This study aimed to investigate whether bacterial ghosts (BGs), empty cell envelopes of a gram-negative bacterium, delivering envelope protein domain III (EDIII) of dengue virus (DENV) serotype 2 could induce protective immune responses against dengue infection. In this study, we constructed Salmonella Typhimurium BGs expressing and delivering EDIII (BG-EDIII) and evaluated these ghosts for their immunogenicity studies in C57BL/6 mice. Our results demonstrated that the mice vaccinated once orally with BG-EDIII followed by an intramuscular boosting with a recombinant EDIII protein elicited significantly higher humoral and cell-mediated immune responses compared to the BGs alone vaccinated group (p<0.001). Upon challenge with DENV2, significantly lower viral load and liver damage was observed in BG-EDIII vaccinated group than BGs alone control group (p<0.05). The outcomes of this study revealed the ability of BG- EDIII to stimulate immune response with no observable damage to the vital organs.

Markov envelope as a theoretical solution of the parabolic wave equation with Markov approximation for the von Kármán type random medium is studied and approximated with the convolution of two probability density functions (pdf) of normal and gamma distributions considering the previous studies on the applications of Radiative Transfer Theory (RTT) and the analysis results of earthquake records. Through the approximation with gamma pdf, the constant shape parameter of 2 was determined regardless of the source distance ro. This finding means that the scattering process has the property of an inhomogeneous single-scattering Poisson process, unlike the previous studies, which resulted in a homogeneous multiple-scattering Poisson process. Approximated Markov envelope can be treated as the normalized mean square (MS) envelope for ground acceleration because of the flat source Fourier spectrum. Based on such characteristics, the path duration is estimated from the approximated MS envelope and compared to the empirical formula derived by Boore and Thompson. The results clearly show that the path duration increases proportionately to ro 1/2-ro 2, and the peak value of the RMS envelope is attenuated by exp (-0.0033ro), excluding the geometrical attenuation. The attenuation slope for ro≤100 km is quite similar to that of effective attenuation for shallow crustal earthquakes, and it may be difficult to distinguish the contribution of intrinsic attenuation from effective attenuation. Slowly varying dispersive delay, also called the medium effect, represented by regular pdf, governs the path duration for the source distance shorter than 100 km. Moreover, the diffraction term, also called the distance effect because of scattering, fully controls the path duration beyond the source distance of 300 km and has a steep gradient compared to the medium effect. Source distance 100-300 km is a transition range of the path duration governing effect from random medium to distance. This means that the scattering may not be the prime cause of peak attenuation and envelope broadening for the source distance of less than 200 km. Furthermore, it is also shown that normal distribution is appropriate for the probability distribution of phase difference, as asserted in the previous studies.

이 논문은 드럼 세트를 구성하는 악기들 중 스네어 드럼에 사용되는 진동제어장치의 크기와 질량의 변화에 따라 음량과 음색 및 엔벨로프가 어떻게 변화되는지에 대해 연구하였다. 스네어 드럼을 사용하여 연주하는 드럼 연주자 및 음향 엔지니어들에게 진동제어장치의 변화에 따라 도출되는 소리의 특성을 제공하여 표현하고 싶은 스네어 음색을 구사할 수 있는 정보를 제공하는 목적을 가지고 있으며 스네어 드럼의 음색을 구사하는 능력이 향상된다는 것은 음악의 장르와 스타일에 따라 연주자 및 음향 엔지니어의 표현 능력이 향상될 수 있는 긍정적 영향을 끼칠 수 있다. 실험 결과 진동제어장치를 사용하여 면적과 질량의 변화를 준 환경에서 라우드 니스의 변화는 발생하지 않았으며, 주파수 응답의 변화는 미미한 수준이었고, 엔벨로프의 길 이에서는 큰 변화가 발생하였다. 실험과정에서 실제로 듣는 청감상의 스네어 드럼 음색 변화는 매우 크게 나타났으며 특히 질량보다는 면적의 변화에 따른 실험환경에서 큰 음색 변화가 있었다. 결과적으로 진동제어장치는 실질적으로 엔벨로프에만 관여하며, 스네어 드럼은 엔벨 로프의 길이가 짧아 엔벨로프의 앞부분에서 발생하는 음색이 더 중요하다는 일부 인식과 달 리, 엔벨로프의 뒷부분도 인간이 지각하는 음색에 큰 영향을 준다는 연구들이 도출되었다.

