The antimicrobial susceptibility of the 41 Bordetella bronchiseptica isolates was tested by using the Kirby-Bauer agar disk diffusion method. The B. bronchiseptica isolates were found to be sensitive to amoxicillin/clavulanic acid (100%), gentamicin (100%), neomycin (100%) and amikacin (97.6%), whereas they were resistant to streptomycin (100%), trimethoprim/sulfamethoxazole (100%), penicillin (100%) and ampicillin (97.6%). All the B. bronchiseptica isolates resisted to at least 4 antimicrobial agents and totally 8 different combinations of multiple antibiotic resistance patterns were noted. All of the B. bronchiseptica isolates, except one, were simultaneously resistant to streptomycin, trimethoprim/sulfamethoxazole, penicillin and ampicillin. The observed antibiotic resistance is not plasmid mediated as plasmids were absent from all the B. bronchiseptica isolates.