The potential ability of Button mushroom compost (BMC) to solubilize heavy metals was estimated with metal contaminated soils collected from abandoned mines of Boryeong area in South Korea. The bacterial strains in BMC were isolated for investigating the mobilization of metals in soil or plant by the strains and identified according to 16S rRNA gene sequence analysis. When metal solubilization potential of BMC was assessed in a batch experiment, the BMC was found to be capable of solubilizing metals in the presence of metals (Co, Pb and Zn) and the results showed that inoculation of BMC could increase the concentrations of water soluble Co, Pb and Cd by 35, 25 and 45% respectively, than those of non-inoculated soils. BMC-assisted growth promotion and metal uptake in sunflower (Helianthus annuus) was also evaluated in a pot experiment. In comparison with non-inoculated seedlings, the inoculation led to increase the growth of H. annuus by 27, 25 and 28% respectively in Co, Pb and Zn contaminated soils. Moreover, enhanced accumulation of Co, Pb and Zn in the shoot and root systems was observed in inoculated plants, where metal translocation from root to the above-ground tissues was also found to be enhanced by the BMC. The apparent results suggested that the BMC could effectively be employed in enhancing phytoextraction of Co, Pb and Zn from contaminated soils.

A total of 35 phosphate solubilizing bacterial strains were isolated from waste mushroom bed of Agar-icus bisporus in Buyeo-Gun, Chungnam and screened for the production of indole acetic acid (IAA). The best IAAproducing strain was identified as Pantoea rodasii using 16S rRNA analysis. In addition to the IAA production, thisstrain could act as an efficient phosphate solubilizer (1100µg ml-1 after 5days of incubation) also. The selected strainwas cultured under different conditions in order to assess the optimum conditions for maximum IAA production. Thenutrient broth (NB) medium was recorded as the best medium, where the maximum IAA production (229µg ml-1)was recorded at the start of stationary phase (12hours after inoculation) of the bacteria growth. The performanceof the strain was found to be maximum at the temperature of 30℃ followed by 25℃. IAA production was foundto be increased with increasing tryptophan concentration (from 0.1 to 0.6%), however beyond this limit, a slight reduc-tion in IAA production was observed. The strains’ ability to produce IAA was further confirmed by extraction ofcrude IAA and subsequent TLC analysis. A specific spot from the extracted IAA preparation was found correspondingwith the standard spot of IAA with same Rf value. The results of HPLC analysis conducted in identifying and quan-tifying the IAA production more precisely, are in agreement with the results of the assessment done with colorimetricmethod. As revealed by the results of the pot experiment, the isolated strain could significantly enhance the growth(as measured by shoot and root growth) of mung bean plants compared to that of non-inoculated plants. Thereforeit can be concluded that the present strain, Pantoea rodasii has great potential to be used as bio-inoculants.

The degradation of concrete exposed to high temperature was evaluated by applying ultrasonic pulse velocity evaluation method which is one of the nondestructive tests of concrete. As a result, it is difficult to distinguish compressive strength of concrete and estimate residual compressive strength of concrete. However, it was confirmed that the higher the heating temperature, the lower the compressive strength and ultrasonic velocity of the concrete.

Concrete has been recognized as a material which is resistant to the high temperature, but chemicophysical property of concrete is changed by the high temperature. So, mechanical properties of concrete may be reduced. Therefore, concrete is evaluated mechanical properties for safety inspection. However, research of ultrasonic pulse method is not much. Therefore, the purpose of this study is to Non-Destructive Test of 30, 70, 110MPa concrete exposed high temperature using ultrasonic pulse velocity.

Concrete has been recognized as a material which is resistant to the high temperature, but chemicophysical property of concrete is changed by the high temperature. So, mechanical properties of concrete may be reduced. Therefore, concrete is evaluated mechanical properties for safety inspection. However, research of ultrasonic pulse method is not much. Therefore, the purpose of this study is to Non-Destructive Test of 30, 70, 110MPa concrete exposed high temperature using ultrasonic pulse velocity.

In this study, local fracture of concrete was evaluated through high-velocity impact test. As results, increase of bending tension performance by fiber reinforcement did not affect penetration depth Control, but it decreased rear side fracture thickness and diameter. Therefore, it was observed that fiber reinforcement decreases scabbing limit thickness.