본 연구에서는 강원도 춘천지역에서 발생하는 환경 오폐수를 시료로 하여 미생물을 혼합하여 제조한 수질정화제를 실제 하천과 호수의 오염환경과 유사한 배양기내에서 생물학적반응을 시켜 오염물질의 정화정도를 측정하고 평가하였다. 사용된 미생물은 식물성 유산균(vegetable Lactobacillus fermentum), 효모(Saccharomyces cerevisiae)와 고초균(Bacillus subtilis)이다. 두 가지의 미생물혼합제를 투입하여 정화정도를 실험하였다. 실제 하천에 투입하여 정화되는 과정과 유사하게 반응을 시키고자 반응 온도는 21(±3)oC로 하였으며 완전교반을 진행하였다. 사용한 시료의 오폐수의 오염도와 미생물에 의한 정화정도를 평가하기 위하여 총질소 함량과 총인 함량을 측정하였다. V.L.F.와 S.C.의 혼합물로 구성된 혼합제의 경우 총질소함량을 감소시키는데 매우 효과적임을 보여주었으며, V.L.F., S.C.,와 B.S.로 구성된 혼합제의 경우 그 총인의 함량을 저감화하는데 효과적임을 알 수 있었다. 본 실험에서 얻어진 실험 데이터를 통하여 V.L.F.와 S.C.의 혼합제가 오폐수의 정화반응에서 나타나는 반응상수값을 도출하였으며 그 값은 0.178 day-1이었다. 본 연구의 결과를 바탕으로 저감화하고자 하는 성분에 따라 미생물의 종류와 그 혼합비를 조절하는 최적화과정이 필수적임을 알 수 있었으며 또한 곡선적합으로 도출한 반응상수값은 미생물을 이용하여 호수와 하천의 정화정도를 simulation함에 필요한 실험인자로서 응용이 가능하게 되었다.

This study attempted to utilize various groundcover plants in phytoremediation, using shade plants, which often have a high shade tolerance for shade urban space. Liriope platyphylla was grown in soil containing three of heavy metals, Zn, Cd, and Pb under four different concentrations (0, 100, 250 and 500 mg/kg) to determine the heavy metal accumulation characteristics and removing from soil. Total amounts of accumulated Zn in L. platyphylla were increased in accordance with increasing elevated Zn concentrations in soil, but the difference was not significant between Zn250 and Zn500. Cd accumulation, sharply increased in Cd100 and Cd250, but was reduced in Cd500. Increased Pb concentration in soil resulted a continuous increase in the total amounts of Pb accumulated in L. platyphylla. The total content of Zn in soil decreased by almost 50% in Zn100, almost 33% in Zn250 and 20% in Zn500 through growth of L. platyphylla over a period of 7 months. In the case of Cd, the concentration in soil, was decreased with 10% in Cd100, 10% in Cd250 and 33% in Cd500 through growth of L. platyphylla over a period of 7 months. This results indicate that more Cd was removed by planting L. platyphylla, compared to Zn or Pb.

In order to investigate the possibility for use to control environmental pollution, Kenaf( Hibiscus cannabinus L.) was used to obtain information of their growth and CO₂ response under different temperatures and CO₂ concentration. The highest percentage of germination and aboveground dry mass of Kenaf were found at 30℃ and 35℃ by 89.0% and 3.2g, respectively under different temperatures. The amount of CO₂ absorption and aboveground dry mass production of Kenaf were higher than those of maize during the whole growing period and the last sampling of aboveground dry mass of Kenaf and maize were 252.9g and 200.8g, respectively. The highest plant height was found at 400ppm by 131.0㎝ and the next was in the order of 600ppm by 129.3㎝, and 800ppm by 108.8㎝. Leaf area was higher in the order of 400ppm＞600ppm＞800ppm, whereas leaf dry mass was in the order of 800ppm＞600ppm＞400ppm under different CO₂ concentration, showing that leaf became thicker as CO₂ concentration was increased. Days from seeding to flowering became shorter by 13 days in 35/25℃ compared with 25/15℃ between two temperature regimes and they also became shorter as CO₂ concentration was increased. Aboveground dry mass was higher in 35/25℃ than that of 25/15℃ between two temperature regimes, while it was increased in the order of 800ppm＞600ppm＞400ppm as CO₂ concentration was increased. Temporal changes of leaf dry mass during growth period showed no difference between CO₂ concentration in 25/15℃, but the highest of it was found at 800ppm in 35/25℃. The highest temporal increase of root dry mass was found at 800ppm in 25/15℃, but 35/25℃ showed no difference between different CO₂ concentration.