GRP관은 연성관으로 분류되며, 지중에서 지반과 함께 외부하중에 저항하는 상호거동을 한다. 또한 국내 설계기준에서는 GRP관의 설계에 대한 명확한 규정을 제시하지 못하고 있으나, AWWA M 45 및 ASTM D 2412에서는 지중매설 GRP관의 관변형에 대한 주요설계변수를 관강성, 지반반력계수, 기초각 등으로 규정하고 있다. 이 연구에서는 지중매설된 연성관의 구조적 거동을 파악하기 위하여 기존 연구에서 수행한 연구결과와 AWWA M 45에서 제시하고 있는 설계식을 이용하여, 관강성, 지 반반력계수, 기초각계수를 변수로 지반-관 상호작용 특성을 검토하였다. 검토결과 지중매설 GRP관의 구조적 거동에 가장 큰 영향을 미치는 설계변수는 지반반력계수이고, 기초각은 180°로 시공하여야 하며, 특히 대구경관에서는 지반의 영향이 더욱 중요한 것으로 나타났다. 따라서 GRP의 안전성을 확보하기 위해서는 시공과정에서 되메움토에 대한 다짐도, 상대밀도, 흙의 종류에 대한 세부적인 규정과 관리가 필요하며, 특히, 헌치부에 대한 시공관리가 이루어져야 할 것으로 판단된다.

Melatonin has an important role as anti-oxidative effect and reducing of endoplasmic reticulum(ER)-stress on oocyte maturation and embryo development. Under ER-stress condition, unfolding protein response (UPR) is a defence mechanism in mammalian cells. Recently, regulation of UPR signaling genes are involved in oocyte maturation, embryo development and female reproduction. However, there is no report on the role of melatonin for UPR signaling and ER-stress mediated apoptosis during pig oocyte maturation progression. Moreover, the changes of UPR genes expression according to the porcine oocyte maturation is not yet fully understood. Here, we investigated the changes of UPR signal (BIP/GRP78, ATF4, p90/p50ATF6, and XBP1) and ER-stress apoptotic factor CHOP genes expressions in porcine oocyte maturation by Western blot and RT-PCR analysis. During oocyte maturation, UPR marker and CHOP genes expressions were significantly increased in matured oocytes or cumulus-oocyte complexes (COCs). UPR markers expressions were significantly increased by ER-stress inducer, tunicamycin (Tm), treated (1, 5, 10 μg/ml) groups in a dose-dependent manner compared with control group. To confirm the reducing of ER-stress by melatonin (0.1 μM), we were compared to the effects of ER-stress inhibitor, TUDCA (200 μM), after pre-treated Tm (5 μg/ml) for 22 h maturation. Expressions of UPR markers and meiotic maturation were recovered by melatonin (0.1 μM) in COCs. And, we observed the role of Grp78/Bip as UPR signaling beginning marker using siRNA. In result, reduction of Grp78/Bip gene expression by siRNA was induced the inhibition of oocyte maturation (32.5±10.1 vs control; 77.8±5.3), and p50ATF6 protein level was significantly decreased (p<0.001) in cultured COCs for 44 h. In addition, these results were recovered through the addition of melatonin (0.1 μM) or TUDCA (200 μM) in maturation medium. These results demonstrated that the regulation of UPR signaling via Grp78/Bip gene induction plays a critical role in porcine oocyte maturation in vitro. Furthermore, this present study first confirmed a functional link between inhibition effect of ER-stress by melatonin and regulating of UPR signaling in porcine oocyte maturation. In conclusion, melatonin improves the oocyte maturation and cumulus cells expansion of COCs through the regulation of UPR signal pathway by BIP/GRP78 against the ER-stress during porcine oocyte maturation periods.

The sweetpotato whitelfy Bemisia tabaci distribute worldwide and infests more than 500 species of plants. To determine nutritional stress of whiteflies at molecular level, we identified a full cDNA of glucose regulated protein 78 (grp78) which is known to be respond to nutritional restriction in vertebrate species. GRP78 of B. tabaci was highly conserved motifs of the HSP70 family and the C-terminal motif of KDEL characteristic of endoplasmic reticulum-specific HSPs. Real-time RT-PCR analysis showed that the grp78 level was not changed by thermal stress treatment from 4°C to 40°C for 1 h. However, the grp78 level was proportionally increased to the ingestion of a sucrose solution ranging in concentrations from 0% to 30% in a Parafilm feeding chamber. In addition, the grp78 levels were various by the ingestion of leaves of 10 different plants for 24 h; its level was higher with eggplant and pepper but lower with rice and apple. Our study suggests that the grp78 may have a role for cellular chaperones in relation to nutritional uptake of B. tabaci.

