Effect of the liquid circulation velocity on the biofilm development was investigated in an inverse fluidized bed biofilm reactor(IFBBR). To observe the effect of the influent COD concentration on biofilm simultaneously, the influent COD value was adjusted to 1000㎎/ℓ for 1st reactor, and 2500㎎/ℓ for 2nd reactor. The liquid circulation velocity was adjusted by controlling the initial liquid height. As the liquid circulation velocity was decreased, the settling amount of biomass was increased and the amount of effluent biomass was decreased. Since the friction of liquid was decreased by the decrease of liquid circulation velocity, the biofilm thickness was increased and the biofilm dry density was decreased. In the 1st reactor, the SCOD removal efficiency was constant regardless of the variation of the liquid circulation velocity, but it was increased by the decrease of the liquid circulation velocity because of more biomass population in 2nd reactor.