Twenty Inter simple sequence repeat (ISSR) and 30 SSR primers were used to assess genetic diversity of 64 Agaricus strains including 45 A. bisporus strains and other 19 Agaricus spp. Of them, four ISSR primers, (GA)₈T, (AG)₈YC, (GA)₈C and (CTC)₆and seven SSR markers produced PCR polymorphic bands between the Agaricus species or within A. bisporus strains. PCR polymorphic bands were inputted for UPGMA cluster analysis. Forty five strains of A. bisporus are genetically clustered into 6 groups, showing coefficient similarity from 0.75 to 0.9 among them. The varieties, Saea, saedo, Saejeong and Saeyeon that have recently been developed in Korea were involved in the same group with closely genetic relationship of coefficient similarity over 0.96, whereas, other strains were genetically related to A. bisporus strains that were introduced from USA, Eroupe and Chinese.

An 18 years old female patient suffered from cerebrovascular occlusive disease, moyamoya disease, showed a huge cyst in her left mandibular body in the radiological observation. The lesion was asymptomatic and found during routine dental check. She had no experience of traumatic injury on her jaw. The cystic lesion was ovoid with irregular scalloping margin and multilobular image, and occupied the whole marrow space of mandibular body with slight expansion of buccal cortical bone. During operation the lesion showed an empty space covered with grayish white fibrous tissue. The luminal fibrous tissue and underlying bony tissue were curettaged and examined pathologically. In the histological observation the lesion was a pseudocyst lined by thick fibrous tissue. Some large vessels underwent atherosclerotic change, exhibiting thickened vessel walls which were partly distorted with hemorrhage and thrombi, and some small capillaries were extremely dilated with hemorrhage and subsequently resulted in perivascular ischemic change with chronic vasculitis. This mandibular cystic lesion was finally diagnosed as simple bone cyst (SBC) associated with moyamoya disease differentially from aneurysmal bone cyst (ABC), traumatic bone cyst (TBC), periapical odontogenic keratocyst, and central giant cell granuloma. Therefore, it was presumed that the thromboembolic and atherosclerotic vessels of moyamoya disease might increase the hemodynamic pressure of mandibular bone marrow tissue and subsequently was able to induce SBC.

15Cr-1Mo base oxide dispersion strengthened (ODS) steel which is considered to be as a promising candidate for high- temperature components in nuclear fusion and fission systems because of its excellent high temperature strength, corrosion and radiation resistance was fabricated by using mechanical alloying, hot isostatic pressing and hot rolling. Torsion tests were performed at room temperature, leading to two different shear strain routes in the forward and reverse directions. In this study, microstructure evolution of the ODS steel during simple shearing was investigated. Fine grained microstructure and a cell structure of dislocation with low angle boundaries were characterized with shear strain in the shear deformed region by electron backscattered diffraction (EBSD). Grain refinement with shear strain resulted in an increase in hardness. After the forward-reverse torsion, the hardness value was measured to be higher than that of the forward torsion only with an identical shear strain amount, suggesting that new dislocation cell structures inside the grain were generated, thus resulting in a larger strengthening of the steel.

Chang Ucchin Museum of Art (CMA) is established by the financial support of the National Government in tribute to the memory of work and spirit of Ucchin Chang who is one of the most distinguished artists in the period of the modern Korean art history. CMA aims at exhibit, research his work and data that contributes to the following young generations for advancement of Korean modern art. Chang Ucchin Musem of Art admires the spirit of Ucchin Chang that pursuits an ideal inner world. CMA studies Korean Contemporary Art through the special exhibition by the next generation artist whose work is related to the spirit of Ucchin Chang. In addtion, CMA sets the goal for becoming a civic art museum to breathe with public and the community closely through various exhibitions, educational programs and art studio that nurtures the next generation artist. The architecture of Chang Ucchin Museum of Art is regarded as another art work that starts from the fundamental concept of house rooms where the spirit is born and grown, the space where the rooms gathers altogether like in a Korean traditional house – Hanok and – that embraces the surroundings around it. In Chang’s paintings, in general, the most appearing and very familiar objects on can-vas, such as child, bird, animal, tree and sun&moon are indeed, always with us and all harmonizes together like a natural and rural landscape living in our mind without any disturb. The art works, people, nature around the Art Museum are also the ‘neighbor’ and they create in terms of a new ‘space-relationship’ in Chang Ucchin Art Museum. The architecture designed by Chae-Pereira (Songhee Chae, Laurent Pereira) after he got inspired from imagination of Chang’s painting and won KimSwooGeun Architect Prize of 2014 (most renowned Architect Price in Korea)with it recently.