Glucose-regulated protein 78 (GRP78) is a member of the heat shock protein 70 (HSP70) family that is specific to endoplasmic reticulum (ER). It is known as chaperones and signaling regulators that respond to ER stresses in vertebrates. However, its function in invertebrates, including insects, is uncertain. Here we determined a full cDNA sequence and the expression patterns of grp78 of Aphis gossypii, which is a major pest of numerous crop plants worldwide. Its cDNA had highly conserved motifs of the HSP70 family and the C-terminal motif of KDEL characteristic of ER-specific HSPs. It showed 98.5% identity with the GRP78 of the pea aphid Acyrthosiphon pisum. Real-time RT-PCR analysis showed that the grp78 level was higher in the fourth instar nymph than in the younger instar and adult stages. Its level was not affected by thermal stress of 10 to 40°C for 1 h. The grp78 level was proportional to the ingestion of a sucrose solution ranging in concentration from 0% to 30% in a Parafilm feeding chamber. In addition, the grp78 level varied among aphids feeding on leaves from 14 different host plants for 24 h; it was higher with eggplant and pepper but lower with pigweed and tobacco than any other plants. Our study suggests that the grp78 level is regulated by nutritional condition of A. gossypii.

Cotton aphid infests more than 700 plants and a major pest of various horticultural crops worldwide. The glucose regulated protein 78 (GRP78) is a member of heat shock protein 70. Its expression is associated with the nutritional changes as well as environmental stresses. The full sequences of grp78 cDNA of Aphis gossypii was determined. It had conserved motifs of hsp genes and terminated in KDEL which is common to GRP78. Quantitative realtime PCR showed that its level was changed during development and also upregulated by starvation. However, its level was not much changed by heat stress. The level of grp78 can be use to understand nutritional physiology on insects.

Glass fiber reinforced thermosetting polymer plastic (GRP) is widely used in the construction industries due to the advantages of their superior mechanical and physical characteristics. Most of pipes are installed for long-term purposes and they should be safely installed in consideration of installation conditions because there are unexpected various terrestrial loading conditions. In this paper, we present the result of investigation for 253 days pertaining to the structural behavior of flexible pipes buried underground. From the buried test results, we predicted long-term, up to 60 years, ring deflection of GRP pipes buried underground based on the method suggested by the existing literature. It was found that the GRP flexible pipe is appropriate because 5% ring deflection limitation of 60 years could be satisfied.

Glass fiber reinforced plastic (GRP) pipes buried underground are attractive for use in harsh environments, such as for the collection and transmission of liquids which are abrasive and/or corrosive. In this paper, we present the result of investigation pertaining to the structural behavior of GRP flexible pipes buried underground. In the investigation of structural behavior such as a ring deflection, experimental and analytical studies are conducted. In addition, vertical ring deflection is measured by the field test and finite element analysis (FEA) is also conducted to simulate behavior of GRP pipe buried underground. Based on the results from the finite element analyses considering soil-pipe interaction the vertical ring deflection behavior of buried GRP pipe is predicted. In addition, analytical and experimental results are compared and discussed.

Interferon induced transmembrane protein-1 (IFITM1) is one of transmembrane protein which is differentially expressed in uterus during estrus cycle and pregnancy, that IFITM1 gene is highly expressed in estrus stage by the effect of estrogen, and in parturition by the effect of PGF2 alpha. This genes are also up-regulated in cells with hyperactivation of the WNT/β-catenin signaling pathway. In this study, to identify a function of IFITM1, the binding partner of IFITM1 were determined using immunoprecipitation and LC- MASSMASS methods. 1, 3 and 5 ug of polyclonal anti-IFITM1 antisera were used for immunoprecipitation, and the 75 kDa of specific band was detected in silver stained polyacylamide gel. This band were chracterized using LC-MASS-MASS, and revealed this band is glucose regulated protein 75 (GRP75) which binds to p53 and inhibits the p53 action in nucleus. To identify the localization of GRP75 in cells, immunocytochemical approach has been applied, and GRP75 is expressed in mitochondria of L929 murine connective tissue cells. Co-localization study between IFITM1 and GRP75 in L929 cell identified that these two proteins were closely expressed in mitochondria. Although the role of the interaction of these two protein need to be clarified in various biological phenomena, this data suggest that close interaction of IFITM1 and GRP75 may regulate cellular functions in uterus on sets of estrus cycle and pregnancy.

This study investigates the elastic behavior characteristics of GRP pipes reinforced with trapezoidal ribs instead of rectangular ribs. Han(2006) proposed GRP pipe reinforced with rectangular ribs in 2006, to promote buckling strength of the pipe. But it is more difficult to reinforce pipe with rectangular ribs than external corrugated ribs, because of pipe manufacturing process referred to as filament winding. Therefore, This study investigated the elastic behavior characteristics of the GRP pipe reinforced with trapezoidal ribs for simplifying analysis approach of external corrugated ribs.

GRP (Glass fiber reinforced polymer plastic) pipes are classified into flexible pipe category because of the structural characteristics of GRP pipes buried underground. Moreover, the ring deflection of GRP pipe is affected by the certain factors such as pipe stiffness, the modulus of soil reaction of backfill materials, and bedding conditions. In this study, we investigated the structural characteristics of the GRP pipes buried underground considering the soil-pipe interaction including bedding angle, pipe stiffness, and soil density used as a backfill material. From this parametric study, it was found that the soil density used for the backfill is the most important factor relating to the ring deflection of the buried GRP pipe.

