본 연구에서는 메타분석을 통하여 heterofermentative과 homofermentative LAB가 사일리지의 품질과 호기적 안정성에 대한 효과를 분석하였다. 일련의 분석결과 접종균으로 사용된 젖산균의 종류에 따라서 사일리지의 품질이 다르게 나타남을 확인할 수 있었다. 특히 호기적 안정성에 있어서 heterofermentative LAB의 사용이 매우 중요함을 알 수 있었다. 본 연구결과는 사일리지의 제조 목적 및 사용방법에 따라서 접종균주를 다르게 선발하여야 하며, 사용방법에 따라서 사일리지 내 유기산 조성을 변화시킬 수 있는 발효조건이 필요함을 시사하고 있고, 또한 보다 정밀한 사일리지 발효기술에 대한 기초자료를 제공한다.
We compared between an automated most-probable-number technique TEMPO®TVC and traditional plating methods PetrifilmTM for estimating populations of total aerobic bacteria in various livestock products. 257 samples randomly selected in local retail stores and 87 samples inoculated with E. coli ATCC 25922, Staphylococcus aureus ATCC 12868 were tested in this study. The degree of agreement was estimated according to the CCFRA (Campden and Chorleywood Food Research Association Group) Guideline 29 and the agreement indicates the difference of two kinds methods is lower than 1 log base 10(log10). The samples of hams, jerky products, ground meat products, milks, ice creams, infant formulas, and egg heat formed products were showed above 95% in the agreement of methods. In contrast, proportion of agreement on meat extract products, cheeses and sausages were 93.1%, 92.1%, 89.1%, respectively. One press ham and five sausages containing spice and seasoning, two pork cutlets containing spice and bread crumbs, two meat extract product and two natural cheeses and one processing cheese with a high fat content, and one ice cream containing chocolate of all samples showed the discrepancy. Our result suggest that TEMPO®TVC system is efficient to analyses total aerobic bacteria to compare manual method in time-consuming and laborious process except livestock products having limit of detection.
This study investigated the influences of the addition of lactic acid bacteria inoculants and an enzyme during the crush processing of com silage on the nutritive value, nutrient intake and aerobic deterioration. The fermentation quality of the silages showed that although all of the silages were of good quality, the V-score of U was 91.0 points and significantly lower than those of other silages (P<0.01). On the chemical composition of silages, the crude protein concentration of U was 7.1%DM and significantly lower than those of other silages (P<0.05). On the digestibility, the nutritive value and nutrient intake did not significantly differ among the silages examined. After silo opening, a rapid rise in heat was seen from the 6th after opening for 11C38 and SLA, and from the 9th in U and SL, respectively.