In order to use as a new functional food material, we analyzed the chemical components including the organic compounds, minerals and Vitamin C of sorbus and acacia honey which were produced in South Korea. The condensed rate of methanol extraction in honey was 77% of sorbus honey and it was 93.06% of acacia honey. In the case of sorbus honey, main organic compounds that extract by organic solvents in GC-MS analysis were trichloromethane, propylcarbinol, cyclopentane, acetoxyethane, tetrasol, aziridine etc. and main aromatic compounds that extract by organic solvents in SPME analysis were Skelly solve, Benzaldehyde, Hyacinthin, Dodecanal, Lauraldehyde etc. Also, in occasion of acacia honey, main organic compounds were trichloromethan, Acetoxyethane, Hexanaphthene, acetidin etc. and main aromatic compounds were Hydrazomethan, Azulene, Cyclotrisiloxane, Hydrazine etc. Proximate composition was crude protein 0.54%, crude fat 0.44%, crude ash 0.25% in sorbus honey and crude protein 0.10%, crude fat 0.29%, crude ash 0.06% in acacia honey. Free sugar that analyze by HPLC was fructose 26.81%, glucose 20.42%, total sugars 47.23% in sorbus honey and fructose 48.52%, glucose 24.29%, total sugars 72.81% in acacia honey. Vitamin C was not detected in two sample honeys. Minerals by ICP analysis were detected total 18 kinds in sorbus honey, K 11.715 ppm>Na 7.857 ppm>Si 4.407 ppm>Ca 3.673 ppm etc. and total 22 kinds in acacia honey, Na 4.527 ppm>Si 3.420 ppm>K 3.091 ppm>Zn 1.482 ppm etc.

In order to use as a new functional food material, we analyzed the chemical components including the organic compounds, minerals and Vitamin C of cherry and acacia honey which were produced in South Korea. The condensed rate of methanol extraction in honey was 87.51% of cherry honey and it was 93.06% of acacia honey. In the case of cherry honey, main organic compounds that extract by organic solvents in GCMS analysis were trichloromethane, propylcarbinol, methacide, cyclopentane, tetrafinol etc. and main aromatic compounds that extract by organic solvents in SPME analysis were formyl trichloride, propanal, furfurylaldehyde, pyrazole, benzenecarbonal etc. Also, in occasion of acacia honey, main organic compounds were trichloromethan, Acetoxyethane, Hexanaphthene, acetidin etc. and main aromatic compounds were Hydrazomethan, Azulene, Cyclotrisiloxane, Hydrazine etc. Proximate composition was crude protein 0.33%, crude fat 0.15%, crude ash 0.47% in cherry honey and crude protein 0.10%, crude fat 0.44%, crude ash 0.06% in acacia honey. Free sugar that analyze by HPLC was fructose 37.05%, glucose 27.29%, total sugars 64.34% in cherry honey and fructose 48.52%, glucose 24.29%, total sugars 72.81% in acacia honey. Vitamin C was not detected in two sample honeys. Minerals by ICP analysis were detected total 25 kinds in cherry honey, K 9.762 ppm¤Si 5.628 ppm ¤Na 5.096 ppm¤Ca 2.224 ppm etc. and total 22 kinds in sacacia honey, Na 4.527 ppm¤Si 3.420 ppm¤K 3.091 ppm¤Zn 1.482 ppm etc.

Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of IL-1β, IL-6 and TNF-α as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated IκB-α degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of NF-κB activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, TNF-α, IL-6, IL-1β and MCP-1 via interruption of the NF-κB and MAPK/ATF2 signaling pathways.