본 연구에서는 고량(Sorghum nervosum) 70 % 에탄올 추출 후 항염증 및 UVB에 대한 세포 보호 효과를 검증하고자 하였다. 고량 추출물의 효능 평가는 세포생존율분석, 활성산소 측정, 항염증, COX-2 단백질 변화, UVB에 대한 세포 보호 효과를 수행하였다. 실험 결과, RAW264.7 대식세포, HaCaT 세포에서 고량추출물 모든 농도에서 97 %이상 세포 생존율을 확인하였다. 항염증 NO 생성 억 제능에서는 농도 의존적으로 저해 효과가 나타났으며, COX-2 단백질 발현량 역시 25, 50 ㎍/mL 농도 에서 유의하게(p＜.001) 저해되었다. UVB에 대한 세포 보호 효과로는 세포 내 활성산소종(ROS) 정량 분석 결과, 고량 추출물이 ROS 총 양 감소에 효과가 있음을 확인하였다. 연구결과를 종합적으로 고려해 볼 때, 고량추출물은 항염증 및 UVB에 대한 세포 보호 기능을 나타내는 화장품 원료로서의 개발 가능 성이 있다고 사료된다.

The purpose of this investigation was to evaluate anti-tumor activity and detect what compounds affect its activity form Alpinia officinarum Hance. Two fractions, methanol and ethylacetate, were isolated by Amberlite XAD-2 resin column chromatography from methanol extract of the rhizomes of Alpinia officinarum. In hollow fiber assay, the methanol extract and methanol fraction were found to inhibit the tumor growth against colon tumor cell lines such as Colo-320, HCT116 and WiDr. Three diarylheptanoids [5-hydroxy-1, 7-diphenyl-3-heptanone, 5-hydroxy-7-(4``-hydroxy-3``-methoxyphenyl)-1-phenyl- 3-heptanone and 3, 5-dihydroxy-1, 7-diphenylheptane] and two flavonoids [galangin and kaempheride] were isolated and identified from the methanol fraction, which is higher activity than ethylacetate fraction. Among these diarylheptanoids and flavonoids, 3, 5-dihydroxy-1, 7-diphenylheptane, galangin and kaempheride as active components on anti-tumor activity were mainly posited in methanol fraction.

Background : Alpinia Officinarum Rhizome (高良薑) is recorded as a roots of Alpinia officinarum Hance (Zingiberaceae) in Korea Pharmacopoeia and there is no established confirmation test and method for quantitative determination using marker compound. Using the six marker compounds, pinocembrin, (5S)-5-hydroxy-7- (4-hydroxy-3-methoxyphenyl) -1-phenyl-3-heptanone, pinocembrin, galangin, kaemferide, galangin 3-methyl ether, A simultaneous analysis method was developed to utilize the quality control. Methods and Results : In this study, YMC-pack column (ODS C18, 250 × 4.6 ㎜, 5 ㎛) from YMC was used and the temperature was set at 30℃. A soultion of 0.1% formic acid was using water (mobile phase A) and acetonitrile (mobile B), respectively, and the mobile phase B was set to 15 - 35% for 0 - 15 minutes and 35 - 40% for 15 - 60 minutes. And 270 ㎚ was set as the detection wavelength. The order of the components is pinocembrin (1, Rt: 23.2 min), (5S)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (2, Rt: 37.8 min), pinocembrin (3, Rt: 44.9 min), galangin (4, Rt: 46.7 min), kaemferide (5, Rt: 48.6 min), galangin 3-methyl ether (6, Rt: 52.8 min) and Based on the retention time, ultraviolet spectrum and MS value, it was confirmed by comparison with standard compounds. Conclusion : In this study, we developed the simultaneous analysis method by six marker components of the Alpina officinarum Hance extract to develop a quality test method to scientifically identify Alpinia officinarum Hance.

치자로부터 청색 및 황색색소, 고량으로부터 암갈색 색소를 주정을 이용하여 추출ㆍ농축하여 60Brix 농도의 추출물을 회수하였는데, 각각의 수율은 0.68, 1.97, 0.63% (w/w)였다. 이들을 대두유: 물: 유화제: 치자청: 치자황: 고량색소=8: 22: 42: 10: 15: 13 (w/w)의 비율로 혼합하고 균질화하여 유용성 천연 검정색소를 얻었다. 이 색소를 대두유에 용해시킨 결과 대두유의 품온이 30∼4 범위에서 가장 높은 용해도를