This study was aimed to isolate bacterial inoculants producing chitinase and evaluate their application effects on corn silage. Four corn silages were collected from four beef cattle farms to serve as the sources of bacterial inoculants. All isolates were tested against Fusarium graminearum head blight fungus MHGNU F132 to confirm their antifungal effects. The enzyme activities (carboxylesterase and chitinase) were also measured to isolate the bacterial inoculant. Based on the activities of anti-head blight fungus, carboxylesterase, and chitinase, L. buchneri L11-1 and L. paracasei L9-3 were subjected to silage production. Corn forage (cv. Gwangpyeongok) was ensiled into a 10 L mini silo (5 kg) in quadruplication for 90 days. A 2 × 2 factorial design consists of F. graminearum contamination at 1.0104 cfu/g (UCT (no contamination) vs. CT (contamination)) and inoculant application at 2.1 × 105 cfu/g (CON (no inoculant) vs. INO (inoculant)) used in this study. After 90 days of ensiling, the contents of CP, NDF, and ADF increased (p<0.05) by F. graminearum contamination, while IVDMD, acetate, and aerobic stability decreased (p<0.05). Meanwhile, aerobic stability decreased (p<0.05) by inoculant application. There were interaction effects (p<0.05) on IVNDFD, NH3-N, LAB, and yeast, which were highest in UCT-INO, UCT-CON, CT-INO, and CT-CON & INO, respectively. In conclusion, this study found that mold contamination could negatively impact silage quality, but isolated inoculants had limited effects on IVNDFD and yeast.

This study was carried out to investigate insect community structure from different habitats in Baengnyeong island.We performed day and night collection at two different habitats (mountain, rural area) of Baengnyeong island from Mayto September in 2015. A total of 2,879 individuals of 404 species, 81 families belonging to 10 orders were collectedand identified. A dominant species was Idisia ornata Pascoe (Tenebrionidae) despite a very low percentage (6.04%) ofthe species among the catches. Results of independent t-test showed a significant high (p<0.001) of species richness onmountain. Also, seasonal results of ANOVA (Analysis of variance) were significantly influential with species abundanceand species richness. The result of NMDS analysis showed that the community structure of the insects from the mountainis different with rural area.

In the present study, we analyzed ecological functional groups of diverse hymenopteran families from five different vegetation stands in the Gwangneung forest. Hymenopterans were collected using three different traps from March to October 2015. Pollinators were a dominant group from March to May and declined significantly after that period. Parasitic group made major peaks from April to May and made another small peak in August. Predatory group reached to a peak in April and July respectively but appeared to be active even in October when all the other groups mostly disappeared. The vegetative communities were ordinated with the hymenopteran family assemblages using the Nonmetric multidimensional scaling (NMDS). The result of NMDS analysis showed that the community structure of the hymenopteran functional groups from the Quercus rubra stand is different from those of the other stands.

The genus Miaenia Pascoe, 1864 (Coleoptera Cerambycidae) in Korea is taxonomically reviewed in this study. Miaenia was erected by Pascoe (1864) based on the type species M. marmore. Miaenia has been accepted as a subgenus of Sciades for a long time, howerer, Roguet (2012) considered Sciades as a synonym of the genus Miaenia. Previously, two species, M. fasciata fasciata (Matsushita, 1943) and M. maritima Tsherepanov, 1979, of the genus have been reported in South Korea. In this paper, M. tonsa (Bates) is recorded in Korea for the first time. A key to the Korean Miaenia species, diagnoses, illustrations of diagnostic including male genitalia are provided.

A predatory genus Cryptalaus Ôhira, 1967 (Coleoptera: Elateridae) in Korea is taxonomically reviewed in this study. Up to date, two species of the genus Cryptalaus, C. berus Candèze, 1865 and C. larvatus pini Lewis, 1894, have been reported from Korea. From the present study, C. yamato Nakane, 1957 which has been regarded as an endemic to Japan, is reported from the Korean peninsula for the first time. A key to the Korean species of the genus Cryptalaus, diagnosis, illustrations of diagnostic characteristics are provided.

In the present study, Cydia kamijoi Oku is newly recognized in korean insect fauna. This tortricid moth was first found damaging the cones of Abies koreana in Jeju Island 2014. The moth can be a serious insect pest on A. koreana because of high damage rate on the cones, up to 71% average. The genus Cydia now was 11 korean species including C. kamijoi. Regarding this species, some basic information such as collection records, morphological characters, and ecology were provided

