재래흑염소는 다른 품종과의 유연관계, 기원 분석, 외래교잡염소와의 유전적 구조 및 평가 같은 유전학적 연구는 현재 부족한 실정이므로 본 연구는 재래흑염소와 외래교잡염소의 유전적 구조 및 혼합 양상 분석을 위해 실시하였다. 11개의 Microsatellite (MS) marker를 활용하여 재래흑염소 261두, 외래교잡염소 94두의 대립유전자형을 분석하였으며, 이를 통해 기초통계량 및 유전적 구조 및 혼합 양상 분석을 수행하였다. 그 결과 연구에 활용된 marker의 유전적 다양성 기초통계량 결과 재래흑염소의 observed heterozygosity, polymorphism information content (PIC) 수치가 각각 0.388 ~ 0.772, 0.362 ~ 0.754이며, 외래교잡염소 집단의 observed heterozygosity, PIC 수치는 각각 0.553 ~ 0.840, 0.664 ~ 0.915로 확인되어, 유전적 다양성 분석을 수행하기에 충분한 수치를 나타내었다. 또한, 재래흑염소 주요 대립유전자형 빈도와 외래교잡염소의 주요 대립유전자형 빈도를 비교하였을 때, marker별 대립유전자형의 빈도 분포에서 뚜렷한 차이를 보여, 두 집단 간, 재래흑염소 계통 간 대립유전자형 의 빈도 분포로 인한 유전적 구조의 차이를 확인하였다. 그리고 STRUCTURE 결과, K = 2가 최적의 K로 계산되었으며, 재래흑염소와 외래교잡염소 간의 유전적 혼합은 존재하지 않는 것으로 판단되었다. 따라서 본 연구 결과는 재래흑염소의 고유성을 제시할 수 있는 과학적 근거자료이며, 재래흑염 소 계통 간의 식별을 위한 기초 자료로 활용 가능할 것이다.

Tricholoma matsutake is a commercially valuable fungus that produces gourmet mushrooms. The artificial cultivation method that is currently used to produce fruiting bodies involves the transplantation of pine trees infected with T. matsutake from their natural habitats and the successful infection of young pine trees and mushroom production have been reported in Hongcheon. In this study, T. matsutake genetic diversity and relationships in this region were investigated. A genotype analysis was conducted on 25 fruiting bodies collected from an area approximately 2.56 km2 in size using 10 simple sequence repeat (SSR) markers. The analysis revealed 25 genotypes and 23 alleles with mean observed (HO) and expected (HE) heterozygosities of 0.312 and 0.293, respectively. The average polymorphism information content (PIC), which indicates marker polymorphism, was 0.257. A phylogenetic analysis showed no clear correlation between collection location and genetic distance; a spatial autocorrelation analysis indicated no significant genetic structure within 1600 m; and the Mantel test showed no significant correlation between genetic and geographic distances. These results were consistent with previous reports on Japanese populations. This study provides insights into the genetic diversity and structure of T. matsutake at a local scale and highlights the need for further studies across broader geographical ranges.

This study focuses on developing diagnostic compositions, kits, and information provision methods for identifying species-specific genes in domestically residing Reticulitermes speratus and Reticulitermes kanmonensis, as well as the recently introduced Cryptotermes domesticus. The core innovation of this invention lies in the utilization of species-specific genetic markers to facilitate rapid and accurate species identification using a PCR (polymerase chain reaction)-based diagnostic technique. This approach enables swift identification of termites at quarantine stages, contributing to efficient management of imported goods and minimizing ecological and economic damages caused by termites. Through genome analysis of termites, this research has identified candidate species-specific genetic markers, developed diagnostic compositions and kits based on these markers, and proposed a rapid diagnostic method capable of determining termite species within a day, optimally within three hours. This invention provides a groundbreaking tool for termite management and research, significantly contributing to pest control and biodiversity conservation efforts.

The pear pest, Cacopsylla jukyungi (Hemiptera: Psyllidae), is one of the most damaging insect to commercial pears in South Korea. In this study, we developed eight microsatellite markers specific to C. jukyungi and genotyped 132 individuals collected from 11 localities throughout South Korea. Populations showed lower observed heterozygosity than expected heterozygosity and slightly or highly positive values of inbreeding coefficients, suggesting that C. jukyungi is subjected to inbreeding. The nationwide expansion of pear orchards and the replacement with a popular new cultivar during the last 50 years, which may have accompanied the spread of C. jukyungi-bearing pear grafts and scions, are likely sources of such facilitated dispersal. Thus, a management strategy against unintended anthropogenic dispersal of the pear psyllid will be required for better control of C. jukyungi.

In Korea, both Sympetrum depressiusculum Sélys, 1841 (Odonata: Libellulidae) and Sympetrum frequens Sélys, 1883 are recorded. However, the identity of Korean populations and the validity of listing the two species have not yet been settled. In this study, we collected 74 individuals from Kroea, Russia, the Netherlands, and Japan. These were sequenced for COI, 16S rRNA, and ITS region. Major morphological characters and phylogenetic, network, and structure analyses all consistently suggest that Korean populations form a single species. Consequently, it could be valid to treat Korean populations as one species, S. depressiusculum, by applying the senior name.

