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        검색결과 374

        102.
        2016.04 구독 인증기관·개인회원 무료
        Phosphine(PH3) is very effective fumigant and is widely used to control pests. Oxygen treatment was found to enhance phosphine toxicity and reduce fumigation time. We researched the insecticidal activities and synergistic effects of oxygen with phosphine(PH3) against on adult stage of 6 agricultural pests as Frankliniella occidentalis, F. intonsa, Pseudococcus comstocki, Planococcus citri, Myzus persicae and Tetranychus urticae. Adults F. occidentalis, F. intonsa, P. comstocki and P. citri was highly susceptible to PH3 at 2mg/L for 2h treatment. However, M. persicae and T. urticae were showed very low fumigation effects at 2mg/L for 2h treatment. These 2 pests were treated with PH3 to increase the times (16h and 20h). And we also researched the synergistic effects of PH3 under controlled atmospheres of 50% and 80% oxygen(O2). Increase of the time and atmospheric oxygen showed improving activities of the PH3 in the 2 agricultural pests. Therefore, PH3 is an effective fumigant against F. occidentalis, F. intonsa, P. comstocki and P. citri, and its synergistic effect with oxygen is effective to control M. persicae and T. urticae. PH3 with oxygen could be useful for managing the agricultural and quarantine pests.
        103.
        2016.03 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Nitrogen (N)-doped ordered mesoporous carbons (OMCs) with a dual transition metal system were synthesized as non-Pt catalysts for the ORR. The highly nitrogen doped OMCs were prepared by the precursor of ionic liquid (3-methyl-1-butylpyridine dicyanamide) for N/C species and a mesoporous silica template for the physical structure. Mostly, N-doped carbons are promoted by a single transition metal to improve catalytic activity for ORR in PEMFCs. In this study, our N-doped mesoporous carbons were promoted by the dual transition metals of iron and cobalt (Fe, Co), which were incorporated into the N-doped carbons lattice by subsequently heat treatments. All the prepared carbons were characterized by via transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoelectron spectroscopy (XPS). To evaluate the activities of synthesized doped carbons, linear sweep was recorded in an acidic solution to compare the ORR catalytic activities values for the use in the PEMFC system. The dual transition metal promotion improved the ORR activity compared with the single transition metal promotion, due to the increase in the quaternary nitrogen species from the structural change by the dual metals. The effect of different ratio of the dual metals into the N doped carbon were examined to evaluate the activities of the oxygen reduction reaction.
        4,300원
        104.
        2016.03 구독 인증기관 무료, 개인회원 유료
        Chronic/cyclic neutropenia, leukocyte adhesion deficiency syndrome, Papillon-Lefèvre syndrome, and Chédiak-Higashi syndrome are associated with severe periodontitis, suggesting the importance of neutrophils in the maintenance of periodontal health. Various Toll-like receptor (TLR) ligands are known to stimulate neutrophil function, including FcR-mediated phagocytosis. In the present study, the effect of TLR2 activation on the non-opsonic phagocytosis of oral bacteria and concomitant production of reactive oxygen species (ROS) by human neutrophils was evaluated. Neutrophils isolated from peripheral blood were incubated with Streptococcus sanguinis or Porphyromonas gingivalis in the presence of various concentrations of Pam3CSK4, a synthetic TLR2 ligand, and analyzed for phagocytosis and ROS production by flow cytometry and chemiluminescence, respectively. Pam3CSK4 significantly increased the phagocytosis of both bacterial species in a dose-dependent manner. However, the enhancing effect was greater for S. sanguinis than for P. gingivalis. Pam3CSK4 alone induced ROS production in neutrophils and also increased concomitant ROS production induced by bacteria. Interestingly, incubation with P. gingivalis and Pam3CSK4 decreased the amounts of ROS, as compared to Pam3CSK4 alone, indicating the possibility that P. gingivalis survives within neutrophils. However, neutrophils efficiently killed phagocytosed bacteria of both species despite the absence of Pam3CSK4. Although P. gingivalis is poorly phagocytosed even by the TLR2-activated neutrophils, TLR2 activation of neutrophils may help to reduce the colonization of P. gingivalis by efficiently eliminating S. sanguinis , an early colonizer, in subgingival biofilm.
        4,000원
        105.
