Powder library of pseudo four components Li-Ni-Co-Ti compounds were prepared for exploring the composition region with the single phase of the layer-type structure by using combinatorial high-throuput preparation system "M-ist Combi" based on electrostatic spray deposition method. The new layer-type compounds were found wider composition region than the previous report. This process is promising way to find multi component functional materials.

이식 전 수정란의 성판정은 많은 축산인의 소망이었다. 많은 방법이 제기되었지만, 세포유전학적 분석방법은 ET의 상용화에 거의 무의미할 정도로 한계가 많았고, 이후 개발된 응성 특이적 항원이나 X-염색체에서 발현되는 효소의 활동을 통한 성판정 방법은 흥미롭기는 하나 산업적 적용에 제약이 많았다. 80년대 중반 Y-염색체 특이적인 DNA 탐침자의 개발과 PCR을 통한 DNA증폭기술의 개발은 수정란 성판정을 획기적으로 개선시켰다. DNA기술을 통한 수정란의

This study investigates the concentration distribution of aldehydes and volatile organic compounds (VOCs) in the archive of the National Library in Korea and evaluates the health risks to workers from hazardous chemicals. Acetaldehyde had the highest concentration among the nine species of aldehydes present in the archive and the concentration of toluene was the highest among the six species of VOCs. Most of the detected substances showed that their indoor concentrations were higher than the outdoor ones, suggesting the possibility of indoor sources of aldehydes and VOCs. The evaluation of health risks for workers based on these measurement results showed that not all substances were hazardous to the human body. However, considering the possibility of the presence of indoor sources and the potential limits of our study owing its short period, it is necessary to conduct long-term studies on the concentration distribution of indoor pollutants in the archive environment.

본 논문은 연세대 학술정보원에 소장되어 있는 『여유당집(與猶堂集)』을 대상으로 서지적인 측면에서 몇 가지 사항을 검토하여 본 것이다. 연세대본 『여유당집』은 1책으로 된 필사본으로 다산 정약용(丁若鏞 )의 시문(詩文)을 싣고 있는데 이 책 안에 다산의 형인 정약전(丁若銓)의 시문이 함께 수록되어 있다. 연세대본 􋺷여유당집􋺸은 형태적인 특징이 다산 자신이 만년(晩年)에 정리하였다고 하는 가장(家藏) 고본(藁本)의 체재와는 다르며 다산과 제자들 사이에서 생산된 친필본(親筆本) 계통에도 속하지 않는다. 필사기와 수록 작품의 내용을 보아 편집하고 필사한 년도는 1812년으로 보인다. 이 책의 구성은 <여유당집(與猶堂集)>, <여유당시집(與猶堂詩集)>, ‘의(議)’ 작품, 관문서(官文書)의 순으로 실려 있다. <여유당집>에는 중형인 정약전에게 보낸 편지 9편과 「잡설(雜說)」 1편으로 되어 있고 「잡설」에 대한 정약전의 글이 부록으로 들어가 있다. <여유당시집>에는 다산의 작품과 함께 정약전의 시 작품이 함께 수록되어 있다. ‘의’ 작품들도 정약전의 「송정사의(松政私議)」와 함께 다산의 ‘의’ 3편이 나란히 수록되어 있다. 관문서 3편은 모두 정약전이 유배 생활을 하던 흑산도(黑山島)와 관련된 문서들이다. 본문 내용을 『여유당전서』와 대조하여 보면 연세대본 『여유당집』의 내용 중 상당 부분의 어구들이 축약되어 『여유당전서(與猶堂全書)』에 수록되어 있고, 문자 상의 차이도 많이 나타나고 있다. 반면 『여유당전서』에 나오는 어구들이 이 연세대본에는 생략되어 있는 경우도 있어 다산 저술의 전승과정에 또 다른 이본(異本)이 존재하여 『여유당전서』의 편찬 과정에 수용되었다는 것을 추정할 수 있다. 이 연세대본 『여유당집』은 정약용과 정약전의 유배 시기에 이 두 사람과 밀접한 관계가 있던 인물에 의해 편찬된 것으로 보인다. 이 책은, 정약전의 시문이 함께 수록되어 있다는 점 외에도, 정약용의 작품이 후대에 간행된 『여유당전서』와 본문 내용 등에서 많은 차이를 보이고 있어 다산의 생애나 작품의 연구, 다산 저술의 전승과정을 규명하는 데에도 도움이 될 것이다.