Cucurbita is one of the crops with high demand in the world. Securing breeding sources is crucial and fundamental to any plant breeding program. This study was conducted to investigate the characterization of phenotypic traits and phylogenetic classification of germplasm provided by the National Agrobiodiversity Center of RDA. Fourteen phenotypic traits were measured in 199 accessions of Cucurbita germplasm. Among these germplasm, 92 accessions of C. moschata, 34 accessions of C. maxima, and 73 accessions of C. pepo were classified using the KASP marker from the Seed Industry Center. It was confirmed that there were five classifications in C. moschata, five in C. maxima and four in the C. pepo. The results of this study provide fundamental data on Cucurbita germplasm and are expected to be useful in breeding programs.

A number of Korean Chicken breeds were registered in Domestic Animal Diversity Information System (DAD-IS, http://dad.fao.org/) of the Food and Agriculture Organization (FAO). Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. Therefore, this study aimed to analysis the genetic diversity and relationship of 22 Korean Chicken breeds using 12 microsatellite (MS) markers. The mean number of alleles for each variety was 5.52, ranging from a 3.75 (Leghorn F; NF) to a 7.0 (Ross). The most diverse breed was the Hanhyup3 (HCC), which had the highest expected heterozygosity (HExp) (0.754) and polymorphic information content (PIC) (0.711). The NF was the least diverse population, having the lowest HExp (0.467) and PIC (0.413). As a result of the principal coordinates analysis (PCoA) and factorial correspondence analysis (FCA) confirmed that Hy-line Brown (HL) and Lohmann Brown (LO) are very close to each other and that Leghorn and Rhode Island Red (RIR) are clearly distinguished from other groups. Thus, the reliability and power of identification using 12 types of MS markers were improved, and the genetic diversity and probability of individual discrimination were confirmed through statistical analysis. This study is expected to be used as basic data for the identification of Korean chicken breeds, and our results indicated that these multiplex PCR marker sets will have considerable applications in population genetic structure analysis.

본 연구에서는 유럽 양송이 자원들을 SSR marker를 통 해 유전적 다양성과 집단 구조, 유전적 분화에 대하여 분석하였다. 본 연구에서 유럽의 양송이 자원들은 유전적 거리기반의 4개의 그룹으로 나뉘었고 집단구조 분석을 통하여 2개의 subpopulation으로 이루어져 있었다. 본 연구에서 사용한 SSR 마커로 유럽의 양송이 자원들은 지리적 그리고 갓색으로 구분되지 않았다. 유전적 다양성은 유전적 거리기반의 그룹에서는 Group 4, 집단구조 분석을 통한 subpopulation에서는 Pop. 2의 다양성이 높았다. 그리고 양송이 자원들은 유전적 분화가 매우 낮았다. 본 연구의 결과는 차후 양송이의 육종 등에 이용 할 수 있을 것이다.

The aim of this study was to assess the levels of genetic diversity and relationships of Korean native black goat (n = 58), compared with the exotic breed, Boer (n = 97). For the analysis of genetic characterization 11 microsatellite markers (MAF065, INRA063, CSRD247, OarFCB20, SRCRSP5, INRA006, ILSTS008, ILSTS011, INRA005, ILSTS087, SRCRSP8) were genotyped. The number of alleles was observed 3 (INRA005) to 10 (SRCRSP8) each markers. The mean expected and observed heterozygosity (Hexp and Hobs) and polymorphism information content (PIC) for the Korean native black goat breed varied from 0.551 to 0.860, 0.517 to 0.948 and 0.464 to 0.835, respectively. Principal Components Analysis (PCoA) and FCA results showed that Korean native black goat breed was confirmed to be clearly separated from bore breed. These results were scientific evidence that Korean native black goat represents a unique and valuable animal genetic resource.

In this study, we developed ten microsatellite markers specific to L. angelina using the Illumina NextSeq 500 platform. Forty-three individuals of L. angelina collected from three localities in South Korea were genotyped to validate these markers and to preliminarily assess population genetic characteristics. The observed number of alleles, observed heterozygosity (HO), and expected heterozygosity (HE) at a locus ranged from 4–13, 0.211–0.950, and 0.659–0.871 in the population with the largest sample size (20 individuals), respectively, thereby validating the suitability of the markers for population analyses. Our preliminarily assessment of the population genetic characteristics indicates the presence of inbreeding in all populations, an isolation of the most geographically distant population (Seocheon), and lower HO than HE. The microsatellite markers developed in this study will be useful for studying the population genetics of L. angelina collected from additional sites in South Korea and from other regions. †These authors contributed equally to this paper.

In this study, we developed 11 microsatellite markers specific to A. crataegi using NGS to investigate the genetic relationships of A. crataegi populations from South Korea to circumferential Asian countries (China, Russia, Mongolia, and Japan). Further, two mitochondrial DNA (mtDNA) gene segments (COI and CytB) were sequenced from the samples. The population- and individual-based Principal Coordinates and STRUCTURE analyses collectively suggested that the South Korean population of A. crataegi is most differentiated from the Japanese population, whereas it was closer to Mongolian and Chinese populations. These results collectively suggest that northern populations, in particular, Mongolian populations can be considered as the most genetically compatible one as donee population, when the reintroduction program is launched. †These authors contributed equally to this paper.