The invasive black soldier fly, Hermetia illucens, has been paid much attention as an excellent organic matter decomposer. We conducted the nationwide survey and the population genetic study using a mitochondrial cytochrome C oxidase 1 gene to understand its genetic diversity and distribution pattern in Korea. The results show that it has successfully settled down in South Korea and there are only 10 haplotypes and the populations of the insect are highly differentiated. The results indicate that only few maternal lineages were introduced and their dispersal was restrained due to their short distance flying tendency since their introduction.

This study was conducted to investigated the distribution and ecological character of Black Soldier Fly(BSF), Hermetia illucens, to determine effects of BSF on composing ability to waste-food. The distribution of BSF was defined in all parts of the country in Korea. Its main habitat was found to be areas near cattle sheds, manure sheds, living waste dump grounds, and food waste dump grounds. Observed characteristics of BSF by developmental stage may be summarized as follows: eggs were a long oval shape of 886.9±19.7 ㎛ in major axis and 190.1±9.7 ㎛ in minor axis; they were 24.0±1.6 ㎍ in weight. One adult insect laid 1001±247 eggs in quantity; days to hatch from eggs (27℃, 60% R.H.) were 81.3±12.5 hours. Larvae which were hatched appeared to be close to white and turned into pale yellow as being last instar larva. Last instar larva ranged from 20.7±1.1 mm in size, the length of larval stage was approximately 15～20 days. Pupae exhibited red brown, 19.2±1.1 mm in size; pupal state lasted 15.5±1.4 days for female, 14.7±1.4 days for male, exhibiting the tendency of males having shorter period than females. Adult insects were sized about 13～20 mm and colored black.

The time-zone of pollinating activity according to numbers of Apis mellifera hive released in the strawberry(Maehyang var.) houses was together from 9A.M. to 4P.M., and the peak times of pollinating activity were between 11A.M and 1P.M.. The effects on pollinating activity according to numbers of A .mellifera hive released in the strawberry houses were ordered 5bee combs(11,000heads), 4bee combs(8,800heads) and 3bee combs(6,600heads). The rate of workers lost in A. mellifera hives with 5bee combs and 4bee combs during the strawberry cultivating period were lower than that of 3bee combs. The rates of fruit set by pollinating activity according to numbers of A.mellifera hive released in the strawberry houses were same level with 99%. The fruit qualities; No. of seeds, sugar content and rate of normal fruit set were same level, but fruit weight was ordered 5bee combs in 16.9g, 4bee combs in 16.4g and 3bee combs in 15.6g. The rate of marketable fruit of 4bee combs and 5bee combs were 5% to 9% higher than that of 3bee combs, respectively.

The time-zone of pollinating activity relative to numbers released of Apis mellifera in the strawberry(Janghui var.) houses was together from 9A.M. to 4P. M., and the peak time of pollinating activity was 1P.M.. The effects on pollinating activity relative to the comb numbers in the honeybee hive released in the strawberry houses were ordered 5bee combs(11,000heads), 3bee combs(6,600heads) and 4bee combs(8,800heads). The rate of workers lost in A. mellifera hives with 5bee combs during the strawberry cultivating period were lower than those of 3bee combs and 4bee combs. The rates of fruit set by pollinating activity relative to the comb numbers in the honeybee hive released in the strawberry houses were same level with over 98%. The fruit qualities; number of seeds, sugar content and rate of normal fruit set were same level, but fruit weights was recorded 5bee combs with 40.8g, and 4bee combs and 3bee combs were showed with 37.8g. The marketing income of 5bee combs was 8% higher than that of 4bee combs and of 3bee combs, respectively.

