Purpose: This study aims to identify the degree of intention to hand hygiene and hand hygiene behavior in new nurses based on education experience on infection control. Method: The participants comprised 108 new nurses working in a general hospital in S city, Korea. Data were collected from September to December, 2020. Data were analyzed using descriptive statistics, independent t-test, analysis of variance (ANOVA), and Pearson correlation with SPSS 26.0 program. Results: There were statistically significant differences in intention to hand hygiene (F=3.39, p=.021) and hand hygiene behavior (F=3.33, p=.022) depending on education experience related to infection control during undergraduate courses. Hand hygiene intention (F=4.41, p=.008) and hand hygiene behavior (F=4.13, p=.008) showed statistically significant differences depending on educational satisfaction with infection control during undergraduate courses. There were significant positive correlations between intention to hand hygiene and hand hygiene behavior (r=.21, p=.026). Conclusion: This suggests that education in infection control can reinforce intention to hand hygiene and hand hygiene behavior in new nurses.