이 연구에서 GRP 관의 하중-처짐 거동을 조사, 보고하였다. 지중매성 GRP관은 높은 내화학성, 높은 부식저항성, 경 량성, 관표면의 매끄러움, 지반-관의 상호작용 고려에 따른 경제성 등의 탁원한 역학적, 물리적 특성들로 인해 건설 현장에서 광범위하게 사용되고 있다. 지중에 매설되는 연성관을 설계하기 위해서는 ASTM D 2412 (2010)에 따라야 한다. ASTM D 2412 (2010)에 따라 설계할 경우, 관의 원강성 (PS)을 편평시험에 따라 먼저 결정해야 하는데, 이 시 험이 귀찮고 노동력을 필요로 한다. 이러한 문제를 해결하기 위해 UTM에 설치된 형태의 GRP관의 하중-처짐 거동 을 유한요소법에 따라 모사하였으며, 유한요소법에 의한 모사에는 재료의 탄성계수와 단면의 기하학적 치수 등 기 초적인 자료를 사용하였다. 이와 같은 연구로부터, 관재료가 관의 단면내에서 비교적 일정하지 않음에도 불구하고 수직방향의 관변형이 3%와 5%가 발생할 경우, 편평시험과 수치해석적 연구 결과가 15%이내의 차이로 하중의 예측 이 가능함을 알 수 있었다.

최근 지중매설 유리섬유복합관(GRP관)은 유해한 환경에서 뛰어난 성능을 보유하고 있어 하수관거용으로서 사용이 증가 되고 있다. 또한 지중에 매설되어 있는 조건에서 기존의 콘크리트관 등 강성관에 비해 구조적 성능이 뛰어나 다. 지중매설 GRP관은 주로 상부에 작용하는 상부토압과 활하중에 의한 압축응력에 의해 원주방향으로 변형이 일 어나게 된다. GRP관의 구조적 거동은 매설토와 주변의 지반의 성질에 따라 다르게 설계되어야 한다. GRP관의 설 계는 Spangler 의 변형량 계산식을 Watkins에 의해 수정되어 사용되고 있다. 이 연구에서는 Watkins의 관변형 추정 식에 GRP관의 재료적 특성을 고려하여 관변형량을 예측하였다.

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)- related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: 32.5±10.1% vs control: 77.8±5.3%). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, 200 μM) and melatonin (0.1 μM), in BIP/ GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, p-eIF2α, eIF2α, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.

GRP(Glass Reinforced Plastic) 파이프는 GRP 재료 자체의 고강도, 고내구성으로 인해, 구조용 원형강관을 대체할 수 있을 만큼 그 적용성이 넓을 것으로 판단된다. 특히, 주철관, 콘크리트관로를 대체할 상하수도관로로써 뿐 아니라 ICH-CFT기둥, 풍력타워 등의 고내구성과 고강도가 필요한 구조분야에서 새로운 대체 구조로서 주목받고 있다. 하지만, GRP 파이프는 고강도의 박판구조로 제작된 연성관으로써 압축력이 지배 인자로 작용하는 구조물이기 때문에 좌굴 문제가 중요한 요소이다. 국내에서 고안된 GRP 파이프의 강성을 향상시키는 대표적인 방법으로는 각형 rib로 보강하는 방법, 파형으로 제작하는 방법, Mesh로 보강하는 방법 등이 있으며, 이에 대한 실험적 해석적인 연구가 수행되어 왔다. 이들 중 한택희 등(2007)에 의해 연구되어진 각형 리브로 보강된 GRP 파이프는 그 적용성이 넓어 상하수도 파이프뿐 아니라, ICH-CFT기둥의 내부 튜브 등으로의 적용이 가능하다. 한편, GRP 파이프는 원통형 몰드에 유리장섬유를 원주방향, 길이방향으로 배치하여 각각 필라멘트 와인딩하여 적층하는 방법으로 제작된다. 이러한 제작방법으로 한택희 등(2007)에 의해 연구되어진 각형 리브로 보강된 GRP 파이프를 실제로 제작하였을 때는, 반복적인 필라멘트 와인딩으로 인해 각형보다는 곡선을 이루는 외부 파형의 형태로 만들어지며, 각형 리브와 비교하였을 때, 리브와 파이프 연결 부분에서 응력집중 등의 영향이 서로 상이하여 구조적인 거동이 다를 수 있을 것이라 판단된다. 본 연구에서는 각형 리브에 비해 제작이 쉬운 외부파형 리브로 보강된 GRP 파이프의 탄성 좌굴거동분석을 위한 매개변수연구에 초점을 맞추었다. 매개변수로는 직경에 대한 외부 파형 리브의 높이비(h/D), 폭비(w/D), 배치 간격비(s/D)를 두어, 각 변수에 의한 좌굴강도의 증가를 한택희 등(2007)에 의해 연구된 각형 리브로 보강된 좌굴강도와 비교하여 분석하였다. 또한, 기존에 제안된 외압을 받는 리브로 보강된 GRP파이프의 좌굴강도식을 보완한 새로운 좌굴 강도식을 제시하기 위한 기초자료를 제공하고자 한다.