본 프로그램은 2007개정 초등학교 과학과 교육과정을 기본으로 원예 활동에 과학교육을 접목하여 아동의 스트레스를 감소시키고, 정서지능을 높이며 사회성을 향상시키고자 실시하였다. 그 결과 스트레스가 감소하였으며, 정서지능 증가에 효과가 있는 것으로 나타났다. 사회성에는 유의미한 차이를 보이지 않았지만, 교사의 관찰 일지를 통하여 관찰력과 탐구력 및 사회성 향상에 긍정적인 효과가 있는 것을 확인하였다. 또한 학부모 설문지 결과를 통해 어린이과학생물학교 프로그램이 과학교육과 원예활동을 접목하여 창의적이고 탐구적인 효과를 얻고자 하는 새로운 시도로 평가 되었으며, 이에 대한 학부모들의 기대가 높은 것을 알 수 있었고, 이 수업과정을 통해 자녀들이 지적인 습득과 함께 심리 정서적 안정감을 얻기 원하는 것으로 나타났다. 앞으로 원예와 여러 분야의 교과부분을 접목하여 통합적인 프로그램을 제시하고, 보다 심도 있는 원예활동을 실시하여 아동들의 성장 발전에 효과를 거둘 수 있도록 연구해야 할 것이다.

Manganese () is a trace element that is essential for normal physiology, and is predominantly obtained from food. Several lines of evidence, however, demonstrated that overexposure to exerts serious neurotoxicity, immunotoxicity and developmental toxicity, particularly in male. The present study aimed to evaluate the effect of 0, 1.0, 3.3, and 10 mg/kg/day doses of on the reproductive organs in the immature female rats. Rats (PND 22; S.D. strain) were exposed to () dissolved in drinking water for 2 weeks. The animals were sacrificed on PND 35, then the tissues were immediately removed and weighed. Histological studies were performed using the uteri tissue samples. Serum LH and FSH levels were measured with the specific ELISA kits. Body weights of the experimental group animals were not significantly different from those of control group animals. However, ovarian tissue weights in 1 mg and 3.3 mg dose groups were significantly lower than those of control animals (p<0.05 and p<0.01, respectively). Uterine tissue weights of 3.3 mg dose groups were significantly lower than those of control animals (p<0.01), while the 1 mg dose and 10 mg dose failed to induce any change in uterine weight. Similarly, only 3.3 mg dose could induce the significant decrease in the oviduct weight compared to the control group (p<0.05). Non-reproductive tissues such as adrenal and kidney failed to respond to all doses of exposure. The uterine histology revealed that the exposure could affect the myometrial cell proliferation particularly in 3.3 mg dose and 10mg dose group. Serum FSH levels were significantly decreased in 1mg dose and 10 mg groups (p<0.05 and p<0.01, respectively). In contrast, treatment with 1 mg dose induced a significant increment of serum LH level (p<0.05). The present study demonstrated that exposure is capable of inducing abnormal development of reproductive tissues, at least to some extent, and altered gonadotropin secretions in immature female rats. Combined with the well-defined actions of this metal on GnRH and prolactin secretion, one can suggest the might be a potential environmental mediator which is involved in the female pubertal process.