This study was carried out to study the survival rate of thawed Hanwoo embryos frozen by the slow-rate freezing or the cryotop vitrification method. Hanwoo cumulus-oocyte complexes were recovered from ovaries at a slaughter house, matured for 20~22 hours, fertilized with Hanwoo semen for 5~6 hours, and cultured for 7~9 days in 38.5℃, 5% CO2 incubator. For freezing, Day 7∼9 blastocysts were collected. Embryos for the slow-rate freezing were equilibrated in 1.8 M ethylene glycol (EG) with Dulbecco's phosphate-buffered saline (D-PBS). Programmable cell freezer was precooled down to —7℃, and the straw was seeded during 8 minutes-holding time, and was cooled to —35℃ at the cooling rate of 0.3℃/min, and then was plunged and stored in liquid nitrogen. Embryos for the cryotop vitrification were treated in TCM199 with 0.5 M sucrose, 16% EG, 16% dimethylsulfoxide (DMSO). Embryos were then loaded individually onto cryotop and plunged directly into liquid nitrogen. The survival rates of embryos frozen by these two freezing methods were evaluated at 12 to 24h post-thawing. The survival rates of frozen/thawed Hanwoo embryos by the cryotop vitrification method (56.86 ± 26.53%) were slightly higher than those by the slow-rate freezing method (55.07 ± 26.43%) with no significant difference. Using the cryotop vitrification and the slow-rate freezing of Hanwoo blastocysts on Day 7 following in-vitro fertilization (IVF) treatment, the survival rates of frozen/thawed Hanwoo embryos were 72.65 ± 18.3% and 79.06 ± 17.8%, respectively. The survival rates by the cryotop vitrification were higher than those by the slow-rate freezing on both Day 8 and 9 with significantly higher survival rate on Day 9 (p<0.05). Using the cryotop vitrification and the slow-rate freezing of Hanwoo embryos to compare between three different blastocyst stages, the survival rates of the blastocyst stage embryos were 66.22 ± 18.8% and 45.76 ± 12.8%, respectively with higher survival rate by the vitrification method (p<0.05). And the survival rate of expanded blastocysts was higher than those of early blastocysts and blastocysts in two freezing methods with significantly higher survival rate by the slow-rate freezing method (p<0.05).

The distribution of dissolved oxygen (DO) and its variability in Chunsu Bay were studied. Monthly hydrographic survey and realtime monitoring for water quality conditions were carried out in study area. Vertical distribution of DO shows the formation of hypoxia in the bottom water in the vicinity of the artificial dyke and net cage aquaculture site in summer. Annual mean of DO was 8.4 mg/l, with minimum monthly mean of 6.9 mg/l in July and maximum of 11.2 mg/l in March. Temporal variation of DO at bottom water around the net cage aquaculture site shows pronounced oscillatory characteristics with range of 0.5~7.0 mg/l. Coherency analyses between the tidal range and DO concentration shows that variation of DO is significantly coherent with tidal range at semi-diurnal period with 1~3.5 hours phase-lags. A simple DO budget model was set up to account for the formation of the extreme hypoxic conditions in Chunsu Bay. The governing equation is based on the mass-balance for kinetic process with simplified parameter of source and sink terms of DO. the source term is represented by replenishment through the vertical and horizontal diffusion and mixing. the sink terms comprise function of sediment oxygen demand (SOD) and carbonaceous biochemical oxygen demand (CBOD). The result of numerical solution indicates the dominant role of SOD as the overriding factor against the oxygen supply.

