A centrifugal cyclone dust collecting apparatus includes a hydro cyclone dust collecting apparatus for separating solid or liquid using liquid or suspension as a medium. In this study, the formation mechanism and improvement of air core and inner air layer were confirmed through Particle Image Velocimetry. These results showed that the modified experimental model was designed in the conventional method suitable for the separation of juvenile fish and eggs. The inlet speed of the multi-stage hydrocyclone dust collector, which can increase the inlet velocity and minimize floatage in the turbulence chamber, was increased from 0.15 to 0.30 m/s. As a result, the air core was stably formed, the inner air layer was increased with increasing speed. In addition, the dust collecting efficiency of egg and juvenile fish was 97.8% on average, It can infer that this system confirmed the ability to efficiently collect particles of 40 μm or more.

This study was conducted to understand the relationships between fish and zooplankton of distribution in the coastal waters of the East Sea from May to August 2016 using hydroacoustic. To distinguish between fish and zooplankton, we used the time varied threshold (TVT) method at the frequency of 120 kHz. As a result, the mean nautical area scattering coefficient (NASC) of fish was highest at 913 m2/n.mile2 in June and lowest at 315 m2/n.mile2 in July. The mean NASC of zooplankton was highest at 247 m2/n.mile2 in May and lowest at 70 m2/n.mile2 in July. The mean NASC of fish and zooplankton showed a significant difference (P < 0.05) with high correlation (R2 = 0.84). In addition, there was no significant difference in the mean NASC of fishes and zooplankton by depth (t-test, person correction = -0.17, p > 0.05).

Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at 4ºC. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.