카렌듈라 추출물과 혼용되어 불리고 있는 메리골드 추출물을 인간섬유아세포에서 콜라겐 생성과 MMP-1 발현에 미치는 영향을 알아보기 위하여 HDF세포를 이용하여 세포독성 및 콜라겐 생성과 MMP-1 발현을 측정하였다. 실험 결과, HDF세포에 대한 메리골드와 카렌듈라 추출물의 5 ~ 100 μ g/mL 농도에서 80%이상의 세포 생존율을 나타내어 세포독성이 없었으며, 콜라겐 합성능 측정 결과, 두 추출물 모두 농도 의존적으로 콜라겐 생성능의 증가를 나타냈으며, 메리골드 추출물 100 μg/mL 농도에서 25%, 카렌듈라 추출물 100 μg/mL 농도에서 7% 콜라겐 생성능의 증가를 확인하였다. MMP-1 발현에 미치는 영향 실험 결과, 메리골드와 카렌듈라 추출물 모두 MMP-1 발현을 억제시키는 것을 확 인하였고, MMP-1 발현에 관련 있다고 알려진 p-JNK와 p-ERK의 인산화를 관찰한 결과, 메리골드 추 출물은 p-JNK와 p-ERK 신호전달 경로를 통화여 MMP-1 발현을 효과적으로 억제하는 것을 확인하였 다. 이와 같은 결과를 통해 메리골드 추출물의 주름 개선 효능을 확인하였고, 나아가 항노화 효능을 가 지는 화장품 원료로 활용 가능성을 확인하였다.

수경재배의 폐암면 처리문제를 해결하고 수입의존도가 높은 육묘용 상토 자재의 개발로 육묘산엽의 발전에 기여하고자 폐암면의 혼합비율을 달리한 플러그용 육묘상토를 조성하여 메리골드의 생육에 대한 효과를 조사하였다. 시판 육묘상토를 대조구로 하고, 시판 육묘상토에 이용되는 코코피트 대신에 폐암면을 10, 30, 50%로 혼합하여 혼합상토를 조제하여 50공 트레이에 파종하고 생육조사를 실시하였다. 메리골드의 발아율은 처리구간에 유의한 차이를 나타내지 않았다. 초장, 엽수, 경경, 엽면적과 지상부 및 지하부의 건물중 및 생체중은 시판상토와 폐암면 50% 혼합 처리구에서 양호하였다. 그러나 폐암면 30 및 10% 혼합처리구에서는 시판상토에 비해서 생육이 낮았는데 그 결과에 대해서는 정확한 원인을 밝힐 수 없었다. 본 실험에서는 폐암면을 플러그용 육묘상토의 자재로서 충분히 활용할 수 있다는 가능성을 보여 주었으며, 폐암면의 적정한 혼합비율에 대해서는 계속적인 실험이 필요한 것으로 생각되었다.

This study aimed to explore companion planting to improve vegetable productivity on extensive green roofs through urban agriculture with limited substrate depth. From May to July 2021, the study conducted on the rooftop to evaluate the effects of marigold (Tagetes patula) planting ratio on the growth and pest control of cabbage (Brassica campestris). The experiment plot measured 1 m in width × 1 m in length × 0.25 m in height and 0.2 m in substrate depth. Fifteen plots were planted in varying proportions of cabbage and marigold for three repetitions per treatment: cabbage control (CC), 2:1(C2M1), 1:1(C1M1), 1:2(C1M2), and marigold control (MC). We found that companion planting marigolds with cabbage significantly increased cabbage growth and reduced pest infestation. The study revealed that C1M1, when cabbage and marigold have the same proportion, is an efficient companion planting ratio. Companion planting, in which non-crop vegetation manages pests and increases crop productivity, improves natural pest control and preserves biodiversity on rooftop urban agriculture.

Background : Tagetes species which belong to Asteraceae show different characteristics including, bloom size, shape, color, plant size, and leaf shape. The color of Tagetes flowers ranging from white to dark orange is due to accumulation of different carotenoids, pathway intermediates, and amount of the same carotenoid. Methods and Results : The carotenoids were monitored in flower extracts from six cultivars of Tagetes that include three T. erecta cultivars, Discovery Orange (DO), Inca Orange (IO), and Inca Yellow (IY), and three T. patula cultivars, including Durango Bee (DB), Durango Yellow (DY), and Safari Red (SR) using HPLC analysis. It showed considerable differences in carotenoid composition depending on cultivars and types of carotenoids. The highest concentration of violaxanthin which represents orange color in plants was showed in IO, whereas the compound was not detected in DB, and DY. Yellow-colored cultivars such as IY, DB, and DY exhibited low levels of lutein. However, others that indicate orange color, DO, IO, and SR showed high levels of lutein. Also, similar pattern was found in the zeaxanthin measurements. α-carotene was significantly accumulated in SR compared to other cultivars. The highest amount of β-carotene was found in SR, followed by IO, IY, DO, DY and DB. Similarly, the highest and lowest amount of 9-cis-β-carotene was showed in SR and DB, respectively. Interestingly, all cultivars except SR in 13-cis-β-carotene showed the same pattern with β-carotene, but no detection indicated in SR. Conclusion : In this study, we determined the differences in carotenoid yields among six Tagetes cultivars. In total, seven carotenoids that include violaxanthin, lutein, zeaxanthin, α -carotene, β-carotene, 9-cis-β-carotene, and 13-cis-β-carotene were detected. Among them, all of the cultivars accumulated primarily lutein. In addition, contents of each carotenoid varied in these flowers depending on cultivars.

Background : Tagetes species which belong to Asteraceae show different characteristics including, bloom size, shape, color, plant size, and leaf shape. The color of Tagetes flowers ranging from white to dark orange is due to accumulation of different carotenoids, pathway intermediates, and amount of the same carotenoid. Methods and Results : The carotenoids were monitored in flower extracts from six cultivars of Tagetes that include three T. erecta cultivars, Discovery Orange (DO), Inca Orange (IO), and Inca Yellow (IY), and three T. patula cultivars, including Durango Bee (DB), Durango Yellow (DY), and Safari Red (SR) using HPLC analysis. It showed considerable differences in carotenoid composition depending on cultivars and types of carotenoids. The highest concentration of violaxanthin which represents orange color in plants was showed in IO, whereas the compound was not detected in DB, and DY. Yellow-colored cultivars such as IY, DB, and DY exhibited low levels of lutein. However, others that indicate orange color, DO, IO, and SR showed high levels of lutein. Also, similar pattern was found in the zeaxanthin measurements. α-carotene was significantly accumulated in SR compared to other cultivars. The highest amount of β-carotene was found in SR, followed by IO, IY, DO, DY and DB. Similarly, the highest and lowest amount of 9-cis-β-carotene was showed in SR and DB, respectively. Interestingly, all cultivars except SR in 13-cis-β-carotene showed the same pattern with β-carotene, but no detection indicated in SR. Conclusion : In this study, we determined the differences in carotenoid yields among six Tagetes cultivars. In total, seven carotenoids that include violaxanthin, lutein, zeaxanthin, α -carotene, β-carotene, 9-cis-β-carotene, and 13-cis-β-carotene were detected. Among them, all of the cultivars accumulated primarily lutein. In addition, contents of each carotenoid varied in these flowers depending on cultivars.