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        검색결과 17

        1.
        2023.10 구독 인증기관·개인회원 무료
        Honey bees are crucial pollinators for agricultural and natural ecosystems, but are experiencing heavy mortality in Korea due to a complex suite of factors. Extreme winter losses of honey bee colonies are a major threat to beekeeping but the combinations of factors underlying colony loss remain debatable. Finding solutions involves knowing the factors associated with high loss rates. To investigate whether loss rates are related to Varroa control and climate condition, we surveyed beekeepers in korea after wintering (2021–2022 to 2022–2023). The results show an average colony loss rate of 46%(2022) and 17%(2023), but over 40% colony loss before wintering at 2022. Beekeepers attempt to manage their honey bee colonies in ways that optimize colony health. Disentangling the impact of management from other variables affecting colony health is complicated by the diversity of practices used and difficulties handling typically complex and incomplete observational datasets. We propose a method to 1) Varroa mite population Control by several methods , and 2) Many nursing bee put in hive before wintering.
        4.
        2013.10 구독 인증기관·개인회원 무료
        Most traditional genome sequencing projects involving infectious viruses include culturing and purification of the virus. This can present difficulties as an analysis of multiple populations from multiple locations may be required to acquire sufficient amount of high-quality DNA for sequence analysis. The electrophoretic method provides a strategy whereby the genomic DNA sequences of the Korean isolate of Pieris rapae granulovirus (PiraGV-K) were analyzed by purifying it from host DNA by pulsed-field gel electrophoresis, thus simplifying sampling and labor time. The genomic DNA of infected P. rapae was embedded in agarose plugs, digested with a restriction nuclease and methylase, and pulsed-field gel electrophoresis (PFGE) was used to separate PiraGV-K DNA from the DNA of P. rapae, followed by mapping of fosmid clones of the separated viral DNA. The double-stranded circular genome of PiraGV-K encodes 120 open reading frames (ORFs), covering 92% of the sequenced genome. BLAST and ORF arrangement showed the presence of 78 homologs to other genes in the database. The mean overall amino acid identity of PiraGV-K ORFs was highest with the Chinese isolate of PiraGV (~99%), followed up with Choristoneura occidentalis ORFs at 58%. PiraGV-K ORFs were grouped, according to function, into 10 genes involved in transcription, 11 involved in replication, 25 structural protein genes, and 15 auxiliary genes. Genes for Chitinase (ORF 10) and cathepsin (ORF11), involved in the liquefaction of the host, were found in the genome. The recovery of PiraGV-K DNA genome by pulse-field electrophoretic separation from host genomic DNA had several advantages, compared with its isolation from particles harvested as virions or inclusions from the P. rapae host. We have sequenced and analyzed the 108,658 bp PiraGV-K genome purified by the pulsed field electrophoretic method. The method appears to be applicable to the analysis of genomes of large viruses. The chitinase, identified by PiraGV-K genome sequence, was functionally characterized by quantitative PCR, Western blot analysis, immunohistochemistry and transmission electron microscopy.
        5.
        2012.12 KCI 등재 서비스 종료(열람 제한)
        Studies in Modern Grammar 70, 107-127. It is known that VP-ellipsis and VP-anaphora phenomena are typologically dependent. The goals of this paper is to offer a unified algorithm for English VP-ellipsis and Korean VP-anaphora, and show how the suggested resolution algorithm, adopted from Jager (2010), can account for these two in a uniform way.
        8.
        2006.08 KCI 등재 서비스 종료(열람 제한)
        A new oat (Avena sativa L.) cultivar 'Darkhorse' was developed by Honam Agricultural Research Institute (HARI) m2005. It was released because ofits earlmess, high forage yield potential and adaptability for late summer sowing. It was devel-oped from the a
        9.
        2006.08 KCI 등재 서비스 종료(열람 제한)
        A new oat (Avena sativa L.) cultivar, "Highspeed" was developed by Honam Agricultural Research Institute (HARI) in2005. It was released because of its earliness, high forage yield potential, and good adaptability for late summer sowing. "High-speed" was s
        10.
        2004.10 KCI 등재 서비스 종료(열람 제한)
        A new vegetable peanut variety “Bakjung(Arachis hypogaea L.)” was developed from the cross between Shindaekwang and Suwon94 at the Honam Agricultural Research Institute and Yongnam Agricultural Research Institute, National Institute of Crop Science in 200
        11.
        2004.10 KCI 등재 서비스 종료(열람 제한)
        A new peanut variety “Kokwang(Arachis hypogaea L.)” was developed from the cross between Florigiant and Tifapan at the Honam Agricultural Research Institute in 2003. This variety has shinpung type and erect growth habit with midium size obovate dark green