Maintaining and preserving mushroom mycelial cultures for ensuring the genetic, morphological, physiological traits and viability over time are essential elements in mushroom production. A number of different mushroom species can be preserved by many means, such as cryopreservation, freeze-drying, and placement in preservation solutions like mineral oil, distilled water, or glycerol. This study tested simple and low-cost preservation methods of the mushroom strains from various species, namely Pleurotus ostreatus, Agaricus bisporus, Cordyceps militaris. Each mycelium was cultured and preserved in different storage temperatures and methods. Temperature were differed at 4, 14, 24, 34°C at agar slants. Different storage methods were cryobial tube with sterile water, cryobial tube + 10% glycerol, agar slant of test tube, and agar slant + mineral oil at 4°C for 2 years. The mushroom cultures were better in the viability and recovery at 4°C than those of preservation at higher temperatures. Result showed that all the mushroom strains tested remained viable without any contamination under all different storage methods over two year duration of the experiment. However, all the strains studied could be best stored in sterile water with 10% glycerol for the viability.

Almost all buildings and infrastructures made of advanced composite materials are fabricated without proper design. Unlike airplanes or automobiles, prototype test is impossible. One cannot destroy 10 story buildings or 100-meter long span bridges. People try to build 100-story buildings or several thousand meter long span bridges. In order to realize "composites in construction", the following subjects must be studied in detail, for his design. Concept optimization, Simple method of analysis, Folded plate theory, Size effects in failure, and Critical natural frequency. Unlike the design procedure with conventional materials, his design should include material design, selection of manufacturing methods, and quality control methods, in addition to the fabrication method. In this paper, concept optimization and folded plate theory are presented for practicing engineers.

The purpose of this thesis is to develop a performance rating training tool for the time and motion study. It is widely recognized that rating training tools based on films were made several decades ago and introduced to Korea. However, to the best of our

가로 및 교차로에 설치된 단순입체시설의 현행 분석기법은 시설을 이용하는 주교통류를 분석대상에 포함시키지 않고 있어 불합리한 도로 및 신호운영을 유도하여 엇갈림현상, 병목현상과 같은 도로의 혼잡을 야기한다. 이러한 여건에도 불구하고 단순 입체시설을 이용하는 교통류 분석기법의 연구가 거의 수행된 바 없다. 또한 개략적으로 제시되어 있는 설정기준 역시 여러 가지 도로 및 교통조건을 반영하지 못하고 있는 실정으로, 단순입체시설이 포함된 교차로분석 시 지체도가 과소 추정되는 경향이 있다. 그러므로 본 연구는 도시내 입체시설의 효과를 파악하기 위한 선행과정으로서 대상시설의 효과를 파악할 수 있도록 입체시설의 분석기법을 개발하고 분석교차점의 적정간격을 응용하는데 그 목적이 있다. 본 연구는 다음과 같이 수행하였다. (1) 기존 분석기법의 문제점 제기, (2) 새로운 분석기법의 정립, (3) 사례지역의 적용 및 비교검정, (4) 분합류구간의 적정간격에 대한 민감도 분석을 수행한다. 연구결과, 본 연구에서 제시한 분석기법은 기존 분석기법의 모호한 부분을 명확히 하고 합리적인 결과를 추정할 수 있었다. 또한 그 분석기법을 응용하여 분합류구간에 대한 적정간격을 추정한 결과, 본선에서는 65m, 측도에서는 45m로 나타나 최소한 그 이상의 위치에 접속지점을 설치하는 것이 타당하다고 판단된다. 본 연구의 수행으로 얻어지는 가치는 다음과 같다. (1) 당해 시설에 대한 선행연구 또는 분석방법이 없었으므로 대상시설물로 인한 파급효과를 파악할 수 있다. (2) 본 연구의 분석기법은 기존 교차로의 분석방법을 차용함으로써 새로운 분석방법을 숙지하지 않아도 용이한 분석이 가능하다. (3) 본 연구에서 제시한 분석기법에 의하면 기존에 제외된 통과교통량을 포함시켜 분석할 수 있다. 이러한 방법을 통해 교차로 및 가로분석 시에 현황과 동떨어질 수 있는 결과에 대한 오류를 최소화할 수 있다. (4) 새로운 분석기법을 이용하면 신설 또는 기존 시설물에 대한 문제점을 도출하여 도로운영 및 신호운영 등의 개선방안을 제시할 수 있다.

