본 연구는 자생 왕벚나무 조경수 및 가로수 수종 개발 연구에 기초 자료를 확보하고자 국내 선발된 9개 클론에서 개화기간 및 꽃의 형태적 특성을 조사하고 클론 간 유연관계를 분석하였다. 꽃의 형태적 특성을 조사하고 클론 간 차이를 비교한 결과 JJ02 클론이 총 13개의 꽃 특성 중 7개 특성이 상위그룹에 속하여 큰 경향을 나타낸 반면, JJ52 클론은 7개 특성이 하위 그룹에 속하여 비교적 작은 경향을 나타냈다. 조경수로 식재되는 벚나무는 개화기간이 길며, 꽃이 크고 개화량이 높은 수종을 선호하므로 JJ02 클론의 경우 가장 적합한 클론으로 나타났다. 주성분 분석을 실시한 결과 제 1주성분은 24.3%의 설명력이 있으며 꽃받침통 너비(FHW), 화편 길이(PL), 화편 폭(PW) 순으로 높은 상관을 나타내어 클론 간 꽃의 형태적 차이를 설명하는 주요 형질로 나타났다. 총화경(LP) 및 소화경(LPI) 길이와 높은 상관을 보인 제 2주성분, 20㎝ 개화지 당 꽃수(NIPF)와 화서 당 꽃수(NFPI)와 높은 상관을 보인 제 3주성분, 암술대 길이(SL)와 높은 상관을 보인 제 4주성분을 2차원 공간상에 배열해 본 결과 JJ02 클론이 우수한 것으로 나타났으며, 제 4주성분까지 설명력은 76.2%로 나타났다. 제 4주성분까지의 득점치를 새로운 변량으로 하여 군집분석을 실시한 결과 제 Ⅰ그룹인 JJ31 등 4개 클론, 그룹 Ⅱ은 JJ21 등 4개 클론, 그룹 Ⅲ은 JJ02 등 1개 클론으로 총 3개의 그룹으로 분류되었고, 클론 간 형태적 특성에 의해 소그룹으로 구분이 가능하다는 것을 알 수 있었다.

Plant regeneration from stems and leaves was carried out for the growth of biomass, hybrid poplar(Populus alba x P. glandulosa)clone, which has various uses for plant purification. Callus was well induced when stem and leaf tissues were cultured in 1.0 mg/L 2,4-D containing MS medium. Shoot regeneration was best induced by zeatin among growth regulators, and Plant regeneration was more regenerated in leaf-derived callus than in stem-derived callus. The growth of regenerated shoots at high and low concentrations of zeatin was similar to that of the control at low concentrations. As the concentration of growth regulators increased, the growth of regenerated shoots showed a big difference among individuals. Hybrid poplar showed color variation of plant stem in medium containing high concentration of growth regulator. Regenerated individuals were in vitro rooted in MS medium containing 0.5 mg/L IBA after 2 weeks of culture. and transferred to the greenhouse for acclimatization. The study is believed to be widely used for the induction of in vitro variants through organogenesis.

There are increasing interests in developing methods specifically detecting pathogenic Bursaphelenchus xylophilus. In order to develop a detecting method for B .xylophilus, at first we generated monoclonal antibodies (MAbs) specific to B. xylophilus, discriminating from other pine tree resident nematodes. Among 2304 hybridoma fusions screened. We finally selected a MAb clone, 9F10 and used for further study. To identify the antigenic target of MAb-9F10, we employed several biochemical methods such as SDS-PAGE, 2 dimensional electrophoresis, anion exchange chromatography, and immunoprecipitation to separate and isolate an antigenic target. Proteins from above methods were analyzed via nano-LC-ESI-Q-IT-MS. Peptides of GaLECtin were always detected from several proteomic analyses, suggesting that GaLECtin is the antigenic target of MAb-9F10.

곰피추출물의 CCD-986sk cell line monolayer (human fibroblast, KCBL-21947)에 대한 피부세포 생리활성효과를 측정하고, 또한 곰피추출물의 Clone M-3 mouse melano-cyte cell line에 대한 melanin formation 저해효과를 측정하기 위해 in vitro레벨에서 실험을 실시하였다. 곰피는 다년생 갈조류의 일종으로 이 종은 한국 연안해역에서 중요한 1차생산자의 역할을 담당하고 있는

Somatic cell nuclear transfer in cattle has limited efficiency in terms of production of live offspring due to high incidence of fetal failure after embryo transfer to recipients. Such low efficiency of cloning could possibly arise from abnormal and poorly developed placenta. In the present study the placental proteome in late pregnancy established from in vitro fertilization (IVF) and nuclear transfer (NT) was analysed. Proteome alternation was tested using two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI- TOF). Comparing placenta from NT embryos to those from IVF counterparts, significant changes in expression level were found in 18 proteins. Of these proteins 12 were not expressed in NT placenta but expressed in IVF counterpart, whereas the expression of the other 6 proteins was limited only in NT placenta. Among these proteins, cytokeratin 8 and vimentin are considered to be involved in regulation of post-implantation development. In particular, cytokeratin 8 and vimentin may be used as makers for placental development during pregnancy because their expression levels changed considerably in NT placental tissue compared with its IVF counterpart. Data from 2-DE suggest that protein expression was disorientated in late pregnancy from NT, but this distortion was eliminated with progression of pregnancy. These findings demonstrate abnormal placental development during late pregnancy from NT and suggest that alterations of specific placental protein expression may be involved in abnormal function of placenta.

In order to search for anti -melanin formation agents from Korean medicinal herbs, we selected 21 Korean medicinal herbs, based on a review of Korean traditional medicine books and the recommendations of Korean traditional medical doctors. We tested for i

Background : Ramie, Boehmeria nivea (L) Gaud, is a bast fiber plant of the nettle family (Urticaceae) and originated in Asia. We have taken an active interest in ramie breeding for edible ramie, and consequently select "Yeonggwang No.1" with high yielding and good quality for edible leaves in 2015. Methods and Results : "Yeonggwang No.1" is a new ramie variety for edible leaves, which was derived from the plants of the population for pure clone isolation from 2013 to 2014, and progressively for specific character and yield trial in 2014 to 2015. The yield trials were carried on the field and the greenhouse with two replications, respectively. The plant height of "Yeonggwang No.1" is about 140 ㎝ with 6 ㎝ higher than that of check variety "Seobang" The leaves are heart-shaped, with large size, and finely serrated margins. The color of the upper side of the leaf is dark green, and silvery white on the under surface. The flowers are light purple in color and are borne in declinate clusters in the axils of the leaves. The average SLW (specific leaf weight) was 8.56 ㎎/㎠, which was weight lighter than that of check "Seobang", but chlorophyll content measured by a Chlorophyll meter, SPAD-502 was 12.3 SPAD higher than that of "Seobang". Fresh leaf yield was the annual total 1,578 ㎏/10 a in the field, and 2,479 ㎏/10 a in the greenhouse, which was 98% level of "Seobang" in the field, and increased by 10% than check "Seobang" in the greenhouse. The fresh leaves yield of "Yeonggwang No.1" tended to increased in changes according to time more than "Seobang". Conclusion : As a new variety, Yeonggwang No.1 can be harvested leaves several times annually, with the yield range of 1,578 – 2,479 ㎏/10 a, having 72.3% moisture, 6.61% crude protein, 987 ㎎ calcium, 5.27 Fe in its leaves. These results seem to indicate that there is considerable scope for increasing the fresh leaf yield of ramie and improving the processing quality by means of selection from the large and comparatively unexplored pool of variation, which should be obtainable from this heterozygous plant.