        2015.11 구독 인증기관·개인회원 무료
        산소 분리를 위한 세라믹 중공사막을 상전이 방적기술을 통해 제조하였다. 초기 BSCF 선구물질은 고상반응법을 이용하여 합성한 상용 분말이며, 고분자 용액에 분산시킨 후 이중관형 노즐을 통해 사출하였다. 사출된 분리막은 상전이 과정을 거친 후 건조시켰으며 중공사막의 한쪽 끝을 밀봉하였다. 한쪽 끝이 막힌 중공사막의 표면에 dip coating 방법으로 LSCF를 코팅하였으며 1100 ℃에서 소결하여 치밀성을 갖는 한쪽 끝이 막힌 LSCF coated BSCF 중공사막을 제조하였다. 분리막의 공급측은 대기 중 공기를 사용하였으며 투과측은 진공상태를 유지하였다. 투과된 기체의 유량 및 산소의 농도를 측정하였으며 장기투과 실험과 EDS 분석을 통해 분리막의 안정성 평가를 진행하였다.
        108.
        2015.10 KCI 등재 구독 인증기관 무료, 개인회원 유료
        고상반응법을 이용하여 SrCo0.8Fe0.1Nb0.1O3-δ 조성의 산화물을 합성하였으며, 합성된 분말은 압축 성형 후 1250°C 에서 소결하여 치밀한 세라믹 분리막을 제조하였다. XRD 분석을 통해 단일상의 페롭스카이트 구조를 확인하였다. 산소 분압 이 0.21 atm, 측정 온도가 800~950°C인 조건하에서 산소투과를 분석한 결과 온도가 증가할수록 산소투과량은 증가하였고, 950°C에서 1.839 mL/min⋅cm2로 최대값을 나타내었다. 니오븀(Nb)을 포함한 세라믹 분리막의 상안정성 및 이산화탄소 내성을 확인하기 위하여 900°C에서 이산화탄소가 500 ppm이 포함된 혼합공기를 이용하여 장기투과 실험을 수행하였다. 이산화탄소에 노출된 SrCo0.8Fe0.1Nb0.1O3-δ의 상안정성은 XRD와 TG로 분석하였다. 분석 결과, 이산화탄소에 노출된 SrCo0.8Fe0.1Nb0.1O3-δ 조 성의 경우 약 8%의 SrCO3가 생성되었으나, 이 수준의 SrCO3 생성량은 분리막의 산소투과도에 큰 영향을 주지 않는 것을 확 인하였다.
        4,000원
        109.
        2015.10 구독 인증기관·개인회원 무료
        Many forest pests present a problem during wood quarantine. The effectiveness of phosphine (PH3), which is an altered form of MB, was examined in Monochamus saltuaris and M. alternatus. After 24 h, the larvae and the adults of both insects were susceptible to PH3 at 3 mg/L at 20°C. However, the larvae of both pests were rather than dead, seem to have been quiescent condition. PH3 showed increasing insecticidal activity in a time-dependent manner in the larva stage of both pests. We investigated the fumigants at all stages of M. saltuaris and M. alternatus, as well as the synergistic effects of PH3 in controlled atmospheres of 50% and 80% oxygen. The atmospheric oxidation of PH3 fumigation slightly increased the toxicity of the fumigant to larva stages in 125 L container at 4 mg/L. These results indicate that highly concentrated atmospheric oxidation of PH3 could be useful as a fumigant agent against M. saltuaris and M. alternatus.
        110.
        2015.08 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This paper is concerned with the optimal design of hyperbaric oxygen therapy system chamber with separated rooms for multi-users using stress analysis. Hyperbaric oxygen therapy is very important medical remedy for increasing the concentration of melted oxygen in human and animal body to recover the impaired cell for the healthy condition. This research proposed the proper procedures to design the physical specifications and investigate the mechanical design safety of hyperbaric oxygen chamber with two separated rooms using computational stress analysis with Solidworks program. The result of this research will be used to make the real size model of practical hyperbaric oxygen therapy system for multi-users with separated chamber.
        4,000원
        111.
        2015.06 구독 인증기관 무료, 개인회원 유료
        Growing evidence suggests that mitochondrial reactive oxygen species (ROS) are involved in various pain states. This study was performed to investigate whether ROS-induced changes in neuronal excitability in trigeminal subnucleus caudalis are related to ROS generation in mitochondria. Confocal scanning laser microscopy was used to measure ROS-induced fluorescence intensity in live rat trigeminal caudalis slices. The ROS level increased during the perfusion of malate, a mitochondrial substrate, after loading of 2′,7′-dichlorofluorescin diacetate (H2DCF-DA), an indicator of the intracellular ROS; the ROS level recovered to the control condition after washout. When pre-treated with phenyl N-tert-butylnitrone (PBN) and 4-hydroxy-2,2,6,6-tetramethylpiperidene-1-oxyl (TEMPOL), malate-induced increase of ROS level was suppressed. To identify the direct relation between elevated ROS levels and mitochondria, we applied the malate after double-loading of H2DCF-DA and chloromethyl-X-rosamine (CMXRos; MitoTracker Red), which is a mitochondria- specific fluorescent probe. As a result, increase of both intracellular ROS and mitochondrial ROS were observed simultaneously. This study demonstrated that elevated ROS in trigeminal subnucleus caudalis neuron can be induced through mitochondrial-ROS pathway, primarily by the leakage of ROS from the mitochondrial electron transport chain.