경계요소를 가지는 철근콘크리트 전단벽의 비선형 해석을 위한 간편 모델을 제안하였다. 이 간편 모델은 전단벽의 휨 및 전단 거동을 스프링요소로 나타낸 거시적 모델이다. 휨거동은 벽체의 단면해석을 기초로 한 모멘트강도와 회전능력을 벽체 양단의 수직 스프링요소로 나타내었다. 경계요소를 가지는 전단벽은 휨거동에 의해 지배되므로 전단거동은 휨거동에 바탕하여 변수를 계산하였고 중앙부 수평 스프링요소로 나타내었다. 제안된 모델은 전단벽 정적이력시험 결과와 비교한 후 비선형동적해석을 수행하여 사용된 이력법칙 및 변수들의 타당성을 조사하였다. 비선형동적해석을 이용한 변수연구를 통하여 내진성능평가의 주요변수인 요구값과 성능값에 미치는 영향을 검토하였다. 그 결과 전단력-전단변형 관계에서 약간의 차이가 있지만 전단벽의 전체거동은 잘 일치하였으며, 주요 변수의 변화에 대해 요구값과 성능값도 일정하게 변화하므로 제안된 해석모델은 경계요소를 가진 철근콘크리트 전단벽에 알맞은 것으로 판단된다.

Seven male Himalayan tahrs were strongly assumed to have accidently fed on foreign bodies in 2007. At the time, zoo caretakers witnessed missing fence padding, such as carpet, plastic awning, and ropes. The incident occurred the morning after the items had been set up to protect indigenous, wild long-tailed gorals from self injury caused by head butting the steel fence. Adult male Himalayan tahrs were obviously suspected of mostly eating the paddings mainly composed of carpet, thin and long plastic awning, and ropes. Even though they had not shown digestive problems, surgery was determined necessary in order to remove any indigestible foreign bodies. Left flank rumenotomy was conducted on seven male Himalayan tahrs from April to May, 2011. After anesthesia with xylazine, rumenotomy was performed on a concrete floor, with legs and head secured by ropes. No access to water and hay prior to operation for two days was needed to make the surgical procedure done quickly. Two sheets of small hand towels protecting against inflow of ruminal contents were beneficial during surgery. Antibiotics were administered intramuscularly for seven days. No abscesses at the surgical site were found after surgery. Like domestic ruminants, wild ruminants also ingest metalic or non-metalic, indigestible foreign bodies by accident. Therefore, simplified rumenotomy must be developed to apply to those wild animals to lengthen their longevity and to advance the quality of life in captivity. This case report is the first showing how to perform rumenotomy of Himalayan tahr, a wild ruminant, in Korea.

In this paper, two dimensional concrete slabs for a railroad bridge were analyzed by the specially orthotropic laminates theory. Both the geometrical and material property of the cross section of the slab was considered symmetrically with respect to the neutral surface so that the bending extension coupling stiffness, Bij=0, and D16=D26=0. Bridge deck behaves as specially orthotropic plates. In general, the analytical solution for such complex systems is very difficult to obtain. Thus, finite difference method was used for analysis of the problem. In this paper, the finite difference method and the beam theory were used for analysis.

대부분의 건설기술자들은 강판형교나 콘크리트 교량을 단위 폭을 가진 보로 해석 하나 이러한 구조물은 엄연히 복합재료로 이루어진 판(plate) 구조이다. 이러한 구조물은 균등단면에 등분포하중을 받고 4변 단순 지지된 경우가 아니면 정확한 해석이 불가능하다. 복합 재료의 이론은 일반 기술자들이 이해하기 힘드나. 본 논문에서는 이러한 문제를 비교적 쉽게 정확히 해석하는 방법을 제시한다. 본 논문에서는 3경간 연속 판 교량과 포스트텐션된 강교에 대한 수치해석을 수행하였다.

This study investigated whether the simple view of reading framework is supported among Upper elementary Korean EFL learners. Specifically, the relative contributive power of two emergent literacy factors, word decoding and linguistic comprehension abilities, which have been identified as the main determinants of successful reading comprehension, was examined. Ninety nine fifth grade students in Korean elementary school participated in this research, and their decoding skills, listening comprehension abilities, and reading comprehension in English were measured. The findings revealed that both English decoding skills and linguistic comprehension ability were significant predictors of their English reading comprehension, which supports the simple view of reading within the Korean EFL context. Specifically, decoding skill explained more of the variance, compared to linguistic comprehension, in reading comprehension when controlling for each other. The result is discussed in terms of the overall development of L2 proficiency and the role of L2 exposure in L2 reading comprehension development.