Pogonini Laporte, 1834 is one of the tribes in Trechinae Bonelli, 1810 (Coleoptera: Carabidae). This tribe is morphologically similar to Trechini, Benbidiini, Zolini and Patrobini of the subfamily. However, Pogonini can be distinguished from the other tribes by having a combination of the following characters: apical maxillary palpomere fusiform; umbilical series of setae on elytron more or less contiguous (though sparse in middle); each tarsomeres with a median sulcus on dorsal side. Pogonini includes about 85 species of 12 genera worldwide and is found in all zoogeographical regions of the world. Many species inhabit along the sea coasts or near salt lakes, and are recognized as halobiont. In Korea, Paik (1998) reported Pogonus (Pogonus) itoshimaensis Habu, 1954. However, he added only the species name in the checklist without description or illustration although few specimens were examined. After Paik’s study, any studies on taxonomic status and distribution of Korean Pogonini have not been carried out. The genus Pogonus Dejean is the largest group in Pogonini with two subgenera: P. (Pogonoidius Carret, 1903) and P. (Pogonus Dejean, 1821). These two subgenera share very similar external morphological characters; Pogonoidius has setae on anterior angles of pronotum and setae at 7th interval of elytra while Pogonus does not have them. In the present study, two species, Pogonus (Pogonus) japonicus Putzeys, 1875 and P. (Pogonoidius) punctulatus Dejean, 1828, are newly recognized from Korea. A key to the Korean Pogonini species, redescription, distribution, photos of diagnostic characters, and illustrations of male and female genitalia are provided.

To date there have been only two species in genus Pogonus and subgenus Europhilus of genus Agonum recognized from Korean insect fauna: Pogonus itoshimaensis Habu, 1954 and Agonum (Europhilus) bellicum Lutshnik, 1934. In the present study one additional species for each genus is newly recognized from Korea: Pogonus (Pogonus) japonicus Putzeys, 1875 and Agonum (Europhilus) gratiosum nipponicum Habu, 1972. A key to adults, redescription, diagnostic photos of adult and male and female genitalia are provided.

Insect pollinators of the endanger orchid Cypripedium japonicum were surveyed and identified during two years, as a part of a conservation project of the orchid at Jukyeup-san and Hwaak-san (Mt.), South Korea. In total 40 individuals of 16 species in 4 families were identified. The dominant family was Halictidae, and Lasioglossum exiliceps Vachal visited the most frequently C. japonicum during the surveys. The average visiting frequency was 2.5 individuals per hour and the highest 4.3, from 12:00 – 13:00 in a day. After 15:00 insects did not visit the flowers at all. However, all of the visiting insects were found to not carry a pollinium or pollens of the orchard on their bodies; pollen carryover by any of the visiting insects did not occur at all. The orchid seems to require certain pollinators in particular body thickness due to its unique pollination mechanism. The orchid has two exit route openings, around 1 cm in diametre, where the entrapped insects can exit and an anther is situated just in front of each opening. It was inferred that a pollen carrier should be around 1 cm in body thickness. Therefore, the candidate species as the proper pollen carriers can be Tetralonia nipponensis Perez, Xylocopa appendiculata circumvolans Smith and Bombus consobrinus Dahlbom among the surveyed visitors.

Caloptilia Hübner, 1825 is one of the largest group in Gracillariidae. 314 species have been known around the world. To date, 15 species were known from Korea. In the present study, four species of Caloptilia are newly recognized from Korean insect fauna: C. fidella (Reutti), C. hidakensis Kumata, C. illicii Kumata, and C. pulverea Kumata. All of 19 Korean species, including four newly recognized species, are redescribed with illustrations of adults and genitalic structures.

Microbial fermented cellulose gel, citrus gel(CG), was successfully fabricated to porousfoam by radiation treatment and freeze drying. The chemically induced radiation was used to createhighly porous foam and further freeze drying of the CG produced tough foams with interconnectedopen pores for use in tissue engineering. The microstructure of the CG foam was controlled byvarying the irradiation dose and quenching temperature with pore size ranging from several micronsto a few hundred microns. Tensile strength and Gurley value of the CG foam were influenced byirradiation dose. These radiation induced CG foams are promising scaffolds for tissue engineering.

To study the signaling effect of insulin-like growth factor-Ⅰ(IGF-1), transgenic mice containing IGF-1 Receptor (IGF-1R) cDNA fused to metallothionein promoter were produced by DNA microinjection into the pronucleus of mouse zygote. Three founders were produced with transgenic mice containing IGF-1R gene. Transgenic mice lines contained approximately 4～20 copies of transgenes per cell and transmission of this gene into the progeny with Mendelian manner were determined. The founder mice were mated with normal mice to produce F1 mice and then F2 mice. Transmission rates of IGF-1R transgene in the progeny mice were 25～60% in F1 generation and 40～50% in F2 generation. The mRNA expression of IGF-1R transgene in liver was analyzed using RT-PCR for IGF-1R gene in liver. When body weights of transgenic pups were measured during 4, 10 and 14 weeks after birth, IGF-1R transgenic mice grew faster than non transgenic littermates. This study indicated that growth regulation by IGF-1 signaling through IGF-1R can be elucidated using IGF-1R transgenic mice.