Immuno-MemBlot is a technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular or slot templates directly onto the membrane. Recently we developed a new Immuno-MemBlot (IMB) method applying immunoreactions and coloring procedures directly in the wells of MemBlot apparatus, which were connected by canals to perform drainage for reagent application and buffer irrigation. This IMB method was designed to get theimmunoblot results more rapidly and clearly than the previous immunoblot ones. This study is aimed to evaluate the analytical accuracy of IMB using different biological assay. In the sensitivity test of IMB the monoclonal antibody can clearly detect the 30 ng (about 12 pM) of Mucocidin peptide (35 mer), and is also available to detect at least 10 ng (about 4 pM) of Mucocidin peptide (35 mer). The IMB was effective in the quantitative analysis of methothrexate (MTX) assay for cellular apoptosis. And more, this IMB is useful to screen large number of specific samples with ease and accuracy in a short time. In the screenings for the presence of Mucocidin in saliva the quantitative comparison is conspicuous among 48 persons depend on the different conditions ofgender, drinking and smoking habits, and oral diseases. Therefore, it is presumed that, even though the target proteins were partly degraded, a specific epitope can be detected if a monoclonal antibody was still reactive. Conclusively, these data suggest that the IMB can be useful in the primary qualitativeand quantitative analysis of proteins in various fluids, i.e., blood, saliva, tear, urine, etc.

This study was aimed to behaviour of organics and nitrogen on the upflow anaerobic reactor when a acid fermenter is added. Upflow anaerobic reactor (UAR) reaction will result which operates, COD removal efficiencies of reactor with nitrate loading rate 0.11, 0.66g/L/d were over 77%, but one with 1.0g/L/d was 73.5%. Especially, on NLR 0.11g/L/d, COD removal was 77% and nitrate removal efficiency was 93% simultaneously. The other side upflow anaerobic reactor and acid fermenter (UAR+AF) reaction will result witch operates, COD removal efficiencies of reactor with nitrate loading rate 0.11, 0.66g/L/d were over 85%, but one with 1.0g/L/d was 80%. Especially, on NLR 0.11g/L/d, COD removal was 85% and nitrate removal efficiency was 98% simultaneously. Also, without in reaction condition increase of influent nitrate concentration resulted in the linear decrease of nitrate removal efficiency and nitrate removal efficiency at influent nitrate-nitrogen 800mg/L was 50%. Alkalinity was increased theoretically by denitrification at low nitrate-N concentration, however, it was not increased theoretically at high nitrate-N. 40% nitrate-N of UAR was denitrified until 70% height of reactor and 90% nitrate-N of UAR+AF was denitrified until 30% height of reactor Upflow anaerobic reactor was to occur accumulate acid, which TVA/Alkalinity is 0.3～0.47. Increase of NLR resulted increase of effluent alkalinity and TVA production