The purpose of this study was to established the manufacturing process technology of an ultra-thin, multi-functional micro-implant(MFMI) that was optimized in fractured small animals, not mainly large animals. The test results showed a reckon type plate was more suitable than a straight plate because of excellent deformation energy. It was found that the structure of the hole and the screw could be fastened in all directions with the limited contact design of the threaded part cut off in 4 directions. It was possible to manufacture a reckon type plate reflecting the neck part of 2.0 mm width. It was confirmed that the plate hole could be stably and easily deformed and fastened without damaging the hole through the round type bending device.

Animal orthopedic implants are an area that has recently been come into the light due to growing number of single-person households and increasing of interest in pets. The purpose of this study was to investigate a method to design and manufacture a bushing in a desired direction and angle with the environment of use, while the bone joint plate could be stably deformed during animal fracture surgery. It was found that the bone joint plate was capable of deformation in all directions and was designed to be transformed to the desired angle without degrading the basic properties of bending strength. Also, it was found that the bushing-type bone joint plate could be controlled in multiple directions and angle at omni-directional movements(0°-360°) and 0°-10° moving forward, backward and side to side.

Multilayer Poly methyl methacrylate (PMMA)/ Poly vinyl alcohol (PVA) bone plates were fabricated using electrospinning and in vitro investigations were carried out for pre-clinical biocompatibility studies. The initial cellular cytotoxicity of the methacrylate (PMMA)/ Poly vinyl alcohol (PVA) bone plates was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using fibroblast-like L-929 cells. Cellular adhesion and differentiation studies were carried out using osteoblast-like MG-63 cells. As simulated body fluid (SBF) contains the same ionic concentration of body fluid and any bioactive material tends to deposit bone-like apatite on the samples surfaces into the SBF, in vitro bioactivity of the multilayer bone plates were investigated using SBF. We also studied the internal organization and tensile strength of the multilayer PMMA/PVA bone plates using micro-computed topography (μ-CT) and universal testing instrument (UTI, Korea) respectively. The cellular cytotoxicity study with MTT confirmed that the cellular viability was 78 to 90% which indicates good cyto-compatibility. Scanning electron microscopic findings revealed a good attachment and adhesion phenomenon of MG-63 cells onto the surfaces of the samples. Cellular differentiation studies also showed that osteogenic differentiation was switched on in a timely manner and affirmed along with that of the control group. Bone-like apatite formation on the surfaces was confirmed within 14 days of SBF incubation. Initial organizations of the multilayer PMMA/PVA bone plates were characterized as dense and uniform. The tensile strength of the post-pressing electronspun mat was higher than that of the pre-electronspun mat. These results suggest that a multilayer PMMA/PVA bone plate system is biocompatible, bioactive and a very good alternative bone plate system.