The time-zone of pollinating activity relative to numbers released of Apis mellifera in the strawberry(Seolhyang var.) houses was together from 9A.M. to 4P. M., and the peak time of pollinating activity was 11A.M.. The effects on pollinating activity relative to the comb number in the honeybee hive released at the strawberry houses were ordered 5bee combs(11,000heads), 4bee combs (8,800heads) and 3bee combs (6,600heads). The rate of workers lost in A. mellifera hives with 5bee combs and 4bee combs during the strawberry cultivating period were lower than that of 3bee combs. The rates of fruit set by pollinating activity relative to the comb number in the honeybee hive released at the strawberry houses were same level with over 98%. The fruit qualities; No. of seeds, sugar content and rate of normal fruit set were same level, but fruit weights were ordered 5bee combs in 37.2g, 4bee combs in 35.6g and 3bee combs in 32.6g. The marketing incomes of 4bee combs and 5bee combs were 9% to 13% higher than that of 3bee combs, respectively.

The black soldier fly (Hermetia illucens) (Diptera: Stratiomyidae) is an invasive species that is thought to be endemic in Korea. The insect has been paid much attention thanks to its ability to decompose organic wastes. We collected the insect nation-wide in 2006 and 2010 to investigate its genetic structure based on the mitochondrial cytochrome oxidase 1 (mt CO1) haplotype. The results show that during the time period the insect seems to lose some rare haplotypes. We conclude that the insect may have experienced genetic bottleneck while its settlement in Korea. In our presentation, we show its updated haplotype network and genetic differentiation and propose potential explanations.

Bombus terrestris was released for pollination of apple flowers at apple orchards in Uileumgol, Milyang-sity, mid spring, 2008. Numbers of out-going bees and in-coming bees were 38heads at 10 a.m. and 23heads at 4 p.m., respectively. 75% bees per total in-coming bees collected the pollens about 10 a.m. and 63% bees per total in-coming bees collected the pollens around 4 p.m.. The SEM photography(A) showed that 82.7% of a pollen load were collected on apple flowers and 17.3% of a pollen load were collected from another plants and trees flowers. The another SEM photography(B) showed that 89.1% of a pollen load were collected on apple flowers and 10.9% of a pollen load were collected from another plants and trees flowers. Therefore these results show that B. terrestris mainly pollinates on the apple flowers but the bee seems to visit flowers of other plant species from time to time.

A multiplex polymerase chain reaction (PCR) was developed for the simultaneous detection and differentiation among Nosema apis and Nosema ceranae in honeybee. Three sets of primers were selected from different genomic sequences to specifically amplify a 831 bp amplicon within the SSU rRNA gene, specific for both N. apis and N. ceranae (MSSR primer); a 375 bp amplicon within the SSU rRNA gene, specific for N. apis (NA primer); and a 1,131 bp amplicon within SSU rRNA gene, specific for N. ceranae (NC primer). Using the primers in conjunction (multiplex PCR) we were able to N. apis and N. ceranae and to differentiate between them. The sensitivity of this PCR assay was approximately 102 spores per milliliter. We proposed that the multiplex PCR was sensitive, specific and rapid tool that can serve as a useful differential diagnostic tool for detecting N. apis and N. ceranae in honeybee.

This study was conducted to investigate the distribution pattern, ecological characteristics and life cycle of the Black Soldier Fly (Hermetia illucens, BSF). The BSF was widely distributed throughout Korea. The insect was mainly found in the vicinity of and in cattle sheds, manure sheds, living waste dump grounds, and food waste dump grounds. Developmental characteristics of the BSF are as follows: the egg was long oval shaped of 887㎛ in the major axis and 190㎛ in the minor axis; it weighed 24㎍. Female oviposited ca. 1,000 eggs on average; eggs hatched in 81 hours under laboratory condition (27℃, 60% R.H.). The duration of the larval stage was approximately 15-20 days. The size of the last instar larvae was 21mm. The cuticle of the pupae gradually acquired red-brown color and the size of them was 19 mm. The pupal stage was shorter for females (16 days) than males (15 days). Adults were sized about 13-20mm long and black-colored. The life span of adult insects was 5-8 days for the first generation (June-July), 7-10 days for the second generation (Aug.-Sept.), and 13-18 days for the third generation (Sept.-Oct.). Mating started on the next day of emergence and actively occurred at the third day after emergence. Mating mostly occurred between 10:00 and 16:00 during which light intensity is highest. Egg-laying started on the third day and was most frequent from the fourth to the sixth day after emergence. Similar to mating time, females oviposited mostly between 10:00 and 16:00.