지금까지 양구슬냉이의 유전정보는 거의 연구되지 않았으므로 우리는 양구슬냉이의 잎으로부터 cDNA library를 제작하고 발현유전자의 종류와 기능별 분류를 조사하였다. 그 결과를 요약하면 다음과 같다. 1. cDNA library에서 1334개 의 클론들을 얻었고 삽입된 단편들의 염기서열의 평균길이는 736bp였다. 우리는 1269개의 high-quality expressed sequence tags (ESTs) 서열을 얻었다. 이러한 EST의 클러스터 분석결과 고유 염기서열(unigene)을 가진 유전자의 수는 851개를 나타냈다. 2. Unigene 476개는 GeneBank에 기능이 알려진 유전자들과 고도의 상동성을 나타내었다. 다른 375개의 unigene들은 기능이 알려지지 않은 것들이었다. 나머지 63개는 NCBI데이터베이스에 어떤 유전자와도 상동성을 보이지 않았고 이러한 유전자들은 아마도 양구슬냉이의 잎에서 발현되는 새로운 유전자일 것으로 보인다. 3. 데이터베이스에서 상동성을 나타낸 EST들을 기능별 주석에 따라서 17개의 카테고리로 분류하였다. 대표적으로 가장 분포도가 높은 카테고리는 결합 기능 또는 보조인자 요구의 단백질(27%), 대사(11%), 세포 소기관 위치(11%), 세포수송과 수송기관 그리고 수송 경로(7%), 에너지(6%), 대사와 단백질 기능의 조절(6%) 등이 있다. 이러한 우리의 연구 결과는 양구슬냉이의 유용한 유전적 자원과 전반적인 mRNA 발현 정보를 제공해 줌으로써 대체 에너지 작물로 떠오르는 양구슬냉이의 다양한 분자적 연구에 기여할 것으로 사료된다.

To develop the STS (Sequence Tagged Site) marker for discrimination between oat cultivars used the EEG (Euchromatin Enriched Genomic) DNA library in oat. The EEG DNA library was constructed the Mcr A and Mcr BC system in DH5 alpha bacterial cell line. About 3,500 EEG colonies had been constructed by using junk DNA exclusion. About 800 colonies were selected that included insert DNA more than 500 bp. It was analyzed the genetic information by using blast searches of NCBI web site. More than three hundred STS primer sets were developed using sequencing data of selected colonies and about 90 primer sets which showed single band were selected in Olgwiri. It was applied to twelve oat cultivars including Olgwiri and has been shown polymorphism at 15%. PCR product which amplified with selected STS primer was treated with six endonucleases and was showed polymorphic bands. These primers could be useful for specific allele tagging in mapping populations and germplasm and for the study of functional genomics of oat.

Camelina sativa (L.) Crantz, known as popular names "gold-of-pleasure" or "false flax" is an alternative oilseed crop that can be grown under different climatic and soil condition. Up to date, however, the genomic information of camelina has not been studied in detail. Therefore, a cDNA library was constructed and characterized from young leaves. The constructed cDNA library incorporated of 1334 cDNA clones and the size of the insertion fragments average was 736 base pair. We generated a total of 1269 high-quality expressed sequence tags (ESTs) sequences. The result of cluster analysis of EST sequences showed that the number of unigene was 851. According to subsequent analysis, the 476 unigenes were highly homologous to known function genes and the other 375 unigenes were unknown. Remaining 63 unigenes had no homology with any other peptide in NCBI database, indicating that these seemed to be novel genes expressed in leaves of camelina. The database-matched ESTs were further classified into 17 categories according to their functional annotation. The most abundant of categories were protein with binding function (27%), metabolism (11%), subcellular localization (11%), cellular transport, transport facilities and transport routes (7%), energy (6%), regulation of metabolism and protein function (6%). Our result in this study provides an overview of mRNA expression profile and a basal genetic information of camelina as an oilseed crop.

