Ainsliaea acerifolia leaves are registered with the Ministry of Food and Drug Safety as edible herbal materials in Korea, and research is underway to explore their potential in developing functional foods, cosmetics, and pharmaceuticals. In this study, we developed an analytical method using HPLC-DAD to quantify three key compounds—chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid—in A. acerifolia leaves extract. This method has been optimized and validated for specificity, accuracy, precision, limit of quantification (LOQ), and linearity. The correlation coefficients (r²) for the calibration curves exceeded 0.9962. The limits of detection (LOD) and quantification (LOQ) were 0.3012 and 0.9128 μg/mL for chlorogenic acid, 0.1182 and 0.3582 μg/mL for isochlorogenic acid A, and 0.2342 and 0.7098 μg/mL for 1,5-dicaffeoylquinic acid, respectively. The net recovery rates for accuracy testing were 105.13% for chlorogenic acid, 105.37% for isochlorogenic acid A, and 100.37% for 1,5-dicaffeoylquinic acid. All parameters assessed with this newly developed method fell within the acceptable ranges specified by ICH guidelines. These findings demonstrate that the method is robust and reliable for accurately identifying and quantifying chlorogenic acid, isochlorogenic acid A, and 1,5-dicaffeoylquinic acid in both routine analysis and large-scale extraction process of A. acerifolia leaves.

국화과에 속하는 단풍취의 추출물이 가지는 알츠하이머에 대한 효과를 알기 위하여 AChE에 대한 억제효과와 peroxy-nitrite 소거효과를 연구하였다. 단풍취 추출물은 AChE에 대한 억제효과로서 그 IC50가 73.4 μg/ml이었으며 peroxynitrite 소거효과에 관한 IC50는 8.60 μg/mL이었으므로 알츠하이머 질환에 유익할 것으로 판단된다. 단풍취에 가장 많이 함유된 성분인 3,5-dicaffeoylquinic acid 성분의 활성도 이와 유사한 활성을 보였으므로 단풍취 추출물의 효과는 주로 이 화합물의 활성에 따른 것으로 예상된다. Caffeoylquinic acid가 알츠하이머 질환에 효과있다는 보고가 있으므로 이 식물의 잎과 줄기 추출물에서 6종의 지표물질을 이용하여 정량법을 확립한 후 정량하였다. 그 결과 잎에서는 성분 함량이 25.1%를 차지하여 줄기의 함량보다 훨씬 높았다. 특히 잎 추출물 중 3,5-dicaffeoylquinic acid의 함량이 145.6 ㎎/g에 달하여 알츠하이머 질환 치료에 유익할 것으로 예상된다.

Five caffeoylquinic acids of Aster altaicus var. uchiyamae Kitamura (Compositae) leaves were identified using standard compounds by HPLC and determined as follows: 3,4-di-O-caffeoylquinic acid (4.92 ± 0.06 ㎎/g dried weight), 3,5-di-O-caffeoylquinic acid (3.95 ± 0.13 ㎎/g), 4,5-di-O-caffeoylquinic acid (1.39 ± 0.10 ㎎/g), 5-O-caffeoylquinic acid (chlorogenic acid, 8.05 ± 0.21 ㎎/g), 3-O-caffeoylquinic acid (4.97 ± 0.18 ㎎/g). The total content of five caffeoylquinic acids were calculated as 26.73 ± 0.26 ㎎/g dried weight while the percentage of the five compounds in the MeOH extract was calculated as 25.22 ± 0.25%. The IC50 value of the MeOH extract scavenging peroxynitrite (ONOO - ) was shown as 5.16 ± 0.15 ㎍/㎖.