms.