This study was conducted to investigate the effects of Woohwangcheungsimweun (ox bezoar), deer antlers, and wild ginseng on induction of cardiomyocyte differentiation using the established mouse embryonic stem (ES) cells. The expression of atrial natriuretic peptide (ANP) was highest in Woohwangcheungsimweun treatment group. The expression of rabbit anti-GATA-4(GATA-4) and troponin (TnI) were highest in wild ginseng and Woohwangcheungsimweun treatment groups, respectively. Fluorescence activated cell sorting (FACS) analysis showed that the expression of ANP was highest in Dimethyl sulfoxide(DMSO) and Woohwangcheungsimweun treatment groups. The expression of GATA-4 was relatively high in wild ginseng treatment group. The expression of TnI was highest in Woohwangcheungsimweun treatment group. In the gene expression analysis, DMSO greatly inhibited GATA-4 expression to 25% of control. Woohwangcheungsimweun treatment caused to increase cTnI and cardiac ANP expression significantly. Wild ginseng extract upregulated GATA-4 gene expression. In conclusion, DMSO widely used as cardiomyocyte differentiation inducer did not show significant effects on the expression of ANP, GATA-4 and TnI in this study. Woohwangcheungsimweun showed upregulation of ANP and TnI expression. Wild ginseng extract showed greater effects than DMSO on GATA-4 expression. These results might suggest that the combination of Woohwangcheungsimweun and wild ginseng extract treatment can be expected to increase expressions of all three genes.

시판 19종 한약재 methanol 추출물의 어병세균 Edwardsiella tarda에 대한 항균활성을 측정한 결과, 오배자 추출물에서 가장 강한 활성이 나타났다. 오배자 methanol 추출물은 용매 극성에 따라 분획하였으며, 각 분획물의 항균활성 측정 결과 ethyl acetate 층에서 가장 강한 항균 활성이 나타났다. 이는 E. coli와E. tarda 모두에 대해 유사한 결과를 보였다. 오배자 추출물의 최소생육저해농도(MIC)를 측정한

This study was investigated that quality inspection of distributed Scutellariae Radix in Korea. To evaluate the quality of these herbal medicines, we carried out TLC pattern, foreign matter in purity, loss on drying, ash, acid-insoluble ash, oil content, dilute ethanol-soluble, water-soluble, ether-soluble extracts contents, quantitative analyses, residual SO2, individual heavy metals and organochlorine pesticides. To measure contents of baicalin, baicalein and wogonin, we were quantitative analyzed using HPLC. The average contents of baicalin, baicalein and wogonin were detected by 13.28 (±0.43)%, 1.17 (±0.04)% and 0.40 (±0.02)%, respectively. Each average contents As, Cd, Hg and Pb was 0.059 (±0.054) mg · kg-1, 0.019 (±0.010) mg · kg-1, 0.017 (±0.057) ppm and 0.242 (±0.084) mg · kg-1, respectively.

The experiment was conducted to determine the effect of the mixed herbal medicine for the substitution of antibiotics on the performance of laying hens. Day old hyline 1,500 layer chicks were randomly assigned to 4 treatments. Control were 600 and three each treatment was 300. Eggs were collected at every weeks for measuring egg production and week 26, 27, 28 for chemical analyses. Chemical analyses were done for fatty acid profile of egg yolks, amino acid, antibiotics residue in eggs(collect eggs after supplying OTC 21ppm/bird/day to control for four day at 26, 27, 28) during the laying period(19～77 wk). On 0～4 week, T-3 showed higher feed intake than those fed the other diets and on 5～13 week, T-2 showed highest feed intake among treatments. After birds were moved (14～18 wk) CT-3 showed highest, but for overall rearing period, there was not significantly different among treatments. In conclusion the mixed herbal medicine did not tended to influence palatability to rearing birds. Egg quality(egg yolk color, haugh unit, eggshell breaking strength) showed no difference among treatments. Ratio of unsaturated fatty acid (mg/100g)/saturated fatty acid (mg/100 g) was not difference among treatments, but linolenic acid and docosahexaenoic acid(DHA) of the mixed herbal medicine treatments showed higher then control. The antibiotics residue of CT, T treatments egg was not detected and control was not detected or below allowance. In conclusion the mixed herbal medicine can be possible to feed laying hen without antibiotics.

The experiment was conducted to determine effect of the mixed herbal medicine for the substitution of antibiotics on the performance of laying hens. Day old hyline 1,500 layer chicks were randomly assigned to 4 treatments. Control were 600 chicks and there were three treatments of each 300 chicks. The 4 treatments were as follows: the mixed herbal medicine 0.1%(T-1), 0.3%(T-2) and 0.5%(T-3) after removed antibiotics on commercial feed and commercial feed(control) from 5th week to 13th week, each treatment was replicated 3 times and from 14th week to 18th week. Each 100 hens of the mixed herbal medicine 0.1%(CT-1), 0.3%(CT-2) and 0.5%(CT-3) were moved to cage. Body weight were measured on 4th, 8th, 13th week and feed intake, mortality were measured on every weeks. Body weight at fourth week, all treatments tended to be higher than control and T-3 statistically was highest(p<0.01). On 8th week, also treatments statistically high and T-1 was highest(p<0.01). But 12th week, there was not significantly different among treatments. Therefore it will be possible that the mixed herbal medicine substitute for antibiotics after vaccination. Mortality was not different between treatment and control overall rearing period. Early laying period(19～41wk), T-2 showed highest feed intake(107.1g) among treatments, later laying period(42～77wk), T-1 showed highest feed intake(134.3g) and was not any different among each treatments.

To develop optimal storage method of root of Anemarrhena Rhizome, which has been grown as major cultural herbal medicine material, root of Anemarrhena Rhizome was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The rot ratio of root during the storage period was not significantly different among packing materials but was significantly different among sealing conditions. Conclusively vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Anemarrhena Rhizome.

To develop optimal storage method of root of Zingiberis Rhizoma, which has been grown as major cultural herbal medicine materials, root of Zingiberis Rhizoma, was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusionally, vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Zingiberis Rhizoma.

To develop optimal storage method of root of Peucedani Radix, which has been grown as major cultural herbal medicine material, root of Peucedani Radix was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusively vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Peucedani Radix.

To develop optimumal storage method of root of Bupleurum falcatum Radix grown on major cultural herbal medicine materials, root of Bupleurum falcatum Radix was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusionally, vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Bupleurum falcatum Radix.

This study was conducted to investigate desirable storage method to major chinese herbal medicine materials, Moutan Radicis Cortex in different packing materials and sealing degree. The loss in weight of Moutan Radicis Cortex as influenced by packing materials was the lowest at complete sealing treatment with transparent or black polyethylene film. The rot ratio of Moutan Radicis Cortex during the storage period was not difference between packing materials, transparent and black polyethylene film, but rot ratio of complete sealing condition was lower than punch (ΦJ5mm) on polyethylene film.