        4,000원
        112.
        2015.05 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Abalone (Haliotis discus hannai) is a shellfish that feeds on kelp and, as a product, it can often achieve a high market value. However, the dissolved oxygen (DO) levels in coastal waters in Korea have been negatively impacted by pollution from many anthropogenic sources. Herein, a computational fluid dynamics (CFD) software package was used to analyze the distribution of the DO concentration within an abalone containment structure. A finite volume approach was used to solve the Reynolds-averaged Navier–Stokes equations combined with a k–ε turbulence model to describe the flow. The distribution of DO was determined within the control volume domain, and the transport equations of the pollutants were interpreted using a CFD model. The CFD analysis revealed that more than 60% and 30% of the relative oxygen concentration in one and two containers, respectively, was maintained when the flow acts along the six sheets of polyethylene plates. Therefore, it is clear that the abalone plate shelters should be placed parallel to the flow.
        4,000원
        113.
        2015.05 구독 인증기관·개인회원 무료
        산소 분리를 위한 세라믹 중공사막을 상전이 방적기술을 통해 제조하였다. Ba0.5Sr0.5Co0.8Fe0.2O3-δ 선구 물질을 고분자 용액에 분산시킨 후 이중관형 노즐을 통해 사출한 후 상전이, 건조한 후 분리막의 한쪽 끝을 밀봉하였다. La0.6Sr0.4Ti0.3Fe0.7O3-δ 코팅 층은 dip coating 방법으로 제조되었으며 최종적으로 고온에서 소결하여 La0.6Sr0.4Ti0.3Fe0.7O3-δ로 코팅된 one end-closed type Ba0.5Sr0.5Co0.8Fe0.2O3-δ 중공사막을 제조하였다. 산소투과실험은 대기 중 공기를 사용하였으며 진공펌프를 연결하여 투과된 산소 유량 및 순도를 측정하였다.
        114.
        2015.04 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The monolayer engineering diamond particles are aligned on the oxygen free Cu plates with electroless Ni plating layer. The mean diamond particle sizes of 15, 23 and 50 μm are used as thermal conductivity pathway for fabricating metal/carbon multi-layer composite material systems. Interconnected void structure of irregular shaped diamond particles allow dense electroless Ni plating layer on Cu plate and fixing them with 37-43% Ni thickness of their mean diameter. The thermal conductivity decrease with increasing measurement temperature up to 150oC in all diamond size conditions. When the diamond particle size is increased from 15 μm to 50 μm (Max. 304 W/mK at room temperature) tended to increase thermal conductivity, because the volume fraction of diamond is increased inside plating layer.
        3,000원
        115.
        2015.02 구독 인증기관 무료, 개인회원 유료
        Luteolysis is a cyclical regression of the corpus luteum in many non-primate mammalian species. Prostaglandin F2α (PGF2α) from the uterus and ovary induces functional and structural luteolysis in bovine. The action of PGF2α is mediated by PGF2α receptor located on the luteal steroidogenic and endothelial cell membranes. PGF2α plays an important role in regulating nitric oxide production in endothelial cells of the bovine corpus luteum. Nitric oxide production and nitric oxide synthase activity are stimulated and induced by PGF2α in luteal endothelial cells. Moreover, the reactive oxygen species inhibits progesterone secretion in bovine luteal cells and induces apoptosis. Thus, the interaction between PGF2α and reactive oxygen species provides important aspects in physiology of the corpus luteum forfunctional and structural luteolysis.
        4,000원
        116.
        2015.02 KCI 등재 구독 인증기관 무료, 개인회원 유료
        This paper is concerned with the mechanical design of hyperbaric oxygen chamber for multi-users using stress analysis. HBOT(Hyperbaric Oxygen Therapy) is very effective medical equipment for increasing the concentration of melted oxygen in human and animal body. The hyperbaric oxygen supplies to the impaired cell of human and animal to recover the healthy condition. This research reported the design specifications and mechanical safety of hyperbaric oxygen chamber using computational stress analysis with CATIA program. The result from this research can be used for making the practical HBOT equipment for multi-users and to manufacture the real model
        4,000원
        117.
        2015.01 KCI 등재 구독 인증기관 무료, 개인회원 유료
        The breakthrough behaviour of activated charcoal cloth samples against an oxygen analogue (OA) of sulphur mustard has been studied using the modified Wheeler equation. Activated charcoal cloth samples having different surface area values in the range of 481 to 1290 m2/g were used for this purpose. Breakthrough behaviour was found to depend on the properties of the activated charcoal cloth, properties of the OA and the adsorption conditions. Activated charcoal cloth with a high surface area of 1290 m2/g, relatively large surface density of 160 g/m2 and coarser fiber structure exhibited better kinetic saturation capacity value, 0.19 g/g, against OA vapours when compared to others, thus confirming its potential use in foldable masks for protection against chemical warfare agents.