Determining the infection history of living organisms is essential for understanding the evolution of infection agents with their host, particularly for key aspects such as immunity. Viruses, which can spread between individuals and often cause disease, have been widely examined. The increasing availability of fish genome sequences has provided specific insights into the diversity and host distribution of retroviruses in fish. The shortspine spurdog (Squalus mitsukurii ) is an important elasmobranch species; this medium-sized dogfish typically lives at depths of 100~500 m. However, the retroviral envelope polyprotein in dogfish has not been examined. Thus, the aim of the present study was to identify and analyze the retroviral envelope polyprotein in various tissues of dogfish. The 1334-base pair full-length novel cDNA of dogfish envelope polyprotein (dEnv) was obtained by 3' and 5'-rapid amplification of cDNA end analysis from S. mitsukurii. The open reading frame showed a complete coding sequence of 815 base pairs with a deduced peptide sequence of 183 amino acids that exhibited 34~50% identity with other fish and bird species. It was also expressed according to reverse transcription and real-time polymerase chain reaction in the kidney, liver, intestine, and lung, but not in the gill. This distribution can be assessed by identifying and analyzing endogenous retroviruses in fish, which consists of three main genes: gag, pol and env. Dogfish envelope polyprotein sequence is likely important in evolution and induces rearrangements, altering the regulatory and coding sequences. This is the first report of the identification and molecular characterization of retroviral envelope polyprotein in various tissues of S. mitsukurii.

Most of the stars in the Galaxy are in binary systems. Binaries should be possible as the hosting stars of planets. Searching for planetary companions to binaries, especially evolved close binary stars, can provide insight into the formation and the ultimate fate of circumbinary planets and shed light on the late evolution of binary stars. In order to do this, we have chosen some post common envelope binaries including sdB-type eclipsing binaries and detached WD+dM eclipsing binaries as our targets and monitored them for several years. In this paper, we will present some of our new observations and results for three targets, NSVS 07826147, NSVS14256825 and RR Cae.

The common-envelope process is a complicated phase in binary evolution. A lot of effort has been dedicated to study the common-envelope stage, but many questions related to this process are yet to be answered. If one member of the binary survives the common-envelope phase, the binary will emerge as a white dwarf accompanied by a low-mass main sequence star in close orbit, often referred as a post common-envelope binary (PCEB). SDSS J0745+2631 is among the list of newly found PCEBs from the Sloan Digital Sky Survey (SDSS). This star is proposed to be a strong eclipsing system candidate due to the ellipsoidal modulation in its light curve. In this work, we aim to conrm the eclipsing nature of SDSS J0745+2631 and to determine the stellar and orbital parameters using the software Binary Maker 3.0 (BM3.0). We detected the primary eclipse in the light curve of SDSS J0745+2631 in our follow-up observation from January 2014 using the ULTRASPEC instrument at the Thai National Observatory. The data obtained on 7th and 8th January 2014 in g filter show an evident drop in brightness during the eclipse of the white dwarf, but this eclipse is less prominent in the data taken on the next night using a clear filter. According to our preliminary model, we find that SDSS J0745+2631 hosts a rather hot white dwarf with an eective temperature of 11500K. The companion star is a red dwarf star with a temperature of 3800K and radius of 0.3100 R⊙. The red dwarf star almost fills its Roche lobe, causing a large ellipsoidal modulation. The mass ratio of the binary given by the Binary Maker 3.0 (BM3.0) model is M2/M1 = 0.33.

I selected a hypermarket as a research target by investigating energy consumption and total area of each 11 hypermarkets and suggested energy saving key elements by analyzing the cooling and heating loads. I completed a calibrated simulation model by searching literature, site survey and dynamic simulation tool. I separated the energy-saving elements into architectural and facility sections by searching domestic standards as well as theses, and applied those elements to the calibrated simulation to calculate the energy consumption. Then I suggested energy saving elements that suitable for the hypermarkets by comparing each energy consumption of architectural and facility sections.

