The biodiesel production characteristics in a pulsed-corona plasma reactor has been investigated through parametric tests. Transesterification of rapeseed oil together with camelina oil was done with the change of such variables as voltage of power, molar ratio, KOH catalyst and temperature. The energetic electrons emitted from pulsed-corona plasma has contributed to the enhancement of yield on rapeseed oil in short time (15 min). The higher yield on camelina oil was observed in 5 min. The optimal parameters were shown as the voltage of 23 kV, the molar ratio of 5/1, the content of KOH catalyst of 0.6 wt% and the temperature of 28℃ under the rotating rate of spark gap of 900 rpm.

Black soybeans, which have been widely utilized as food and as material for Oriental medicine, contain anthocyanins in the seed coat. Soybean seeds with black seed coat and green cotyledon are called “Seoritae” in Korea. Korean customer prefer “Seoritae” to normal black soybean with yellow cotyledon. The pigments contained in green cotyledon of black soybeans were chlorophylls and lutein. These constituents function as antioxidant and protect humans against diverse damages. The purpose of this study was to investigate the most suitable extraction condition and method of green cotyledon pigments in black soybeans. The green cotyledon pigments were analyzed on RP-HPLC with C18 column using gradient system. The gradient system was used two mobile phases. A gradient elution was performed with mobile phase A, consisting of 100% MeOH, and mobile phase B, consisting of 100% EtOAc. Among the 4 kinds of extraction method using methanol and/or acetone solution, ultrasonic extraction at 30 min. using acetone solution was most suitable extraction condition for simultaneous analysis of chlorophylls and lutein in soybean with green cotyledon.