Perilla is a genus as a member of the mint family Lamiaceae which is known to contain lots of volatile metabolite. Perilla has been called as ‘deulkae’ indicating ‘wild sesame’ that means it has been maintained in Korea with long history. It has been very friendly used as edible oil and as fresh leaf vegetable. Perilla oil is valued for its medicinal benefit because it contains best amounts of unsaturated fatty acids, especially for the alpha-linolenic acid, known to omega-3 fatty acid, among all of the plant oils. It also include many beneficial phytochemicals. However, little study is conducted on their genetics. Here, we announce construction of well normalized and full length enriched-perilla cDNA library from a whole plant of one cultivar ‘Youngho-deulkae’ and their sequence characterization to provide useful resources for genetics, breeding and metabolite engineering. By sequencing of 5,760 cDNA clones, we 5,438 high quality EST sequences. Sequence trimming and assembly resulted 3,995 unigenes which consists 1,004 contigs and 2,991 singletones. Unigenes that showed little homology at the DNA sequence level with known genes in other plants even though they showed similarity at the protein domain level based on BLASTN, BLASTX, and TBLASTX. This study may provide good resources for initiation of further genomics, comparative genomics, functional genomics such as metabolic engineering and molecular breeding.

For the development of SSR marker system in Vicia villosa Roth, an enriched library was constructed by using a modified biotin-streptavidin capture method and the selected clones were sequenced with GS-FLX(454). Of 37,794 sequenced reads, we found that 8,474 reads (22.4%) were redundant, leaving 29,320 unique ones (77.6%). Among the unique clones, 17,174 reads (58.6%) were having microsatellite repeating motifs. Sequence analysis of all SSR-containing reads revealed a predominance of the di-nucleotide SSRs (62.5%). The tri-nucleotide and the tetra-nucleotide SSRs were 5.7% and 22.5%, respectively. As the di-nucleotide type, the AG/GA class of repeat motif was most frequently identified (55.0% of the total di-nucleotide SSRs), followed by the CT/TC class (19.5%), and the TA/AT class (12.1%). Among the tri-nucleotide SSRs, the AGT/GTA/TAG class of repeat motifs was predominant (22.2%), followed by the ACT/CTA/TAC class (17.8%). Among the tetra-nucleotide SSRs, the CTTT/TTTC/TTCT/TCTT class of repeat motifs was predominant (31.2%), followed by the AAAG/AAGA/AGAA/ GAAA class (19.9%). Finally, we designed 779 primer pairs from the flanking sequences of SSR containing reads. We are undertaking the analysis of polymorphisms using the diverse collected accessions of Vicia villosa Roth now. This newly developed SSR marker set shall provide a very useful tool for implementing molecular diversity assessment and population structure studies of Vicia villosa Roth onward.

The purpose of this study is to develop the EEG (Euchromatin Enriched Genomic) DNA library of wheat, barley, rye and oat. Mcr A and Mcr BC system in DH5 alpha bacteria cell line and Kuemkangmil, Olbori, Olhomil and Olgwiri were used for materials in our experiments. EEG colonies have been constructed by using junk DNA exclusion. We analyzed the genetic information of the colonies using blast searches of NCBI and GRAMENE web sites. One hundred eighty-four, 65, 79 and 119 STS primer pairs were developed using sequencing data of selected colonies in Kuemkangmil, Olbori, Olhomil and Olgwiri respectively. Twenty-eight and forty-two percent of designed primer pair showed polymorphism using six endoucleases in Kuemkangmil, Olbori, Olhomil and Olgwiri germplasm respectively. These primers could be useful for specific allele tagging in mapping populations and germplasm and for the study of functional genomics of wheat, barley, rye and oat.