The silkworm-baculovirus expression system has distinct advantages, such as a high yield and safe usage in vertebrates. Here, we report a novel strategy for the large-scale production of a classical swine fever virus (CSFV) envelope glycoprotein E2 in the larvae of a baculovirus-infected silkworm, Bombyx mori. We constructed a recombinant B. mori nucleopolyhedrovirus (BmNPV) that expressed recombinant polyhedra together with the N-terminal 179 amino acids of CSFV E2 (E2ΔC). BmNPV-E2ΔC-infected silkworm larvae expressed native polyhedrin and approximately 44-kDa fusion protein that was detected using both anti-polyhedrin and anti-CSFV E2 antibodies. Electron and confocal microscopy both demonstrated that the recombinant polyhedra contained both the fusion protein and native polyhedrin were morphologically normal and contained CSFV E2ΔC. The CSFV E2ΔC antigen produced in BmNPV-E2ΔC-infected silkworm larvae reached 0.68 mg per ml of hemolymph and 0.53 mg per larva at 6 days post-infection. Six-week-old female BALB/c mice that were immunized with the E2ΔC protein purified from solubilized recombinant polyhedraelicited CSFV E2 antibodies, which indicated that the CSFV E2ΔC protein from recombinant polyhedra was immunogenic. The virus neutralization test showed that the serum from mice that were treated with E2ΔC protein from recombinant polyhedra contained significant levels of virus neutralization activity. These results demonstrate that the present strategy can be used for the large-scale production of CSFV E2 antigen.

Classical swine fever virus (CSFV) envelope glycoprotein E2 is the main target for inducing neutralizing antibodies and protective immunity in swine. Here, we report a novel strategy forthe large-scale production of a CSFV E2 subunit vaccine that demonstrates a high immunogenic capability in the larvae of a baculovirus-infected silkworm, Bombyx mori. We constructed a recombinant B. mori nucleopolyhedrovirus (BmNPV) that expressed recombinant polyhedra together with the N-terminal 179 amino acids of CSFV E2 (CSFV E2ΔC). BmNPV-E2ΔC-infected silkworm larvae expressed an approximately 44-kDa fusion protein that was detected using both anti-polyhedrin and anti-CSFV E2 antibodies. Electron and confocal microscopy both demonstrated that the recombinant polyhedra were morphologically normal and contained CSFV E2ΔC. The CSFV E2ΔC antigen produced in BmNPV-E2ΔC-infected silkworm larvae reached 0.68 mg per ml of hemolymph and 0.53 mg per larva at 6 days post-infection. Mice that were immunized with the granule form of recombinant polyhedra or the soluble form of the fusion protein elicited CSFV E2 antibodies, which indicated that the recombinant polyhedra carrying CSFV E2ΔC were immunogenic. The virus neutralization test showed that the serum from mice that were treated with recombinant polyhedra or the soluble form of the fusion protein contained significant levels of virus neutralization activity. These results demonstrate that the present strategy can be used for the large-scale production of CSFV E2 antigen and that the recombinant polyhedra containing CSFV E2ΔC as a granule antigen can be used as a potential subunit vaccine against CSFV.

The study of cornified cell envelope has been used to investigate the differentiation factors and to advance oral carcinogenesis, CE of human oral keratinocytes are in wet condition as saliva containing many proteases, growth factors, and many kinds of bacteria, The analysis of CE in Immortalized human oral keratinocyte(IHOK) derived from normal human oral keratinocyte(NHOK) will be used to study the pathogenesis of oral squamous cell carcinoma, The purpose of this study was to analyze the amino acid component derived from CE of cultured NHOK and IHOK, It will be helpful to study the role of transfected E6/E7 gene in forming CE, and to examine the pathogenesis of oral squamous cell carcinoma, After primry culture of NHOK, IHOK were cultured in KBM bullet kit at 370C under 95% C02 incubator, Growth curve according to calcium concentration, cornified cell envelope measurement(CEM), and protein chemistry for amino acid component of CE were done(Mena :f::SD) , respectively. The obtained results were as follows, lHOK showed small areas of stratification, more compact, with irregular border and tightly apposed cells in 1,2 mM Ca++, Cornified cell envelope exhibited an aggregated group of empty space surrounded by the remained cell membrane, During the terminal differentiation in cultured NHOK and IHOK, insoluble cornified cell envelope formation was increased, CEM of NHOK was about 4 folds than that of lHOK under high calcium, Amino acid component of both groups showed Pro/Glu(SPR) , Gln/Glu(lnvolucrin) , and Gly(Loricrin) in descending order, From the aboving results, ít was suggested that when the terminal dífferentiation in cultured NHOK and IHOK, major amino acid component of CE in cultured lHOK was the same to that of cultured NHOK, It was thought that E6 and E7 gene should be involved in preventing the differentiation and proliferation of IHOK from making CE,