본 연구는 염소 도축공정 확립을 위해, 도축 과정 중 탕박(scalding) 및 박피(skinning)가 재래흑염소 등심의 저장 중 물리화학적 특성에 미치는 영향을 비교 분석하고자 수행되었다. 동일한 사양 조건에서 사육된 재래흑염소 6 두를 각각 탕박 및 박피 과정에 따라 도축한 후, 등심근을 채취하여 저장 기간 동안 이화학적 특성 변화를 관찰하여 재래흑염소에 적합한 도축 방법을 선택하고자 하였다. 그 결과, 탕박처리는 전반적으로 연도를 개선하는 데 효과적인 것으로 나타났으며, 박피 처리는 저장 중 보수력 유지에 우수하고, 색도의 선명도(a*, chroma)와 지질산화에 더 안정적인 특성을 보였다. 탕박은 소비자의 기호도 측면에서 유리한 부드러운 조직감을 제공할 수 있지만, 박피는 특히 위생적 안전성과 품질 균일성 확보 측면에서 더 바람직한 도축 방법으로 판단된다.

Efficient oocyte activation is a key step for the success of nuclear transfer in cloning. Ionomycin sequentially combined with 6-DMAP is now widely used to activate normal oocytes for analytical studies of oocyte activation and to activate reconstructed oocytes after nuclear transfer. The present study investigated sources of oocytes, duration of ionomycin and 6-DMAP, laser and electric stimulation in goat oocyte activation in order to optimize the protocols. Goat ovaries were collected in individual abattoirs during the breeding season and were delivered to the laboratory within 6 h in saline with 100 IU/ml streptomycin and 0.05 mg/ml penicillin. The oocytes were denuded from the cumulus cell by pipetting with 0.2% hyaluronidase in PBS at 20~22 hr post maturation. Oocytes with the polar body were selected and assigned to four groups for parthenogenetic activation. To examine the effect of duration of ionomycin treatment, oocytes after 20~22 hr of maturation were treated with 2.5 uM ionomycin for 1 or 5 min times and then cultured in 2 mM 6-DMAP for 2 or 4 hr. The activated oocytes were cultured in mSOF at in 5%, 5% and 90% multi incubator. Cleavage and blastocyst development was observed at 48 hr and day 8 of culture , respectively. Activation rates of oocytes exposed to ionomycin for 1 min(86.4%) were significantly higher than those treated for 5 min(74.3%) duration. This indicated that 1 min ionomycin treatment was most suitable for activation of goat oocytes. The duration of 6-DMAP treat duration was in 2 mM 6-DMAP for 2 hr after 1 min exposure to 2.5 uM ionomycin. The activation rate of oocytes incubated in 6-DMAP for 2 hour(82.5%) was significantly higher than those in oocytes treated with 4 hr(75.5%).