For the destruction of toxic chlorinated organic compounds, this study proposes improved supercritical water oxidation method (multistep oxidation) using sodium nitrate as an oxidizer. This method solves the problems involved in the existing supercritical water oxidation method. Multistep oxidation means that NaNO3 is oxidized to N2 via NaNO2 and NO. Toxic and hard to destroy organic substances like para-dichlorobenzen(4-DCBz), polychlorinate biphenyl(PCB) ware oxidized to non toxic substances in a condition, in which rapid pressure and temperature rise is restrained as much as possible. 4-dichlorobenzene(4-DCBz) and Polychlorinate biphenyl(PCB) in condition(450℃, pw=0.25g/cm3, 30min) Was discomposed perfectly.

In an effort to uncover the spermatogenic impairment by the polychlorinated biphenyls (PCBs), the expression of tight junctions (TJs) genes important for the formation of the blood testis barrier (BTB) were examined following the 3,3',4,4',5-pentachloro biphenyl (PCB126) treatment in cultured neonatal testis in mice. At 4 days (D4) after 10 and 100 nM PCB126 treatment the expression of claudin-11 was significantly increased when compared with vehicle control. In contrast no difference in occludin and claudin-1 expression was found among the experimental group. On D8, 100 nM PCB126 significantly increased the expression of claudin-11 but not occludin and claudin-1. 1 uM PCB126 treatment significantly decreased expressions of occludin and ciaudin -1, suggesting the general toxic effect on the Sertoli cell. Because PCB126 does not alter the proliferative activity of spermatogenic cells and Sertoli cells in neonatal testis, it is likely that increase in the expression of claudin-11 by low dose of PCB126 may attribute to the alteration of the Sertoli cells differentiation in testis. It also emphasized that PCB126 might have differentially affected the transcription of TJ genes in Sertoli cells. In conclusion, this result suggests that the structure of TJ may be targeted by PCB126 in neonatal testis in mice and that co-PCB is potentially harmful to spermatogenesis by alteration of the development of BTB.

Attempts have been made to use Supercritical fluids for industrial purpose in a variety of fields and some of them, are already in practice. However, basic chemical properties of supercritical fluids have not been understood well. The present paper presents the results of physicochemical studies on Supercritical fluids as well as the application of supercritical fluids to industry. The detail is as follows PCB and organic compounds.

It is being more and more difficult to suppress emissions from electronic products using PCB(Printed Circuit Board) to the limit. Therefore, the exact evaluation of the emission from PCB has been more important to reduce the required time and the cost at the design phase of the products, especially on board ship's equipments. This research has evaluated the emission radiated from PCB based on the theoretical approach of SDA(Spectral Domain Analysis), which is available to analyze microwave stripline, coplanar line, patch antenna, etc. According to the theoretical results, it has been clearly shown that the emission radiated from PCB is reduced as the thickness of PCB is thinner, the permittivity of PCB is higher, the length of stripline is shorter, and the frequency is lower.