        4,000원
        118.
        2014.12 구독 인증기관 무료, 개인회원 유료
        Reactive oxygen species (ROS) and nitrogen species (RNS) are implicated in cellular signaling processes and as a cause of oxidative stress. Recent studies indicate that ROS and RNS are important signaling molecules involved in nociceptive transmission. Xanthine oxidase (XO) system is a well-known system for superoxide anions (O2˙-) generation, and sodium nitroprusside (SNP) is a representative nitric oxide (NO) donor. Patch clamp recording in spinal slices was used to investigate the role of O2˙- and NO on substantia gelatinosa (SG) neuronal excitability. Application of xanthine and xanthine oxidase (X/XO) compound induced membrane depolarization. Low concentration SNP (10μM) induced depolarization of the membrane, whereas high concentration SNP (1 mM) evoked membrane hyperpolarization. These responses were significantly decreased by pretreatment with phenyl N-tert-butylnitrone (PBN; nonspecific ROS and RNS scavenger). Addition of thapsigargin to an external calcium free solution for blocking synaptic transmission, led to significantly decreased X/XO-induced responses. Additionally, X/XO and SNP-induced responses were unchanged in the presence of intracellular applied PBN, indicative of the involvement of presynaptic action. Inclusion of GDP-β-S or suramin (G protein inhibitors) in the patch pipette decreased SNP-induced responses, whereas it failed to decrease X/XO-induced responses. Pretreatment with n-ethylmaleimide (NEM; thiol-alkylating agent) decreased the effects of SNP, suggesting that these responses were mediated by direct oxidation of channel protein, whereas X/XO-induced responses were unchanged. These data suggested that ROS and RNS play distinct roles in the regulation of the membrane excitability of SG neurons related to the pain transmission.
        4,000원
        119.
        2014.12 구독 인증기관 무료, 개인회원 유료
        Antimicrobial actions of reactive oxygen/nitrogen species (ROS/RNS) derived from products of NADPH oxidase and inducible nitric oxide (NO) synthase in host phagocytes inactivate various bacterial macromolecules. To cope with these cytotoxic radicals, pathogenic bacteria have evolved to conserve systems necessary for detoxifying ROS/RNS and repairing damages caused by their actions. In response to these stresses, bacteria also induce expression of molecular chaperones to aid in ameliorating protein misfolding. In this study, we explored the function of a newly identified chaperone Spy, that is localized exclusively in the periplasm when bacteria exposed to conditions causing spheroplast formation, in the resistance of Salmonella Typhimurium to ROS/RNS. A spy deletion mutant was constructed in S. Typhimurium by a PCR-mediated method of one-step gene inactivation with λ Red recombinase, and subjected to ROS/RNS stresses. The spy mutant Salmonella showed a modest decrease in growth rate in NO-producing cultures, and no detectable difference of growth rate in H2O2 containing cultures, compared with that of wild type Salmonella. Quantitative RT-PCR analysis showed that spy mRNA levels were similar regardless of both stresses, but were increased considerably in Salmonella mutants lacking the flavohemoglobin Hmp, which are incapable of NO detoxification, and lacking an alternative sigma factor RpoS, conferring hypersusceptibility to H2O2. Results demonstrate that Spy expression can be induced under extreme conditions of both stresses, and suggest that the protein may have supportive roles in maintaining proteostasis in the periplasm where various chaperones may act in concert with Spy, thereby protecting bacteria against toxicities of ROS/RNS.
        4,000원
        120.
        2014.12 KCI 등재 구독 인증기관 무료, 개인회원 유료
        Effect of oxygen content in the ultrafine tungsten powder fabricated by electrical explosion of wire method on the behvior of spark plasma sintering was investigated. The initial oxygen content of 6.5 wt% of as-fabricated tungsten powder was reduced to 2.3 and 0.7 wt% for the powders which were reduction-treated at 400˚C for 2 hour and at 500˚C for 1h in hydrogen atmosphere, respectively. The reduction-treated tungsten powders were spark-plasma sintered at 1200-1600˚C for 100-3600 sec. with applied pressure of 50 MPa under vacuum of 0.133 Pa. Maximun sindered density of 97% relative density was obtained under the condition of 1600˚C for 1h from the tungsten powder with 0.7 wt% oxygen. Sintering activation energy of 95.85kJ/mol-1 was obtained, which is remarkably smaller than the reported ones of 380~460kJ/mol-1 for pressureless sintering of micron-scale tungsten powders.
        4,000원